You are here

Home » Content

Klorprifos-etü'in. HEPG2 hücre dizüerinde hücre canlılığına etkisi ve melatoninin koruyucu etkisi

The effect of chlorpyrifos-ethyl on cell viability in HEPG2 cell lines and protective role of melatonin

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Chlorpyrifos-ethyl (CE) is one of the organophosphate insecticies which are commonly used . İn this study, we investigated the cytotoxic effect of Chlorpyrifos-ethyl onto HepG2 liver cells and melatonin's protecting effect. The working groups were arranged as shown : 1. Control group (Other group's viability percentage were evaluated comparing to this group) 2. CE group (The treatment was made with different dosages of CE) 3. M+CE group (Melatonin was added to the different dosages of CE) HepG2 cells were seeded in 96-well plates (3x105 cells/well). Each of 3x105 cells were put into 96 well Petri cups. After 24 hours the cells in CE group were incubated with Chlorpyrifos-ethyl with the doses of 1080, 540, 270, 135, 67.5, 33.7, 16.8, 8.4, 4.2, 2.1, 1 and 0.5 microg/ml in 37°C for 72 hours. On the other hand, the HepG2 cells in the M+CE group were pre-incubated with melatonin with the dose of 10 mg/l for an hours and were handled with the chancing CE concentration for 72 hours. After that, the cell viability of CE and M+CE groups were established. The viability of HepG2 cells in CE treated group was found lower than the cels which were chosen to control.This was found related to the CE concentration. As the Chlorpyrifosethl concentration decreases the cytotoxic rate decreases (Kruskal-Wallis Test p=0.024). The viability of the HepG2 cells incubated with mela-tonin for one hour before the CE application found to increased (Mann Whitney U Test, p<0,05). As a result; it was concluded that when CE was superimposed onto HepG2 liver cell series in in vitro condi¬tions, it showed significantly cytotoxical effect, and melatonin protected the liver cells from the cytotoxic effect of CE.
Bookmark/Search this post with
Abstract (Original Language): 
Klorprifos-etil (KE), yaygın olarak kullanılan organofosfat insektisitlerden biridir. Bu çalışmada, KE'in insan karaciğer hücre dizisi HepG2 üzerine sitotoksik etkisini ve melatoninin koruyucu etkisini araştırdık. Çalışma grupları şu şekilde organize edildi: 1. Kontro l grubu (Diğer grupların canlılık oranları bu gruba göre değerlendirildi.) 2. KE grubu (Değişik dozlarda KE ile muamele edildi.) 3. Melatonin+ KE grubu (Değişik dozlarda KE'ye ek olarak melatonin eklendi.) Hücreler 96 well petri kaplarına her well'de 3x105 olacak şekilde ekildi. 24 saat sonra KE grubundaki hücreler 1080, 540, 270, 135, 67.5, 33.7, 16.8, 8.4, 4.2, 2.1, 1 ve 0.5 mikrogram/mlO konsantrasyonlarında KE ile 72 saat 37 oC'de inkübe edildiler. Melatonin+KE grubundaki HepG2 hücreleri ise1 saat 10 mg/l konsantrasyonunda melatonin ile ön inkübasyona alındıktan sonra değişen KE konsantrasyonları ile 72 saat muamele edildiler. Daha sonra KE ve KE+M gruplarında hücre canlılığı belirlendi. KE verilen HepG2 hücrelerinin canlılığı kontrol grubundaki hücrelere oranla anlamlı olarak düşük bulunmuştur. KE'nin sitotoksisitesi uygulanan KE konsantrasyonuyla ilişkili bulunmuştur. KE konsantrasyonu düştükçe sitotoksisite de buna paralel olarak azalmıştır (Kruskal-Wallis Test p=0.024). Buna ek olarak çalış¬mamızda melatoninle bir saat inkübe edilen HepG2 hücrelerine KE uygulaması melatonin uygulanmayan gruba oranla hücre canlılığını anlamlı olarak artmıştır (Mann Whitney U Test, p<0.05). Sonuç olarak KE'in insan karaciğer hücre dizisi HepG2 üzerine in vitro şartlarda uygulanmasıyla, bu hücrel¬er üzerinde anlamlı olarak sitotoksik etki gösterdiği, melatonin uygulanması ise karaciğer hücre dizilerini klorprifos-etil sitoksisitesinden önemli derecede koruduğu sonucuna varılmıştır.
FULL TEXT (PDF): 
PDF icon arastirmax-klorprifos-etuin.hepg2-hucre-dizuerinde-hucre-canliligina-etkisi-melatoninin-koruyucu-etkisi.pdf
  • 3
39-43
  • Turkish

REFERENCES

References: 

