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Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme

The Protective Effect of Green Tea Extract on Formaldehyde Toxicity: Genotoxic Evaluation in Swiss Albino Mice

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2015

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Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In the present study, protective effect of green tea extract against genotoxic effects of formaldehyde on Swiss albino mice were investigated. For this aim, we used the frequency of micronucleus, chromosomal aberrations, abnormal metaphase number and mitotic index as indicators of toxicity. Animals were randomly divided into six groups of six animals each. Green tea extract was applied with two different doses (50 and 150 mg/kg) and formaldehyde was treated with the dosage of 200 mg/kg. All mice were sacrificed at the end of treatment period and the related tissues were isolated. Formaldehyde alone-treated mice presented higher frequencies micronucleus, chrosomal aberration and abnormal metaphases compared with the controls; moreover, the mitotic index was lower than in controls. Oral treatment with gren tea extract significantly ameliorated the indices of genotoxicity induced by formaldehyde. Both doses of Green Tea extract tested provided significant protection against formaldehyde-induced toxicity, and its strongest effect was observed at the dose level of 150 mg/kg of body weight. The results obtained from this study suggest that Green Tea is a effective protective agent against Formaldehyde induced toxicity.
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Abstract (Original Language): 
Bu çalışmada Swiss albino farelerde yeşil çay özütünün formaldehitin genotoksik etkisine karşı koruyucu rolü araştırılmıştır. Bu amaçla mikronukleus sıklığı, kromozomal hasar, anormal metafaz sayısı ve mitotik indeks testleri uygulanmıştır. Fareler her bir grupta altı fare olmak üzere altı gruba ayrılmıştır. Yeşil çay özütü iki doz (50 ve 150 mg/kg) formaldehit ise tek doz (200 mg/kg) halinde uygulanmıştır. Uygulama süresi sonundat tüm farelerden doku izolasyonu gerçekleştirilmiştir. Formaldehit uygulaması yapılan grupta kontrol grubuna kıyasla yüksek oranda mikronukleus, kromozomal hasar ve anormal metafaz gözlenirken; mitotik indeks oranının kontrol grubundan daha düşük olduğu belirlenmiştir. Oral yolla yeşil çay özütü uygulamasının formaldehitin oluşturduğu genotoksisiteyi azalttığı belirlenmiştir. Yeşil çay özütü uygulamasının her iki dozunun da formaldehit tarafından oluşturulan toksisiteye karşı koruyucu etki gösterdiği ve bu etkinin 150 mg/kg dozunda daha etkin olduğu gözlenmiştir. Bu sonuçlar ile yeşil çay özütünün formaldehit tarafından oluşturulan toksisiteye karşı koruyucu bir ajan olduğu belirlenmiştir.
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