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Ratlarda sol kolon greftinde eritropoetinin iskemi üzerine koruyucu etkileri

The protective effects of erythropoietin on ischemia in the left colonic graft in rats

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

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Abstract (2. Language): 
Segments from the stomach, jejunum, ileum and colon may be used for esophageal replacement when irreversible injury of the esophagus prevents passage. Of these techniques colon interposition is the most preferred method. Reperfusion damage develops at a certain rate in colon grafts used for esophageal replacement as with all other surgical methods where grafts are used. It is known that erythropoietin hormone may have a tissue-protective effect in ischemia/ reperfusion. Ischemic changes developing in mucosal cells in the graft ends after creating a left colon graft in rats and the effect of intraperitoneal erythropoietin administration on these changes were investigated in the present study. A total of 56 subjects were randomly divided into four main groups of Sham (n=14), Sham+erythropoietin (n=14), colon graft (n=14) and colon graft+erythropoietin (n=14), and each group was divided into 2 subgroups. Histopathological examination was performed to the first subgroup one day and to the second subgroup 7 days after reoperation. There was significantly much more mucosal damage in the colon graft group in comparison to the postoperative day 1 and 7 proximal ends between the colon graft and colon graft+erythropoietin groups. Results of the present study showed that intraperitoneal erythropoietin administration significantly decreased mucosal damage in rats in which a colon graft was prepared.
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Abstract (Original Language): 
Ösefagusun geriye dönüşsüz bir şekilde hasar görerek pasajı engellediği durumlarda, ösefagus replasmanı amacıyla mide, jejunum, ileum ve kolon segmentleri kullanılabilmektedir. Bu tekniklerden kolon interpozisyonu en sık tercih edilen yöntemdir. Greftlerin kullanıldığı tüm diğer cerrahi yöntemler gibi ösefagus replasmanı amaçlı kullanılan kolon greftlerinde de reperfüzyon hasarı belirli oranda gelişmektedir. Eritropoetin hormonunun iskemi/reperfüzyonda dokuyu koruyucu bir etki gösterebileceği bilinmektedir. Bu çalışmada ratlarda, sol kolon grefti oluşturulduktan sonra greftin uç kısımlarında meydana gelen mukozal hücrelerdeki iskemik değişiklikler ile intraperitoneal eritropoetin uygulamasının bu değişiklikler üzerine olan etkileri araştırılmıştır. Toplam 56 denek rastgele Sham (n=14), Sham+eritropoetin (n=14), kolon grefti (n=14), kolon grefti+eritropoetin (n=14) olmak üzere dört ana gruba, her grup da kendi içinde 2 alt gruba ayrıldı. Birinci alt gruba 1 gün sonra, 2. alt gruba ise 7 gün sonra reoperasyon sonrası histopatolojik inceleme yapıldı. Kolon grefti ve kolon grefti+eritropoetin grupları arasında postoperatif 1. gün ve 7. gün proksimal uçlarının değerlendirilmesinde mukozal hasarın kolon grefti grubunda anlamlı olarak daha yüksek olduğu görüldü. Bu çalışma, preoperatif intraperitoneal eritropoetin uygulamasının, kolon grefti hazırlanmış ratlarda mukozal hasarlanmayı belirgin olarak azalttığını göstermektedir.
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  • 4
233-237
  • Turkish

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References: 

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