You are here

Home » Content

EXPERIMENTAL KEMİK DEFEKTLERİNİN İYİLEŞMESİNDE REKOMBİNANT Bİ YO SENTETİK GROWTH HORMON TEDAVİSİNİN VE OKTREOTİD ASETATIN ROLÜ

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
thetic growth hormone is a synthetic hormone produced with recombinant DMA technique and having the same aminoacid arrangement with hypophysis growth hormone, secreted naturally. Octreotide Acetate is a long-acting somatostatin analogue, in addition to the inhibitor effect of growth hormone release, it is also effective on the whole endocrine, paracrine and otocrine glands. [ n this study, we aimed to investigate the effect of biosynthetic growth hormone on fracture fusion and defect healing. In our study, we have opened 3 mm-diamctcred cavity in right tibia of 36 matured rabbits under general anaesthesia and sutured skin, underskin and pcriost. Then, th4 rabbits were divided into three equal groups. From the beginning of operation for 20 days. 0.08 biosynthetic growth hormone was injected for the first group. 5 microgram/kg octreotide; acetate was injected for the second group, and serum physiologic in the same volumes was injected for the third group. On the 20L^ day, rabbits nere sacrificd, and defective regions were extracted. Kruskal- Wallis statistic test was applied to the data obtained in the result of histopathologic survcry ol specimens. In conclusion, when sponginoz bone formation, bone marrow and the formation of cortex and bone welding were taken up totally, it was found out that there was statistical significance among three groups. While the fastest recovering is seen in the group administered growth hormone, the slowest recovering is seen in the group administered octreotide.
Bookmark/Search this post with
Abstract (Original Language): 
İJiyûScîîttîik bii\UîTjC hüiTTiüIii] Lubü GuLflik salgılanan htpofiz büyüme hormonu ile aynı aminoasit dizilişine sahip, rekombinant DNA teknolojisi ile üretilen sentetik bir hormondur. Oktrcotid asetat ist uzun etkili, somatostatin analogu olup, büyüme hormonu sal mimini inhibe etmesinin vamsıra, tüm endokrin, parakrin ve otokrin bezlerde de clkil idir. Bu çalışmada biyosentetik büyüme hormonunun kırık kaynamasına vc del eki iyileşmesine olan etkisini İncelemeyi amaçiadık. Çalışmamızda 36 adetınatür tavşanın sağ LibiaEanna genel urı es tezi altında 3 mm çapında kaviteler açılarak, cilt, cilt altı ve periost sütürc edildi. Daha sonra tavşanlar 3 eşit gruba ayrılarak operasyon gününden itibaren 2Q gün süreyle; 1. gruba : 0.08 mg/ kg biyosentetik büyüme hormonu. 2- gruba : 5 rntkrogram/ kg oktreotid asetat 3 gruba • Aynı hacimlerde sertim fizyolojik enjeksiyonları yapıldı Tavşanlar 20. günde sak ri fiye edilerek defekt bölgeleri çıkarıldı. Spcsmenlerin histopatolojik olarak incelenmesi neticesinde elde edilen verilere Kruskal- Wallis istatistik testi uygulandı. Sonuçta: kemik kaynaması, üpongioz kemik formasyonu, kemik iliği vc korteksin oluşumu total olarak ele alındığında 3 grup arasında istalisLiksel anlamlılık görülmüştür. Büyüme hormonu verilen grupta en hızlı iyileşme görülürken en yavaş iyileşmenin oktrcotid verilen grupta olduğu
FULL TEXT (PDF): 
PDF icon arastirmax-experimental-kemik-defektlerinin-iyilesmesinde-rekombinant-bi-yo-sentetik-growth-hormon-tedavisinin-oktreotid-asetatin-rolu.pdf
  • 2
1-11
  • Turkish

REFERENCES

References: 

