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Diisobutyl Phthalate’ ın (DIBP) Sıçan Karaciğeri Üzerine Histopatolojik Etkileri

Histopathological effects of Diisobutyl Phthalate (DIBP) on Rat Liver

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DOI: 
http://dx.doi.org/10.17776/csj.49560

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Abstract (2. Language): 
The aim of this study is to investigate the histopathological effects on the liver tissues of widely used as plasticizer Diisobutyl phthalate (DIBP). In this study Wistar albino (n=10) rats were used. Three study groups were created: an experimental group, a control group fed with corn oil and a control group. The experimental group were administered by gavaj daily with 3 different dosages (0.25 - 0.5 - 1 ml/kg/day) of DIBP mixed with corn oil for 28 days. The liver tissue sections after rutin histological preparation processes were examined and photographed using an Olympus BX51 light microscope. It was determined from the analyses that there was no significant histological difference between the control and the corn-oil fed control group. The group which was administered DIBP displayed deterioration in lobulation, focal hepatocellular necrosis, oedema in hepatic artery and vena centralis, decrease in glycogen and distortion of nucleus shape in relation to the dosage of DIBP they received. As a result, it has been determined that DIBP is a hepatotoxic substance and has been found to cause irreversible serious histopathological changes in liver tissue.
Abstract (Original Language): 
Bu çalışmada, plastikleştirici olarak yaygın kullanılan fitalatlardan Diisobutyl phthalate’ın (DIBP) karaciğer dokusu üzerine etkilerinin histopatolojik yönden belirlenmesi amaçlanmıştır. Araştırmada Wistar albino cinsi sıçanlar (n=10/grup) kullanılmıştır. Çalışma kontrol, mısır yağı verilen kontrol ve deney grubu olarak üçe ayrılmıştır. Deney grubu hayvanlara 28 gün boyunca her gün üç farklı dozda (0.25-0.5-1ml/kg/gün) DIBP mısır yağı ile karıştırılarak gavaj yolu ile verilmiştir. Deneyin sonunda kontrol ve deney gruplarına ait tüm hayvanlardan alınan karaciğer doku örnekleri rutin ışık mikroskop histolojik preparasyon işlemlerinden (fiksasyon, dehidrasyon, bloklama, kesit alma, boyama, kapatma) sonra ışık mikroskobunda (Olympus BX51) incelenip değerlendirilmiştir. Histolojik incelemelerde kontrol ve mısır yağı kontrol grupları arasında histolojik açıdan farklılık olmadığı görülmüştür. DIBP uygulama gruplarında ise doza bağlı olarak artış gösteren lobulasyonda bozulma, fokal hepatoselüler nekroz, hepatik arter ve merkezi venlerde ödem, kongesyon, sinuzoidlerde genişleme, hepatositlerde sitoplazmik eosinofili, vakuolizasyon, glikojende azalma ve nükleuslarda şekil değişimi belirlenmiştir. Sonuç olarak DIBP’ın hepatotoksik olduğu ve karaciğer dokusunda geri dönüşümü olmayan ciddi histopatolojik değişikliklere yol açtığı tespit edilmiştir.

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