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Homalothecium sericeum özütlerinin zamana bağlı olarak glioma hücrelerinin çoğalması üzerindeki etkileri

Time dependent cytotoxic role of Homalothecium sericeum extracts on glioma

Journal Name:

  • Biyolojij Çeşitlilik ve Koruma

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Bryophytes have been used as medicinal plants for more than 400 years in China, Europe and North America. There is also evidence confirming the antibiotic and anticancer activity of Bryophytes against, prokaryotes, fungi and different cancer cells. The purpose of the current study was to investigate cytotoxic property of Homalothecium sericeum (hedw.) schimp., which is a bryophyte, extracts on rat glioma (C6) cells for 48 hrs, in vitro. We first collected two different (acetone and A) extracts from H. sericeum by two different extraction processes. C6 cells were seeded in 96 well plates (2x104 cells/well) and incubated for 24 hrs. Following this incubation period, the medium was replaced with only medium (control) or medium with extracts at concentrations of 0.17, 1.7, 17, 85 or 170 Lig/mL for 48 hrs. Cytotoxicity was determined by using 3-(4,5-dimethylthiazol-2yl)- 2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Acetone extract of H. sericeum at 0.17, 1.7 and 17 Lig/mL concentrations did not change the survival rate of C6, but 85 and 170 Lig/mL inhibited about 16 % and 36 % after 48 hr (p<0.001), respectively. Extract A at concentration of 0.17 Lig/mL did not also affect C6 viability, but 1.7, 17 (p<0.01), 85 and 170 (p<0.001) Lig/mL decreased C6 cell viability by 6, 8, 24 and 33 % for 48 hr, respectively. Acetone and A extracts of H. sericeum showed a moderate but similar dose dependent cytotoxicity on C6. Further studies are needed to clarify the content of these extracts.
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Abstract (Original Language): 
Bryofıtler 400 yıldan fazla bir süredir Çin, Avrupa ve Kuzey Amerika'da bitkisel ilaç olarak kullanılmaktadır. Bryofitlerin prokaryot, mantar türleri üzerinde antibiyotik ve farklı kanser hücreleri üzerinde antikanser etkinlik gösterdiği konusunda veriler bulunmaktadır. Bu çalışmanın amacı, bir Bryofit olan Homalothecium sericeum 'un sıçan glioma (C6) hücreleri üzerinde 48 saatlik sitotoksik etkinliğini araştırmaktır. İlk olarak, iki farklı özüt elde etme yöntemiyle aseton ve A özütlerini elde ettik. C6 hücreleri 96 kuyucuklu kültür kaplarına ekilerek (2x104 hücre/kuyucuk) 24 saat inkübe edildi. İnkübasyon süresinin ardından kuyucuklara özütlerin 0.17, 1.7, 17, 85 ve 170 Lig/mL konsantrasyonları eklenerek 48 saat muamele edildi. Sitotoksisite, MTT [3-(4,5-dimetiltiazol-2yl)- 2,5-difeniltetrazolyum bromid] yöntemi ile belirlendi. 48 saat sonunda aseton özütünün 0.17, 1.7 ve 17 Lig/mL konsantrasyonları C6 hücre canlılığını değiştirmezken, 85 ve 170 Lg/mL konsantrasyonları hücre canlılığını sırası ile %16 ve %36 oranında azalttı (p<0.001). A özütünün 0.17 Lig/mL konsantrasyonu hücre canlılığını 48 saat sonunda değiştirmezken, 1.7, 17 (p<0.01), 85 and 170 (p<0.001) Lig/mL konsantrasyonları sırası ile % 6, 8, 24 ve 33 oranında azaltmıştır. H. sericeum'un aseton ve A özütü glioma hücreleri üzerinde orta ve benzer bir şekilde doza bağımlı sitotoksik etki göstermiştir. Bu özütlerin içeriği hakkında yapılacak daha kapsamlı çalışmalara ihtiyaç duyulmaktadır.
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