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ATATÜRK ÜNİVERSİTESİ DİŞ HEKİMLİĞİ FAKÜLTESİNE BAŞVURAN BİREYLERİN AEROBİK BAKTERİYEL FLORASININ MİKROBİYAL TEŞHİS SİSTEMİ KULLANILARAK SAPTANMASI

IDENTIFICATION OF AEROBIC BACTERIAL FLORA IN SALIVA OF SUBJECTS WHO APPLY TO THE FACULTY OF DENTISTRY IN ATATÜRK UNIVERSITY BY USING MICROBIAL IDENTIFICATION SYSTEM

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Introduction: The aim of the present study was to identify the aerobic bacterial flora in the saliva of patient who apply to The Faculty of Dentistry in Atatürk University. Methods: Unstimulated saliva samples were collected from 20 healthy subjects with Decayed Missed Filled Teeth (DMFT) index values of between 9-15. Total bacterial flora was recovered from saliva samples on two non-selective media, Nutrient Agar and Sensitive Agar. Isolated bacterial organisms were identified based on fatty acid methyl ester (FAME) analysis using the Microbial Identification System (MIS; MIDI Inc., Newark, Del.). Results: A total of 75 bacterial strains were isolated. Of these, 67 were identified as belonging to 34 species of 16 genera, while the remaining strains were unidentified. The most abundant bacterial genus was Streptococcus, followed by Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helicobacter, Actinomadura, Kocuria, Pseudomonas, and Cellulomonas. To our knowledge, this is the first report in the literature of the identification of the bacterial species Xanthobacter agilis, Actinomadura yumaensis and Psychrobacter-phenylpyruvicus in oral flora. Conclusions: This is the first study to utilize MIS for the identification of aerobic bacteria in saliva. Our results suggest that MIS is an accurate, efficient and relatively rapid method for identifying bacteria in saliva; however, the MIS databases, particularly those of oral bacterial flora, need to be improved.
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Abstract (Original Language): 
Bu çalışmanın amacı, Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran hastaların tükürük aerobik bakteri florasını tanımlamaktır. Yöntem: DMFT indeksi 9-15 arasındaki 20 sağlıklı bireyin sitümüle edilmemiş tükürükleri toplandı.Toplam bakteri florası iki seçici olmayan medya, Nutrient Agar ve Sensitive Agar üzerindeki tükürük örneklerinden toplanmıştır. Izole bakteriyel organizmalar, fatty acid methyl ester (FAME) analizine dayanan Microbial Identification System (MIS; MIDI Inc., Newark, Del.) kullanılarak sınıflandırıldı . Sonuçlar. 75 bakteri suş izole edildi. Bunlardan, 67’sinin , 16 cins ve 34 türe ait olduğu saptanırken Kalan suşlar tanımlanamadı. En çok bulunan bakteri Streptococcus iken takip edenler Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helikobakter, Actinomadura, Kocuria, Pseudomonas ve Cellulomonas oldu. Bilgilerimize dayanarak bu çalışma, bakteri türlerinden Xanthobacter Agilis, Actinomadura yumaensis ve Psychrobacter-phenylpyruvicus’u ağız florasında tanımlayan ilk literatürdür. Sonuç: Bu Çalışma;tükürük aerobik bakterilerinin tespiti için MIS’den yararlanılan ilk çalışmadır. Bizim sonuçlarımız MIS’in tükürük bakterinin tespiti için verimli ve hızlı ve doğru bir yöntem olduğunu desteklemektedir; ancak MIS veritabanlarının özellikle ağız bakteri florası olanlarının geliştirilmesi gerekmektedir.
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  • 1
26-30
  • Turkish

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