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Hemodiyaliz, Periton Diyalizi ve Prediyaliz Hastalarda Gizli Hepatit B Enfeksiyonunun Polimeraz Zincir Reaksiyonu Yöntemi ile Araştırılması

The Investigation of Occult Hepatitis B Infection in Hemodialysis, Peritoneal Dialysis, and Predialysis Patients by the Polymerase Chain Reaction Method

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The presence of hepatitis B virus DNA in case of negative hepatitis B surface antigen in serum is known as occult hepatitis B. Although the exact prevalance is not known, there are many reports indicating that occult hepatitis B is more frequently encountered in patients undergoing hemodialysis treatment. The aim of this study was to investigate the prevalence of occult hepatitis B in patients on hemodialysis or peritoneal dialysis, or with predialytic chronic renal failure. A total of 174 HBsAg-negative sera were included in the study. HBV-DNA was detected by polymerase chain reaction (PCR) in 2.6% of hemodialysis patients and 1.8% of peritoneal dialysis patients. In contrast, there was no occult hepatitis B virus infection in non-dialyzed patients with chronic renal failure. According to the liver enzymes and serum C-reactive protein levels, there was no statistically difference between the occult hepatitis B positive patients and the negative patients. In conclusion, although the rate was low, occult hepatitis B infections was detected among dialysis patients in our study. Therefore, despite serological analysis, both hemodialysis and peritoneal dialysis patients should be screened for occult HBV infections with a more sensitive test such as PCR, in order to prevent the dialysis-mediated transmision risk of HBV infection.
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Abstract (Original Language): 
Serumda hepatit B yüzey antijeninin (HBsAg) negatif olduğu durumlarda, hepatit B virüsü DNA (HBV-DNA)’sının varlığı gizli hepatit B olarak bilinmektedir. Gizli hepatit B’nin prevalansı tam olarak bilinmemesine rağmen, hemodiyaliz tedavisi uygulanan hastalarda daha sık olduğunu bildiren çok sayıda araştırma vardır. Bu çalışmada, hemodiyaliz ve periton diyalizi hastaları ile kronik böbrek yetersizliği olup henüz diyaliz tedavisi başlanmamış hastalarda (prediyaliz) gizli hepatit B prevalansı araştırıldı. Çalışmaya HBsAg-negatif olan toplam 174 hasta alındı. Polimeraz zincir reaksiyonu (PZR) yöntemi ile hemodiyaliz hastalarının %2.6’sında, periton diyalizi hastalarının %1.8’inde HBV-DNA varlığı saptandı. Buna karşılık, prediyalitik hastalarda gizli hepatit B enfeksiyonu saptanmadı. Gizli HBV enfeksiyonu saptanan hastalarla diğer hastalar arasında karaciğer enzimlerinin yüksekliği ve serum C-reaktif protein seviyesi yönünden anlamlı düzeyde farklılık saptanmadı. Sonuç olarak, çalışmamızda hemodiyaliz uygulanan hastalarda gizli HBV enfeksiyonunun düşük sıklıkla olsa da görülebileceği saptanmıştır. Hem hemodiyaliz hem de periton diyalizi uygulanan hastalara cihaz kaynaklı HBV bulaşımını önlemek için sadece serolojik testlerle virüs varlığının araştırılmasının yeterli olamayacağı, PZR gibi duyarlılığı daha yüksek testlerle bu hastaların taranmasının uygun olabileceği sonucuna varılmıştır.
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  • 2
55-61
  • Turkish

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DOĞUKAN: Hemodiyaliz, Periton Diyalizi ve Prediyaliz Hastalarda
Gizli Hepatit B Enfeksiyonunun Polimeraz Zincir Reaksiyonu
Yöntemi ile Araştırılması
Cilt/Vol. 18, No, 2, 2009 Sayfa/Page 55-61
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