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PHENOTYPIC DETECTION OF INDUCIBLE CLINDAMYCIN RESISTANCE IN STAPHYLOCOCCUS AUREUS

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Abstract (2. Language): 
Aim skin and soft tissue infections. The resistance to antimicrobial agent among staphylococci is an increasing problem. Clindamycin is considered to be one of the alternative agents in clindamycin resistance among demonstrates a simple and reliable method of 300 specimens like pus, blood, sputum, throat swab, ear swab, high vaginal swab, CSF, urine, pleural fluid, semen, bile, corneal swab, etc. To detect inducible clindamycin resistance, 15 μg erythromycin and 2 μg clindamycin Hinton agar plate at a distance of 15 mm apart (measuring edge to edge). If there is inducible clindamycin resistance, the erythromycin will diffuse through the agar and resistance to clindamycin will be induced, resulting flatt inhibition adjacent to erythromycin disc and giving D of 300 Staphylococcus aureus isolates, 109 strains (36.33%) were found to be MRSA and 191 strains (63.67%) were Non MRSA. Of these, 90 strains (30%) be resistance to erythromycin. These isolates were subjected to D tests shows 11 isolates resistance to both erythromycin and clindamycin indicating constitutive MLS phenotype and isolates, 47 isolates showed positive D- Test indicating. out of these 79 isolates showed positive D- TEST indicating inducible MLSB phenotype while 32 gave negative D test indicating MS phenothpe.
FULL TEXT (PDF): 
267-272

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