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Wistar Ratlarınd a (Rattus rattus norvegicus) Kalitatif ve Kantitatif Protein Yetersizliklerinin İskelet Kası Malondialdehit Düzeylerine Etkileri

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2002

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In this study 41 male Wistar rats (Rattus rattus norvegicus) were used. Animals were divided into three groups and were fed eather with a rat chow diet (n=7, controlgroup) or with a semisynthetic diet containing %20 gelatine as protein source (n=17, gelatin group) and no protein (n=17, N-free group). At the end of experiment animals were euthanized, approximately 1 g skeletal muscle samples were taken and MDA levels per gram of muscle tissue were determined. Tissue MDA levels of the rats fed gelatin-containing diet were lower than the controls and animals fed with N-free diet. However, the differences in mean MDA levels among groups were not confirmed statisticcaly. It was concluded that this may be the result of metabolic adaptation processes of organismus to severely protein malnutrition conditions.
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Abstract (Original Language): 
Çalışmada 41 adet yaklaşık 2.5 aylık erkek Wistar ratı (Rattus rattus norvegicus) kullanıldı. Hayvanlar rat yetiştirme yemi (n=7, kontrol), jelatin içeren (n=17) ve protein içermeyen (n=17) yemlerle 35 gün beslendiler. Deney sonunda hayvanlar ötenazi edilerek yaklaşık 1 g kadar iskelet kası örneği alındı ve gram kas dokusu için malondialdehit (MDA) düzeyleri belirlendi. Jelatinle beslenen grupta iskelet kası MDA değerleri kontrol grubu ve N-free diyetle beslenen hayvanlarınkinden daha düşüktü. Ancak gruplar arasında fark istatistiksel olarak onaylanmadı. Bu durumun organizmanın ileri düzeydeki protein yetmezliklerine gösterdiği metabolik uyuma bağlı olduğu düşünülmektedir.
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REFERENCES

References: 

