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SAĞLIKLI İNSANLARDA ERİTROSİT MEMBRANI Na+-K+ ATPaz VE Ca2+ ATPaz AKTİVİTELERİNE VE SİALİK ASİD DÜZEYLERİNE YAŞLANMANIN ETKİSİ

This study was planned to investigate the changse on erythrocyte membrane Na+-K+ATPaz ye Ca2+ ATPaz activities and sialic acid levels that may be related to aging

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 1999

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
membrane Na+-K+ATPaz ye Ca2+ ATPaz activities and sialic acid levels that may be related to aging. An insignifİcant decrease was obtained in the erythrocyte membrane Na+-K> ATPase and Ca2+ ATPase activities of 14 old subjects with a mean age of 64 according to the enzyme activities of the 14 yoııng subjects whose mean age was 28. The intracellular and extracellularNa+ and K+ concentrati-ons and îhe plasma and erythrocyte membrane sialic levels were found with a tendency to inc-rease in the elder subjects but only the plasma Na+ increase was significant. According to these fındings, it İs thougt these two enzyme activities were not affected with increasing age and the aging process do not make some negative changes on celi volume and homeostasis mechanisms and on membrane structure.
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Abstract (Original Language): 
Bu çalışma, eritrosit membram Na*--K+ ATPaz ve Ca2+ ATPaz aktivİteleri ile sialik asid düzeyleri üzerine yaşa bağımlı olabilecek değişiklikleri araştırmak için planlanmışın". Yaş ortalaması 64 olan 14 yaşlı olgunun eritrosit membram Na+-K+ ATPaz ve Ca3+ ATPaz aktİvitelerinde, yaş ortalaması 28 olan 14 gencin enzim aktivitelerine göre anlamlı olmayan azalma saptandı. Hücre içi ve dışı Na+ ve K+ konsantrasyonları ile plazma ve eritrosit membram sialik asidm düzeyleri yaşlılarda artmaya eğilimli bulundu, ancak yalnız plazma Na+ artışı anlamlı imdi. Bu bulgulara göre; her iki enzim aktivitelerinin ilerleyen yaş ile etkilenmediği ve yaşlanma olayının hücre hacmi ve homeostazisi mekanizmalarında ve membran yapısında organizma aleyhine olabilecek değişikliklere yol açmadığı düşünüldü.
FULL TEXT (PDF): 
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  • 2
129-133
  • Turkish

REFERENCES

References: 

1. Allalouf D„ Komios L., Notman J., Halbrecht I., Le-vinsky H.: Sialic acid content and sialyltransferase acti-vity in human lymphocytes with advancing age. Mech Ageing Dev 44:45 (1988).
2. Amano M., Imata K., Suzuki K., Nakaoka H., Fuji J.: Agelated reduction İn the number of rabbit erythrocyte Na, K-ATPase. Tohoku J Exy Med 159:131 (1989)
3. Bozzo C, Goria M., Marena S., Velgia F., Pagano G.: Lyraphocyte Na-K-ATPase is reduced in aged peopie. Metab. 8:808 (1990).
4. Cumbcrbatch M., Morgan D.B.: Relations between sodi-um transport and sodium concentration in human ery-thaqrocytes in health and disease. Clin Sci. 60:555 (1981).
5. Davis P.J., Blas S.D: In vitro stimulation of human red biood celi Ca2+ ATPase by thyroid hormone. Biochem Biophys Res Commun 99:1073 (1981).
6. Denyy P.C., Denny P.A., Aİlerton S.E.: Determination of sialic acid using 2-thiobarbituric acid in the absence of hazardous sodiium arsenİte. Ciin Chim Açta 131:333 (1983).
7. Est M., Erjavec E., Krzan M.: Age-related changes chan-ges of erythrocyte Na+"K+ATPase activity. lugoslav Physiol Phaımacol Açta 25:35 , (1989).
8. Hennesy J. F.: Vascular tissue Na+"K+ ATPase activity and aging in the F344 rat. Mech Ageing Dev 43:153, (1988).
9. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J.: Protein measurement with tbe Folinphenol reagent. J Bi-ol Chem 193:265 (1951).
10. Naylor G.J., Dick D.A.T., VVorail E.P., Dick E.G., Dick P., Boardman L.: Changes in theerytrocyte sodium pump with age. Gerontology 23:256 (1977).
11. Paci A., Cocci F. Cristofanİ R., Balzan S., Mezzasalma L., Piras F., Giachetti M., Ghione S.: Comparison of int-ra erythrocyte and intraleucocyte sodium content and erythrocyte fragility in normotensive subjects. J Nuclear Med Ailied Sci 32:101 (1988).
12. Rahman M., Koh H„ Primera M.I., Del Grmeco F., Qu-intanilia A.P.: Na-K-Adenosine triphosphatase and cati-on content in the erythrocyte in essentiaî hypertension. J Lab Ciin Med 107:337 (1986).
13. Samaja M., Rubinacci A., Molterlini R., De Ponti A., Postinaro N.: Red celi ageing and active caicium transport. Exp Gerontol 25:279, (1990).
14. Schmalzing G„ Pfaff E., Breyer-Pfaff U.: Red celi ouba-in binding sites, Na+_K+ ATPase and intracellular Na+ as individual charactcristics. Life Sci 29:371 (1981).
15. Schulte B.A., Schmiedt R.A.: Lateral Wall Na+-K+ATPase and endocochlear potentials decline with agen in quietreared gerbils. Hearing Res 61:35 (1992).
16. Simat B.M., Morley J.E., From A.H.L., et al.: Variables affecting measurement of human red celi Na+_K+ATPase activity teehnieal factors feeding, aging. Am J Clin Nutr 40:339, (1984).
17. Stefenelli N., Klotz H., Engel A., Bauer P.: Serum sialic asid in malignant tumors, bacterial infeetions and ehro-nic liver diseases. S Cancer Res Clin Oncol 109:55 (1985).
18. Stibler H., Sydow O.: The sialic acid and galactose concentrations in erythrocyte membranes in patients with mytonic dystrpphy, limp-girdlc and facioscapulo humeral dystrophy. J Neuroİogical Sci 59:389 (1983).
19. Sydow G.: A simplified quick metod for determination of sialic acid in serum. Biomed Biochim Açta 44:1721 (1985).
20. Taussky H.H., Shorr E.: A miero colorimetric method for determination of inorganic phosphorus. J Biol Chem 202:675 (1953).
21. Tozzi-Ciancareİli M.G., Fedele F., Tozzi E., et al : Age-dependent changes in human erythrocyte properties. Ciin Hemorheology 9:999 (1989).
22. Wood L., Beutier E.: Temperature dependence of sodium potassium activated erythrocyte adenosine triphosphatase. J Lab Clin Med 70:288, (1967).

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