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ASETAMİNOFENİN YENİ-DOĞAN VE ERİŞKİN SIÇANLARDA KARŞILAŞTIRMALI HEPATOTOKSÎK ETKİSİ: IŞIK VE ELEKTRON MİKROSKOBİK BİR ÇALIŞMA

ACETAMINOPHEN-INDUCED HEPATOTOXICITV IN NEW-BORN HATS COMPARED TO ADULTS: A LIGHT AND ELECTRON MICROSCOPIC STUDY

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 1996

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Twelve adult and twelve new-bom male Wistar albino rats were used in this study. In each group, five rats received 300 mg/kg and five 500 mg/kg doses of acetaminophen intraperitoneaily. After 8 hours animals were killed with decapitation. There was small number of necrotic cells in the livers of 300 mg/kg acetaminophen administered new-born rats whereas 500 mg/kg acetaminophen administered rats developed mild hepatic necrosis. In adult rats received 300 mg/kg of acetaminophen there was moderate and received 500 mg/kg of acetaminophen there was severe hepatic necrosis. So, the hepatotoxicity of acetaminophen was age and dose-dependent. Ultraslruciurally, the liver of the new-bom rats exposed to toxic doses of acetaminophen contained areas of prominent intercellular spaces, In both groups the hepatocytes were swollen and had ruptured cytoplasmic membranes and disrupted internal structures. Membranes of endoplasmic reticulum were disrupted and endoplasmic reticulum was fragmented. Membranous debris was present in the intercellular spaces. In the 500 mg/kg acetaminophen group cytoplasmic membrane damage and the cell swelling were greatly increased and ioss of cytosol was observed. To the best of our knowledge, this is the first ultrastrcictural study performed to investigate the alterations caused by acetaminophen in new-born liver. We suggest that acetaminophen causes hepatic necrosis and irreversible changes at an earlier postnatal age within a shorter time and by administering lower toxic doses than that of previously reported
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Abstract (Original Language): 
Bu çalışmada oniki erişkin ve oniki yenidoğan Wistar albino türü sıçan kullanıldı. Her gruptan beş havana. 300 m g/kg ve be? hayvana 500 m g/kg dozunda asetaoÜDofcn mraperitoncal otarak uygulandı. Sekizinci aratie hayvanlar dekapite edildiler. 300 mg/kg a&etamrnofen uygulanmış yeni-doğan amaçların kuracağerieriJKİe az sayıda nekrotik hücre izlenirken, 500 mg/kg uygulanmış, sıçanların karaciğerlerinde az sayıda KHOtıtik httere izlenirken, 500 mg/kg uygulanmış olanlarda llafif hc|aak nekroz gödendi. 300 mg/kg asetaminophen uygyjonınu^ erişkin sıçanlarda orta derecede ve 500 m g/kg »f^aniflçfen uygulanmış olanlarda ise ciddi hepatik nekroz İzlendi. Böylece asetaminofen hepatotoksisitesinin yaşa ve -cfena bağh olduğu güdendi. Uttrasüliktürcl alarak toksik •dwtiffBa asetaminofen uygulanmış yem-doğan sıçanların tatTM^^rtprüıde belirgin in£ersellliler alanlar İzlendi. Her iM: - <£a : grubunda hepatositlerde şişme, sitoplazmik mejşhCTniafda parçalanma ve hücre içi yapılarda dağılma ^4sMfB£, Eiıdoplazma reli kulumu parçalanmış ve ifcHWhranlari dağılmıştı. Membran artıkları intersellüler UMuKb izleniyordu. 500 mg/kg asetaminophen •iyyajwirnt; grupta sitoplazmik membran hasan ve hücre wttpit: ol<kıkça belirgindi ve sitozol kaybı izlendi. - tjbsfstar aragrjnrjalanmıza göre çalışmamız yeni-doğan $JBtN£fade tok&k dozda asetaminofenin karaciğerde âtaftSttcfcıgU denklikleri incelemek için yapdmık ilk e^fcktmtı nufroskopik çalışmadır. Asetaminofenin daha Şbcşp rapor edilenden daha erken bâr postnatal dönemde, danİ Jj^ffifc dozlarda ve daha kısa sürede hepatik nekroza WjHBwmibl değfikliklere neden olduğu kanısındayız
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  • 2
44-50
  • Turkish

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