1. Kayaalp O. Tıbbi Farmakoloji Gözden Geçirme Kitabı.Ankara. Hacettepe-Taş Kitapçılık, 1996: 322-323,
2.
Alpasla
n R.Tazc Kırık Onarımı Üzerine Darbcli Elcktromagnetik Alanın Etkileri. Atatürk Üniversitesi Tıp Fakültesi Ortopedi ve Travmatoloji Anabil i m Dalı Doçentlik reyi.Erzurum 1983.
3.
Guytot
ı AC.Textbook of Medical Physiology 5 tli-Ed,Terctime eden. Kazancıgil. Devrim. Ankara, Güven Kıtabcvi Yayınları. l.Baskı, 1978: 308-320.
4.
Koloğl
u S. Endokrinoloji Temel ve Klinik 1. Baskı.Ankara. Medical Network, 1996: 567-975
5.
Sımar
d M, Manthos Hr GiAlDA., Goodyer D. Ontogeny of growth hormone receptors in human tissues: An immunahisLoehemical study. J. Clin. Endocrinol. Metab. 1996;81 (8): 3097-3102.
6. Leung K-C, HO KYK-. Stimulation of mitochondria] fatty acid oxidation by growth hormona in human fibroblasts. J.clin Endocrinol. Mctab. 1997. 82(12): 4208-4213
7. Martinez AJ, Paseual M, [.arraldc J. Acute effects of insülin-nke growth factor-1 (IGF-1J on bone protein synthesis in rats. Biochimıca et Biophsica Acta- 1994;] 199: 101-103.
8. Canalis E, Mcharty TL, Centrella M. The role of growth factors in skeletal remodeling. Endocrinol Metab. Clin. North. Am. 1989; 18(4); 903-917.
ERTAŞjKAYA, KİKİ, ERDOĞAN, AKÇAY
9. Kassem M. Cellular and molecular effects of growth hormone and estrogen on human bone cells.APMlS. (Suppl.) 1997;!05(7I):^30
10. tievhaug A, Mjaaiand M. Growth hormone and surgery. Horm.Re&. 1993; 40(1-3): 99-101 Reiew.
1 L Belcher CR, Ellis H. Somatotropin and wound healing after injury, J. Clin.Endocnnol. Mctab. 1990; 70(4): 939-943.
12. Waddel HK, Calton WC, Steinberg RS , Martındale RG, The Adverse effect of octrcotid on wound heaîıng in rats. Am.Surg. 1997; 63(5): 446-449.
13. Hak B, Jorgcnson HP, Andreassen IT. Dose reponse of growth hormone on fracture healing in the rat, Acta.Orthop.Scand. 1990; 62(1): 54-57.
14. Lane JM Sandhu S. Current.approaches to experimental bone grafting Orthop. Clin. NorthAm, 1987:18: 213-225.
15. Heiple KG, Goldberg VM, Powell AE, Bos GD, Zika J. Biology of cancellous bone grafts. Orthop. Clin. North. Am. 1987.Apr. t 18(2): 179-185.
16. Baktır A, Hoplamaz Ö, Karakaş ES, Türk CY. Kallotasis metoduyla ekslremite uzatılması, Acta. Orthop. Traum. 1991;25:46-53.
17. Nielsen HM, Bak B, Jorgensen HP, Andreassen TT. Growth hormone promotes healing of tibial fractures in the rat Acta. Orthop, Scand.1991; 62(3): 244-247
. 18. Bak Bv Jorgensen PH, Andreassen TT Increased mechanical strength of healing rat tibial fractures treated •with biosynthetic human growth hormone. Bone, 1990; 11: 233-239.
f9. Mosekildc L, Bak B. The Effects of Growth hormone on fracture healing in rats: A. histological description . Bone 1993; 14: 19-27.
20. Knox J. Demling R , Wilmore Dv Sarraf P, Santos A. Increased survival after major thermal injury; the effect of growth hormone therapy in adults. J Traum, 1995; 39(3): 526-532.
10
Atatürk Üniv.Diş Hek.Fak.Derg. Cilt: 14, Sayı 2, Sayfa:!-!] ,2004
ERTAŞ. KAYA, KİKİ, ERDOĞAN, AKÇAY
21. Hemdon DN, Hawkins K, Nguyen TT, PİCJTC E, Cox R, Barrow RE. Characterization of growth hormone enhanced donor site healing in patients with large cutaneus Bums. Ann. Surg. 1995; 221(6): 649-659.
22. Klein GL, Woir SE, Langman CB, Rosen C, Mohan S, Keenan BS, Malm S, Steffen C, Nicola M, Sailer DE, Heriidon DN. Effect of therapy with recombinant human growth hormone on insulin-like growth factor system components and serum level of biochemical markers of bone formation in children after severe burn injury. J. Clin.Endocrinol Metab. 1998; 83(1): 21-24.
23- Carpenter JE, Hipp JA, Gerhart NT, Rudman CG, Hayes WC.Tnppel SB. Failure of fracture-healing in a rabbit model. J.Bone Joint Surg (Am)-I992 Apr;l54 (15):1019-23.Rcview
24. Rasmussen LH, Garbargch C, Sebuppan DJvIcc D, Horslev-Pederson. K, Gattrup F, Stccnfos H. Dose response profiles of human growth hormone in subcuianens wound chambers in rats. Hur, J. Surg. 1995; 161: 157-162.
25. Bollcrslcv J, Moller J, Thomas S, Djoselanel O, Christiansen JS. Dose-dependent effects of recombinant human growth hormone on biochemical markers of bane and collagen metabolism in adult growth hormone deficiency. Eur. J. Endocrinol J996; 135: 666-71.
26. Ham son LE, Port JL, Hochw aid S. B1 urn berg D, Burt M. Perioperative growth hormone improves wound healing and immunologic function in rats receiving adriamydn J.Surg.Res 1995; 58: 646-650
27. Freeth JS, Ayhng RM, Whalmorc AJ Towner P, Price DA, Norman MR, Clayton PE. Human skin fibroblasts as a model of growth hormone (GH) action in GH receptor-positive Icon's syndrnome, J. Endocrinol. 1997; 138(1): 55-61.
28. Skottner A, Arrhenius- Nynberg V, Kange M, Fryklund L. Anabolic and tissue repair functions of recombinant insulin-like growth factor f. Acta Pcdiatr.Scand. (Suppl.) 1990; 367: 63-6.

Thank you for copying data from http://www.arastirmax.com