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Ilaç Hammaddesi Olarak Sentezlenen Elektrofilik ve Nonelektrofilik Fenolik Schiff Bazlarının Umu Test Sistemi ile Genotoksik Aktivitesinin Araştırılması

Journal Name:

  • Çankaya University Journal of Science and Engineering

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
In this work, we investigated, through a short-term genotoxicity experiment, the umu-test, the mutagenic activities of a group of phenolic Schiff bases, namely Material A 2-(N-phenyliminomethyl)-phenol, Material B 2-(N-p-bromophenyliminomethyl)-phenol, Material C 2-(N-p-methylphenyliminomethyl)-phenol and Material D 2-(N-p-anilinephenyl iminomethyl)-phenol, the substances which we synthesized, in the chemistry laboratories of our university, to form the raw materials of medicines. The umu-test method is a colorimetric bacterial test system and has been developed on the basis of SOS response induced by DNA damage. Materials A, B, C and D and the experimental ma¬terials prepared in 5 different dose levels by dissolving these materials in DMSO have not increased the galactosidase enzyme activity. When calculated with respect to the enzyme unit calculation formula, results seemed to be not larger than the unit value of the positive mutagenic material 4NQO. Results have also been evaluated statistically.
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Abstract (Original Language): 
Bu çalışmamızda Üniversitemiz Kimya Bölümü laboratuarlarında sentez ettiğimiz ve ilac hammaddesi olarak kullanılmasını amaçladığımız fenolik Schiff bazlarının bir grubunu olusturan A maddesi 2-(N-feniliminometil)-fenol, B maddesi 2-(N-p-bromofeniliminometil)-fenol, C maddesi 2-(N-p-metilfeniliminometil)-fenol, D maddesi 2-(N-p-anilinfeniliminometil)-fenol'ü mutajenik aktiviteleri acısından kısa zamanlı geno-toksisite testlerinden olan umu-test sistemi ile arastırdık. Umu-test yontemi kalometrik bakteriyel bir test sistemidir ve DNA hasarı ile indüklenen SOS cevabı esas alınarak ge-listirilmistir. A, B, C, D maddeleri ve bunların DMSO'de cozülerek hazırlanan 5 farklı doz seviyelerindeki deney materyalleri galaktozidaz enzim aktivitesini artırmamıçstır. Sonuçclar enzim ünite hesaplama formülüne gore hesaplanınca pozitif mutajen olan 4NQO mad¬desinin uünite degğerinden fazla çcıkmamıçstır. Sonuçclar ayrıca istatistiki olarak da değgerlen-dirilmiçstir.
FULL TEXT (PDF): 
PDF icon arastirmax-ilac-hammaddesi-olarak-sentezlenen-elektrofilik-nonelektrofilik-fenolik-schiff-bazlarinin-umu-test-sistemi-ile-genotoksik-aktivitesinin-arastirilmasi.pdf
  • 2
75-86
  • Turkish

REFERENCES

References: 

