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POLIETILEN GLIKOL (PEG 6000) İLAVESININ BAZI KANOLA ÇEŞITLERİNİN VE KANOLA KÜSPESİNİN İN VITRO GAZ ÜRETIMI ÜZERINE ETKILERI

EFFECTS OF POLYETHYLEN GLYCOL (PEG 6000) SUPPLEMENT ON IN VITRO GAS PRODUCTION OF CANOLA HYBRIDS AND CANOLA MEALS

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Abstract (2. Language): 
The aim of this study was to determine the effects of polyethylen glycol on in vitro gas production and in vitro gas production kinetics of some canola hybrids and canola meals. In this study, four canola hybrids (Bristol, Eurol, Capitol and Licrown) and canola meals purchased from market were used. Two rams (SakızxKarayaka) aged 4 with ruminal cannulas were used in gas production technique. All of the feedstuffs were incubated for 3, 6, 9, 12, 24, 48, 72 and 96 hours. All canola varieties had lover gas production levels except for canola meals. The effects of PEG supplement on in vitro gas production, a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and c (gas production rate) of all canola hybrids and canola meals for 3, 6, 9, 12 ,24 and 48 hour incubations were not significant (P>0.05). However, PEG supplementation decreased total gas production (a+b) and in vitro gas production for 72 and 96 hour incubations in Bristol hybrid (P<0.01). Besides, PEG supplemention increased in vitro gas production for 72 hour incubation in Capitol hybrid (P<0.01). In conclusion, PEG supplementation affected in vitro gas productions for 72 and 96 hours incubations and total gas production (a+b) for all the feeds used in this study (P<0.01). PEG addition could not show its effect due to the lower TMP contents in canola hybrids and canola meals used in this study. In vivo experiments should be carried out with feeds rich in tannin by using most appropriate PEG 6000 level.
Abstract (Original Language): 
Bu çalışmanın amacı bazı kanola hibritlerinde ve kanola küspesinde in vitro gaz üretimi ve gaz üretim parametreleri üzerine polietilen glikol ilavesinin etkisini belirlemektir. Çalışmada dört farklı kanola hibriti (Bristol, Eurol, Capitol ve Licrown) ve piyasadan temin edilen kanola küspesi kullanılmıştır. İn vitro gaz üretim tekniğinde dört yaşında rumen kanülü takılmış, 2 baş Sakız-Karayaka melezi koç kullanılmış ve yemler 3, 6, 9, 12, 24, 48, 72 ve 96 saat süre ile inkübasyona bırakılmışlardır. Bütün kanola hibritleri, kanola küspesinden daha düşük gaz üretimine sahip olmuşlardır . Denemede kullanılan yemler arasında 3, 6, 9, 12, 24 ve 48 saatlik inkübasyon sonrasında in vitro gaz üretim miktarları, hemen çözünebilir fraksiyondan oluşan gaz miktarı (a), zamana bağlı oluşan gaz miktarı (b) ve gaz üretim hızı (c) üzerine PEG ilavesinin etkisi önemsiz bulunmuştur (P>0.05). Bununla beraber PEG ilavesinin Bristol için 72 ve 96 saatlik inkübasyonlar sonrasında oluşan in vitro gaz üretimini ve toplam gaz üretimini (a+b) düşürdüğü (P<0.01); Capitol için 72 saatlik inkübasyon sonrasında in vitro gaz üretimini artırdığı saptanmıştır (P<0.01). Sonuç olarak 72 ve 96 saatlik inkübasyon süreleri ile toplam gaz üretimi (a+b) bakımından PEG (6000) ilavesinin denemede kullanılan bütün yemler üzerine etkisi görülmüştür (P<0.01). Çalışmamızda kullanılan kanola hibritleri ve kanola küspesindeki toplam fenolik maddeleri (TFM) içeriklerinin düşük düzeylerde olması nedeniyle PEG ilavesinin etkisi saptanmamıştır. PEG etkisini görebilmek için, TFM içeriğince zengin yemlere en uygun PEG 6000 düzeyinin kullanıldığı in vivo denemelerin yürütülmesi gerekmektedir.
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