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EGCg İlavesi Sevofluran Uygulanan Ratların Böbreklerinde Oksidan ve Antioksidan Durumunu Düzenler

EGCg Supplementation Improves Oxidant and Antioxidant Status in Kidney of Rats Exposed to Sevoflurane

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Abstract (2. Language): 
Objectives: To investigate the possible effects of sevoflurane anesthesia and epigallocatechin 3- gallate (EGCg) on kidney tissue by evaluating the oxidant and antioxidant status in rats. Materials and Methods: Tree groups of animals were studied. Sevoflurane 3% (v/v) in air/O2 were administered to animals in group 1 (n = 7) and sevoflurane plus EGCg was administered in group 2 (n = 7). Seven animals were allocated to control group (group 3). Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in kidney tissue were studied. Histopathological changes were also assessed. Results: In group I, MDA, NO, SOD and GSH-Px activities were increased while decreased CAT activities occurred when sevoflurane was exposured compared with controls. Supplementation of EGCg (group 2) decreased the MDA (P<0.05), SOD (P<0.005) and GSH-Px activities whereas, the NO (P<0.005) and CAT (P<0.005) activities increase with EGCg supplementation compared with group 1. In histopathology, sevoflurane exposure group had severe degeneration with moderate cortical necrosis in kidney tissue. Administration EGCg with sevoflurane exposure decreased degeneration. Conclusion: The amount of lipid peroxidation and antioxidant enzyme levels were more increased in following sevoflurane administration. The administration of EGCg significantly protected kidney tissue. ©2008, Firat University, Medical Faculty
Abstract (Original Language): 
Amaç: Ratlarda böbrek dokusu üzerinde oksidan ve antioksidan durumu değerlendirerek sevofluran anestezisi ve epigallocatechin 3- gallate (EGCg)'ın muhtemel etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Ratlar 3 gruba ayrılarak çalışıldı. Grup 1 (n:7): Sevofluran, %3 (v/v) hava/O2 karışımı ile uygulandı. Grup 2 (n:7): Sevofluran + EGCg uygulandı. Kontrol grubu için ise yine 7 tane rat seçildi. Malondialdehid (MDA), nitrik oksit (NO), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) böbrek dokusunda çalışıldı. Ayrıca histopatolojik değişiklikler değerlendirildi. Bulgular: Grup 1'de MDA, NO, SOD ve GSH-Px aktiviteleri artmışken, CAT aktivitesi sevofluran uygulananlarda azalmıştır. EGCg'nin uygulaması (grup 2) MDA, SOD ve GSH-Px aktivitelerini azaltırken, NO ve CAT aktiviteleri grup 1 ile karşılaştırıldığında artmış olarak bulunmuştur. Histopatolojide sevofluran uygulanan grupta böbrek dokusunda orta derecede kortikal nekroz ve şiddetli dejenerasyonla karakterize değişimlerin oluştuğu saptandı. Sevofluran ile birlikte EGCg uygulamasının ise dejenerasyonu azalttığı bulunmuştur. Sonuç: Lipid peroksidasyonu ve antioksidan enzim düzeyleri sevofluran uygulamasını takiben fazla miktarda artmıştır. Ancak, EGCg uygulaması böbrek dokusunu önemli ölçüde korumaktadır. ©2008, Fırat Üniversitesi, Tıp Fakülte

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Kabul Tarihi: 10.08.2007

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