[1] H. Bagci, J. H. Stuy, Bromouracil-induced mutagenesis in a mismatch-repair-deficient strain of Haemophilus influenzae, Mutat. Res. 73 (1994), 15-19.
[2] P. Quillardet and M. Hofnung, The SOS Chromotest, a colorimetric bacterial assay for geno-toxins: procedures, Mutat. Res. 147 (1985), 65-78.
[3] D. Forman, Ames, the Ames test, and the causes of cancer, BMJ 303 (1991), 428-429.
[4] B. N. Ames, Mutagenesis and carcinogenesis: Endogenous and exogenous factors, Environ. Mol. Mutagen. 14 (1989), 66-77.
[5] P. Einistoü, M. Watanabe, M. Jr. Ishidate, T. Nohmi, Mutagenicity of 30 chemicals in Salmonella typhimurium strains possessing different nitroreductase or O-acetyltransferase activities, Mutat. Res. 259 (1991), 95-102.
[6] R. Vikse, A. Knapstad, L. Klungs0yr and S. Grivas, Mutagenic activity of the methyl and phenyl derivatives of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoxaline (IQx) in the Ames test, Mutat. Res. 298 (1993), 207-214.
[7] M. Manneberg, W. Lahm and M. Fountoulakis, Quantification of cysteine residues following oxidation to cysteic acid in the presence of sodium azide, Anal. Biochem. 231 (1995), 349-353.
[8] J. S. Fisherman, B. L. Osborn, H. G. Chun, J. Plowman, A. C. Smith, M. C. Christian, D. S. Zaharko and R. H. Shoemaker, Chloroquinoxaline sulfonamide: A sulfanilamide antitumor agent entering clinical trials, Invest. New Drugs 11 (1993), 1-9.
[9] N. S. Habib and S. A. el-Hawash, Synthesis and antimicrobial testing of thiazolinyl-, thiazolidinonyl-quinoxalines and 1,2,4-triazolo[4,3-a]quinoxalines, Pharmazie 52 (1997), 594¬598.
[10] J. B. Sutherland, F. E. Evans, J. P. Freeman and A. J. Williams, Biotransformation of quinox-aline by Streptomyces badius, Lett. Appl. Microbiol. 22 (1996), 199-201.
[11] Y. Oda, H. Yamazaki, M. Watanabe, T. Nohmi and T. Shimada, Highly sensitive umu test system for the detection of mutagenic nitroarenes in Salmonella typhimurium NM3009 having high O-acetyltransferase and nitroreductase activities, Environ. Mol. Mutagen. 21 (1993),
357-364.
[12] Y. Oda, H. Yamazaki and T. Shimada, Role of human N-acetyltransferases, NAT1 or NAT2, in genotoxicity of nitroarenes and aromatic amines in Salmonella typhimurium NM6001 and NM6002, Carcinogenesis 20 (1999), 1079-1083.
86
Çeyhan et al.
[13] Y. Oda, H. Yamazaki, M. Watanabe, T. Nohmi and T. Shimada, Development of high sen¬sitive umu test system: rapid detection of genotoxicity of promutagenic aromatic amines by Salmonella typhimurium strain NM2009 possessing high O-acetyltransferase activity, Mutat. Res. 334 (1995), 145-156.
[14] G. Reifferscheid and J. Heil, Validation of the SOS/umu test using test results of 486 chemicals and comparison with the Ames test and carcinogenicity data, Mutat. Res. 369 (1996), 129¬145.
[15] D. W. Hein, M. Hirata, I. B. Glowinski and W. W. Weber, Biochemical evidence for the coexistence of monomorphic and polymorphic N-acetyltransferase activities on a common protein in rabbit liver, J. Pharmacol. Exp. Ther. 220 (1982), 1-7.
[16] O. H. Lowry, N. J. Rosenbrough, A. L. Farr and R. J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951), 265-275.
[17] K. T. Bogen, Applicability of alternative models of revertant variance to Ames-test data for
121 mutagenic carcinogens, Mutat. Res. 322 (1994), 265-273.
[18] L. Dalgaard, R. K. Hjortkjaer, B. Regnier and L. Nordholm, Pharmacokinetics of the neuro-protective glutamate antagonist NBQX (6-nitro-7-sulfamoyl-benzo(f)quinoxaline-2,3-dione) in mice, rats, and dogs. Interactions with probenecid, Drug Metab. Dispos. 22 (1994), 289-293.
[19] C. Sengstag C and F. E. Würgler, DNA recombination induced by aflatoxin B1 activated by cytochrome P450 1A enzymes, Mol. Carcinog. 11 (1994), 227-235.
[20] N. Shibuya, T. Ohta, H. Nakadaira, H. Mano, K. Endoh and M. Yamamoto, Mutagenicity of activated carbon adsorbate of drinking water in the Ames assay, Tohoku J. Exp. Med. 171 (1993), 89-95.
[21] L. D. Claxton, J. Creason, B. Leroux, E. Agurell, S. Bagley, D. W. Bryant, Y. A. Courtois, G. Douglas, C. B. Clare, S. Goto, P. Quillardet, D. R. Jagannath, K. Kataoka, G. Mohn, P.
A. Nielsen, T. Ong, T. C. Pederson, H. Shimizu, L. Nylund, H. Tokiwa, G. J. Vink, Y. Wang
and D. Warshawsky, Results of the IPCS collaborative study on complex mixtures, Mutat.
Res. 276 (1992), 23-32.
[22] S. Monarca, R. Pasquini and P. Arcaleni, Detection of mutagens in unconcentrated and con¬centrated drinking water supplies before and after treatment using a microscale fluctuation test, Chemosphere 14 (1985), 1069-1080.
[23] M. Font, A. Monge, E. Alvarez, A. Cuartero, M. J. Losa, M. J. Fidalgo, C. SanMartın, E. Nadal, I. Ruiz, I. Merino, J. J. Martmez-Irujo, E. Alberdi, E. Santiago, I. Prieto, J. J. Lasarte, P. Sarobe and F. Borras, Synthesis and evaluation of new Reissert analogs as HIV-1 reverse transcriptase inhibitors. 1. Quinoline and quinoxaline derivatives, Drug Des. Discov.
14 (1997), 305-332.

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