[1] Lane, H.W., Schulz, L.O.: Nutritional questions relevant to space flight. Ann Rev Nutr 12: 257-278,1992.
[2] Stein, T.P.: Nutrition in the space station era. Nutr Res Rev 14: 87-117, 2001.
[3] Grounds, M.D.: Reasons for the degeneration of ageing skeletal muscle: a central
role for IGF-1 signalling. Biogerontology 3:19-24, 2002.
[4] Preedy,V. R., Adachi, J., Asano, M., Koll, M., Mantle, D., Niemela, O., Parkkila, S.,
Paice, A. G., Peters, T., Rajendram, R., Seitz, H., Ueno, Y, Worrall, S.: Indices of
damage and preventive studies. Free Radical Biol Med 32: 683-687, 2002.
[5] Muramatsu, T., Okumura, J-I.: Effect of dietary methionine and arginine on uric acid
excretion of cocks fed a protein-free diet. J Nutr 109: 1057-62,1979.
[6] Koch, M.: Die Harnstoff- und Kreatininausscheidung der Albinoratte bei N-freier
Ernâhrung.
Inaugural-Dissertatio
n zur Erlangung des Doktorgrades beim Fachbereich
Veterinârmedizin der Justus-Liebig Universitât GieBen, 1983.
[7] Nau R.: Untersuchung zum N-stoffwechsel der Albinoratte. Inaugural-Dissertation zur Erlangung des Doktorgrades beim Fachbereich Veterinârmedizin der Justus-Liebig Universitât GieBen, 1988.
[8] Cao, G., Prior, R.L., Cutler, R.G., Yu, B.P.: Effect of dietary restriction on serum antioxidant capacity in rats, Arch Gerontol Geriatrics 25: 245-253,1997. [9] Maiti, S., Chatterjee, A. K.: Response of certain antioxidant enzymes to arsenite treatment in protein malnourished mice (BALB-C). Pathophysiol 5 (Suppl 1) 98,
1998.
[10] Maiti, S., Chatterjee, A. K.: Differential response of cellular antioxidant mechanism of liver and kidney to arsenic exposure and its relation to dietary protein deficiency. Environmental Toxicol Pharmacol 8: 227-235, 2000. [11] Fang, Y.Z., Yang, S., Wu, G.: Free radicals, antioxidants, and nutrition. Nutrition 18: 872- 879, 2002.
[12] Pamplona, R., Otin, M. P., Requena, J., Gredilla, R., Barja, G.: Oxidative, glycoxidative and lipoxidative damage to rat heart mitochondrial proteins is lower after 4 months of caloric restriction than in age-matched controls. Mechanisms Ageing Development 123: 1437- 1446, 2002.
[13] Shin, S. J.: Relation between oxidative damage and dietary protein: marginal protein level which modulates oxidative damage in mice with total body irradiation. Nutr Res 22: 1487-1495, 2002.
[14] Deneke, S. M., Gershoff, S. N., Fanburg, B.L.: Potentiation of oxygen toxicity in rats by dietary protein or amino acid deficiency. J Appl Physiol 54:147-51,1983. [15] Hum, S., Koski, K.G., Hoffer, L.J.: Varied protein intake alters glutathione metabolism in rats. J Nutr 122: 2010, 1992.
[16] Peuchant, E., Delmas-Beauvieux, M.C., Dubourg, L., Thomas, M. J., Perromat, A., Aparicio, M., Clerc, M., Combe, C.: Antioxidant effects of a supplemented very low protein diet in chronic renal failure. Free Radical Biol Med 22: 313-320,1997. [17] Balkaya, M.: Der EinfluB des Proteinmangels auf das weiBe Blutbild von Wistar-Ratten und Hâhnen der Rasse WeiBes Leghorn. Inaugural-Dissertation zur Erlangung des Doktorgrades beim Fachbereich Veterinârmedizin der Justus-Liebig Universitât GieBen, 1991.
[18] Balkaya, M.: The effects of fedding gelatin containing diet and following complate feeding on the peripheral white blood cells of the male and female Wistar rats. Tr. J. Vet Anim Sci 23: 417-429, 1999.
[19] Yoshioka, T., Kawada, K., Shimada, T., Mori M.: Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135: 372-376, 1979.
[20] Niemelâ, O.: Distribution of ethanol-induced protein adducts in vivo: Relationship to tissue injury. Free Radical Biol Med 31: 1533-1538, 2001. [21] Golden, M. H. N., Ramdath D.: Free radicals in the pathogenesis of kwashiorkor. In Proceedings of the X111 International Congress on Nutrition, Ed.
T. G. Taylor, N. K. Jenkins, pp. 597-598, 1985.
[22] Gong, X., Shang, F., Obin, M., Palmer, H., Scrofano, M.M., Jahngen-Hodge, J., Smith, D.E., Taylor, A., Antioxidant enzyme activities in lens, liver and kidney of calorie restricted Emory mice. Mechan Ageing Develop 99: 181-192, 1997. [23] Isong, E. U., Essien, E. U., Eka, U., Umoh, I. B.: Sex- and organ-specific toxicity in normal and malnourished rats fed thermoxidized palm oil. Food Chemical Toxicol 38, 997-1004, 2000.
[24] Rufeger, H.: Ein neuer Weg zur biologischen Proteinbewertung mit Hilfe einer N-Bilanzfunktion, zugleich ein Beitrag zur Theorie des N-Stoffwechsels. Habilitationsschrift, Fachbereich Veterinârmedşizin, Justus-Liebig Universitât
GieBen, 1970.
[25] Arshag, D., Mooradian, M.D., Deanna Reinacher, B.S., Jian Ping Li, M.S., Pinnas, J. L.: Malondialdehyde modification of proteins in vitro is enhanced in the presence of acetaldehyde. Nutr 17: 619-622, 2001.
[26] Tiku, M.L., Allison, G. T., Naik, K., Karry, S. K.: Malondialdehyde oxidation of cartilage collagen by chondrocytes. Osteoarthritis Cartilage11: 159-166, 2003.

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