1. Hunter DL. , Lassiter TL., Padilla S. Gestational exposure to chlorpyrifos : comparative distribution of trichloropyridinol in the fetus and dam. Toxicol Appl Pharmacol. 1999;158(1) : 16-23.
2. Muscarella DE, Keown JF, Bloom SE. Evaluation of the genotoxic and embriyotoxic potential of chlorpyrifos and its metabolites in vivo and in vitro.Environ Mutagen. 1984;
6(1) : 13-23.
3. Li WF,Furlong CE,Costa LG. Paraoxonase protects aganist clorprifos toxicity in mice.Toxicol Lett 1995;76(3):
219-26.
4. Bigbee JW, Sharma KV, Gupta JJ, Dupree JL. Morfogenik role for acetylcholinesterase in axonal out¬growth during neural development. Environ Health Perspect. 1999; 1:81-8.
5. Dwivedi PD, Mukul D, Khanna SK. Role of cytochrome p-450 in quinalphos toxicity : Effect on hepatic and brain antioksidant enzymes in rats. Food and Chemical
Toxicology. 1998; 36 , 437-44.
6. Stephen B, Kyle L, Yong X, Cynthia A, Donald E, Earl F, James E. Role of oxidative stress in the mechanism of dieldrin's hepatotoxicity. Annals of Clinical and
Laboratory Science. 1997; 27(3) : 196-208.
7. KazançMB.Antioksidan vitaminler. Sendrom. Temmuz, 1997;14-23
8. Richardson RJ. Assessment of the neurotoxic potential of chlorpyrifos relative to other organophosphorous com¬pounds : a critical review of the literature.J Toxicol Environ Health. 1995; 44(2): 135-65.
9. Bagchi D, Bagchi M, Hassoun EA, Stohs SJ. In vitro and in vivo generation of reactive oxygen species, DNA dam¬age and lactate dehydrogenase leakage by selected pesti¬cides. Toxicology. 1995; 104(1-3) : 129-40.
10. Cheeseman KH, Slater TF. An introduction to free rad¬ical biochemistry . Br. Med. Bull. 1993; 49(3) 479-80.
11.
Özgüne
r F, Delibaş N, Özcankaya R, Karaca H, Koyu A. Yaşla azalan melatoninin oksidatif hasar ve mental yetil¬erle ilişkisi. Yeni Tıp Dergisi. 1996; 13(6) : 367-69.
12. Pieri C, Marra M, Moroni F, Recchioni R, Marcheselli F. Melatonin: peroxyl radical scavenger more effective than vitamin E. Life Sci. 1994; 55:PL271-6.
13. Datta C, Gupta J, Sarkar A, Sengupta D. Effects of organophosphorus insecticide phosphomidon on antioxi-dant defence components of human erythrocyte and plas¬ma. Indian Journal of Experimental Biology. 1992; 30 : 65¬67.
14. Prasanta KM, Anand K. Dimethoate inhibits extrathy-roidal 5' - monodeiodination of thyroxine to 3,3', 5- tri-iodothyronine in mice : the possible involvement of the lipid peroxidative process. Toxicology Letters 1997; 91: 1¬6.
15. Thrasher JD, Madison R, Broughton A. Immunologic abnormalities in humans exposed to chlorpyrifos : prelimi¬nary observations. Arch Environ Health . 1993; 48(2) : 89¬93.
16. Pond AL, Chambers HW, Chambers JE. Organophosphate detoxication potential of various rat tis¬sues via A - esterase and aliesterase activities. Toxicol
Lett. 1995; 78(3) : 245-52.
17. Sultatos LG. Metabolic activation of the organophos-phorus insecticides chlorpyrifos and fenitrotion by per¬fused rat liver. Toxicology. 1991; 68(1) : 1-9.
18. Li W, Casida JE. Orgarophosphorus neuropathy target esterase inhibitors selectively block at growth of nourite-like and cell proceeees in cultured cells. Toxicol Lett. 1995; 76(3):219-2.
19.
Özgüne
r F, Özcankaya R, Delibaş N, Koyu A, Çalışkan S. Melatonin ve klinik önemi . SDÜ Tıp Fakültesi Dergisi. 1995; 2(4) : 1-6.
20. Tan D, Reiter RJ, Chen LD, Poeggeler B, Manchester LC, Barlow-Walden LR. Both physiological and pharma¬cological levels of melatonin reduce DNA adduct forma¬tion induced by the chemical carcinogen safrole. Carcinogenesis . 1994; 15:215-8.
21.
Gro
s V, Arndt H, Andus TPalitzsch KO, Schölnerick J. Free radicals in inflammatory bowel diseases .Pathophysiology and therapeutic implications. Hepato-Gastroenterology. 41:320-7.
22.
Önc
ü M, F. Gültekin, E. Karaöz, İ. Altuntaş, N. Delibaş. Klorprifos-etil tarafından oluşturulan oksitatif hasarın sıçan karaciğerine etkileri. T. Klin. Tıp Bilimleri.
2002; 22(1): 50-5.
23. Gultekin F, Delibas N, Arican A, Sutcu R, Kilinc I,
Kaptanagasi M. Investigation of the relation between LD50 and lipoperoxidative effect of certain agrochemical.
Biomedical Research 2000; 11: 67-71.
24. Gultekin F, Ozturk M, Akdogan M. The effect of
organophosphate insecticide chlorpyrifos-ethyl on lipid peroxidation and antioxidant enzymes (in vitro). Archies of
Toxicology 2000; 74: 533-538.
25. Gultekin F, Delibas N, Yasar S, Kilinc I. In vivo changes in antioxidant systems and protective role of mela-tonin and a combination of vitamin C and vitamin E on
Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2003:10-( 3 ) / 3 9 - 4 3
Patat, Klorprifos-etil'in hücre canlılığına etkisi
43
oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Archives of Toxicology 2001; 75: 88-96.
26. Gultekin F, Kaleli S, Altuntas I, Oncu M, Gokcimen A, Sutcu R. The in vivo lipoperoxidative effect of chlorpyri-fos-ethyl on testis tissue of rats. Genel Tıp Dergisi 2000; 10: 147-152.
27. Gultekin F, Delibas N, Kutluhan S, Akdogan M, Kilinc
I, Sutcu R. The changes in antioxidant system caused by chlorpyrifos-ethyl in rat brain and protective effect of mela-tonin and vitamin C + vitamin E. Selçuk Üniversitesi Tıp
Fakültesi Dergisi 2001; 17: 79-86.
28. Oncu M, Gultekin F, Karaoz E, Altuntas I, Delibas N. Nephrotoxicity in rats induced by chlorpryfos-ethyl and ameliorating effects of antioxidants. Human and Experimental Toxicology, 21: 223-230, 2002.

Thank you for copying data from http://www.arastirmax.com