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Farklı Gün Uygulamalarının Bazı Siyanobakterilerde Norharman Üretimi Üzerine Etkisi

Effects of Differential Time Applications on Some Cyanobacterial Norharman Production Rates

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2016
DOI: 
http://dx.doi.org/10.17776/csj.13491

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Cyanobacteria are an important class of bacteria by their metabolic activities for biology, ecology and economy. They contain series of secondary metabolites produced under negative stress conditions and providing specialized functions. One of the metabolites which is biologically active and can be used as drug for antibacterial and antitumor properties is norharman which has (9H-pyrido 3, 4-b) indole structure. In this study, water samples were collected from Yesilirmak river of Tokat province of Turkey and cyanobacteria were isolated under inverted microscope by micropipette and microinjection and were cultured for a month. Selection from cultures was done during predetermined time courses and produced norharman levels were determined by HPLC. At 16th day maximum norharman production was determined as 8.8167 and 0.712 μg/g from Chroococcus minitus and Anabaena oryzae respectively. Highest norharman production from Nostoc linckia determined as 1.191 μg/g at 20th day. Since Geitlerinema carotinosum began exponential growth phase faster than other strains the highest amount of norharman production was determined as 0.825 μg/g at 12th day.
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Abstract (Original Language): 
Siyanobakteriler, metabolizmalarından ötürü biyolojik, ekolojik ve ekonomik bakımdan önemli bakterilerdir. Özellikle çeşitli olumsuz şartlarda üretilmiş ve her biri özelleşmiş fonksiyonlara sahip bir dizi sekonder metabolit içerirler. Antibakteriyel, antikanser gibi biyolojik etkili ve ilaç olarak kullanılabilen metabolitlerden biri; 9H-Pyrido [3,4-b] indole yapısında Norharman’dır. Bu çalışmada Yeşilırmak Nehri (Tokat)’nden su örnekleri alınarak inverted mikroskop altında mikropipet ve mikro enjektör yardımıyla izole edilen siyanobakteriler ortalama bir aylık kültüre alınmıştır. Belirli zaman aralığında kültürlerden alınarak ürettikleri norharman miktarı HPLC ile tespit edilmiştir. Chroococcus minutus ve Anabaena oryzae’ de sırasıyla 8.8167, 0.712 μg/g norharman 16. günde maksimum olarak üretilmiştir. Nostoc linckia’da en fazla norharman üretimi 1.191 μg/g olup 20. gündedir. Geitlerinema carotinosum’da ise diğer türlere göre daha erken logaritmik faza girdiği 12. günde maksimum norharman metaboliti 0.825 μg/g olarak üretilmiştir.
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  • 4
398
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  • Turkish

REFERENCES

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