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Tabletlerde Mebendazolün Spektroflorimetrik Tayini

Spectrofluorimetric Assay ofMebendazole in Tablets

Journal Name:

  • Gazi Eğitim Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In this study a spectrofluorimetric method has been developed for assay of mebendazole (MBZ) drug active compound in tablets. For this purpose; taking measured fluorescence intensities of mebendazole drug active compound in various solvents, it was determined that suitable solvent was dimethyl sulfoxide and the wavelengths of excitation and emission were 390 nm and 473,6 nm, respectively. Calibration graphs were drawn and the fluorescence intensity was linearly related to the drug concentration which is between 0-2,5 mg/L (R 2 = 0,9993). Mebendazole in Vermazol tablet was determined both from calibration graph and from standard addition method. The validity of the method was tested by the recovery studies of standard addition to pharmaceuticals and the result was found to be satisfactory. The results obtained from spektrofluorometric method were compared with official USP 24 method and were in good agreement and no significant difference between the two methods at 95 % confidence level was found. The proposed method is simple, accurate and sufficiently precise for quality control purposes in routine analysis of mebendazole in drugs.
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Abstract (Original Language): 
Bu çalışmada, tabletlerde mebendazol (MBZ) ilaç etken maddesinin tayini için spektroflorimetrik bir yöntem geliştirildi. Bu amaçla, mebendazol ilaç etken maddesinin çeşitli çözücülerde floresans spektrumlarıalınarak en uygun çözücünün dimetil sülfoksit ve bu çözücü ortamında uyarma (λuy) ve emisyon (λem) dalga boylarının sırasıyla 390 nm ve 473,6 nm olduğu belirlendi. Kalibrasyon grafikleri çizildi ve bunların 0-2,5 mg/L derişim aralığında doğrusal olduğu görüldü (R 2 = 0,9993). Doğrudan kalibrasyon grafiklerinden ve standart katma yöntemiyle Vermazol tabletindeki mebendazol miktarı tayin edildi. Geliştirilen spektroflorimetrik yöntemle elde edilen sonuçlar, standart USP 24 yöntemi ile elde edilen sonuçlarla karşılaştırıldıve iki yöntem arasında % 95 güven seviyesinde anlamlıbir fark olmadığıtespit edildi. Önerilen yöntemin, ilaçlarda rutin mebendazol analizinde kalite kontrol amacıyla kullanılabilecek kolay, doğru ve kesinliği yüksek bir yöntem olduğu belirlendi.
FULL TEXT (PDF): 
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  • 3
159-174
  • Turkish

REFERENCES

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