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AGRESİF PERİODONTİTİS Lİ BİREYLERDE NÜKLEER FAKTÖR KAPPA B AKTİVASYONUN İMMUN OHİSTOKİMYASAL OLARAK İNCELENMESİ: STEREOLOJİK ÇALIŞMA

IMMUNOHISTOCHEMICAL EXAMINATION OF NUCLEAR FACTOR KAPA B ACTIVATION IN INDIVIDUALS WITH AGGRESSIVE PERIODONTITIS: STEREOLOGIC STUDY

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
he purpose of this study was immunohistochemical and stereologic investigation of nuclear and cytoplasmic expression of p50 and p65 subunits of Nuclear Factor kappa B (NF-KB) and the cytoplasmic expression of Inhibitor kappa B (IKB), the inhibitor of NF-KB, in the gingival tissues of subjects with aggressive periodontitis. The study population was consisted of 30 individuals (15 aggressive periodontitis and 15 control) which were referred with different complaints to Ataturk University, Faculty of Dentistry between 2006 and 2007 years. 1 to 2 mm of gingival tissue was biopsied from the buccal side of the teeth of all individuals. Tissues were immediately transferred to 10% neutral buffered formalin and were then sent to immunohistochemical staining. The sections were examined under electron microscope and the numerical values of the stained cells were computed using stereologic method. One-way analysis of variance (ANOVA) and Multiple Range least significant difference (LSD) was used for intergroup comparison (p=0.05). According to the findings of this study, statistically significant differences were found in the number of cytoplasmic and nuclear positive stained cells with p65 and p50 in aggressive periodontitis group compared to control group (p<0.01). However, statistically significant positive correlations were found between laboratory findings and clinical scores (probing depth and clinical attachment level) in aggressive periodontitis group. In conclusion, it was found that NF-KB activation was highly important in patients with aggressive periodontitis. The present study forms the basis for future strategies that will generate specific inhibitors for using NF-KB inhibition to prevent the progressing of periodontitis.
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Abstract (Original Language): 
Bu çalışmanın amacı; agresif periodontitisli bireylerin diş eti dokularında Nükleer Faktör kappa B (NF-KB) proteinlerinden p50 ve p65 alt ünitelerinin çekirdek ve sitoplazmik ekspresyonunun ve NF-KB inhibitörü olan İnhibitör kappa B'nin (IKB) sitoplazmik ekspresyonunun immunohistokimyasal ve stereolojik olarak araştırılmasıdır. Çalışma popülasyonunu, Atatürk Üniversitesi Diş Hekimliği Fakültesine 2006-2007 yılları arasında değişik nedenlerle müracaat eden 30 birey (15'i agresif periodontitisli ve 15'i periodontal olarak sağlıklı) oluşturdu. Tüm bireylerden lokal anestezi altında dişlerin bukkal dişeti bölgesinden biyopsi ile 1-2 mm diş eti dokusu alındı. Alınan dişeti dokuları hemen % 101uk nötral tamponlu formalin içerisine konuldu ve sonra immunohistokimyasal boyamaya gönderildi. Elde edilen kesitler elektron mikroskobu altında incelendi ve stereolojik yöntem kullanılarak boyanan hücre sayısal değerleri hesaplandı. Grupların birbirleri ile karşılaştırmalarında Tek Yönlü Varyans Analizi ve Çoklu karşılaştırmalı en küçük önemli fark (LSD) testi (p=0.05) kullanıldı. Çalışma sonuçlarına göre p50 ve p65 ile hem çekirdek hem de sitoplazmada pozitif boyanan hücre sayısı açısından agresif periodontitis grubu ile kontrol grubu arasında istatistiksel olarak anlamlı fark bulundu (p<0.01). Bunun yanı sıra agresif periodontitisli grupta tüm immunohistokimyasal bulgular ile klinik skorlar (Sondalanabilir cep derinliği ve klinik ataşman seviyesi) arasında istatistiksel olarak anlamlı pozitif korelasyonlar tespit edildi. Sonuç olarak, NF-KB aktivasyonu agresif periodontitisli bireylerde oldukça anlamlı bulunmuştur. Bu çalışma periodontitisin ilerleyişini önlemek için NFKB inhibisyonunda kullanılmak üzere spesifik inhibitörlerin üretileceği gelecek stratejilere temel oluşturmaktadır.
FULL TEXT (PDF): 
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  • 3
84-91
  • Turkish

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