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Fasulye (Phaseolus Vulgaris L.) Çeliklerinde Ağırlık Değişimleri, Pigment ve Protein Miktarları Üzerine Asetilsalisilik Asit ve Tuz (NaCl) Uygulamasının Karşılıklı Etkileri

Interactive Effects of Acetylsalicylic Acid and Salt (NaCl) Application on Weight Changes,Pigment and Protein Amounts at Bean (Phaseolus Vulgaris L.) Shoots

Journal Name:

  • Gazi Eğitim Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

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Abstract (2. Language): 
The aims of this study were to investigate the interactive effects of 50 ppm acetylsalicylic acid (ASA) and 1 % NaCl on amount of protein and pigment, changes of wet-dry weight at shootstaken from one-week-old bean (Phaseolus vulgaris L.) seedlings. Experiment solutions applied to stem cut tips of seedlings via a closed set. According to the experimental results; from the wet weight increase point of view, no difference was, statistically, observed between shoots of control and 50 ppm ASA treated groups. However, wet weight lost ofshoots treated with 50 ppm + 1 % NaCl was found lower than that of 1 % NaCl treated ones. Because of little water content at shoots that subjected to salts stress, the dry subtance percent amount was found to be lower than the ASA treated shoots. Water contents of control (buffer), 50 ppm ASA, 50 ppm ASA + 1 % NaCl and 1 % NaCl groups were observed to be higher sequentially. Total pigment II and chlorophyll b levels were found as 50 ppm ASA>control>50 ppm ASA + 1 % NaCl>1% NaCl treated shoot groups. Total pigment I and chlorophyll a levels were found as control=50 ppm ASA>50 ppm ASA + 1 % NaCl=1% NaCl treated shoot groups. Carotenoid amount of control shoots was higher than that of the other groups. There was not any difference statistically in carotenoid amounts between other three groups. Total protein levels were found as control=50 ppm ASA>50 ppm ASA + 1 % NaCl>1% NaCl in shoots. As a result, opposite to the osmotic effect created by salt ASA application inhibited the wet weight lost, collapsing of chlorophyll b and total pigment II were decreased at certain levels in shoots. It has not affected carotenoid amount at an important level. In addition, it also decreased the protein collapsing at shoots in stress created by salt.
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Abstract (Original Language): 
Bu çalışmada, sera koşullarında yetiştirilen bir haftalık fasulye (Paseolus vulgaris L.) fidelerinden alınan çeliklerde ağırlık ve yaş-kuru ağırlık değişimi, pigment ve protein miktarıüzerine 50 ppm asetilsalisilik asit (ASA) ile % 1 NaCl ’nin karşılıklıetkileri araştırılmıştır. Deneme çözeltileri çeliklerin kesik gövde uçlarına kapalıbir sistem yoluyla uygulanmıştır. Elde ettiğimiz sonuçlara göre; yaşağırlık artışıbakımından 50 ppm ASA uygulanmışçelikler ile kontrol grubuna ait çelikler arasında istatistik açıdan fark gözlenmemiştir. Ancak 50 ppm ASA + % 1 NaCl uygulanmışçeliklerdeki yaş ağırlık kaybının % 1 NaCl uygulanmışçeliklere göre daha az olduğu gözlenmiştir. Tuz stresine maruz bırakılmışçeliklerin su içerikleri ASA uygulanmışçeliklere göre daha düşük çıktığıiçin, ASA uygulamasıkuru madde miktarının % olarak değerini düşürmüştür. Su içeriği miktarının gruplara göre ve sırasıyla çoktan aza doğru kontrol, 50 ppm ASA, 50 ppm ASA + % 1 NaCl, % 1 NaCl şeklinde olduğu gözlenmiştir. Çeliklerdeki klorofil b ve total pigment II miktarının gruplara göre ve sırasıyla çoktan aza doğru 50 ppm ASA >kontrol > 50 ppm ASA + % 1 NaCl >% 1 NaCl şeklinde olduğu gözlenmiştir. Klorofil a ve total pigment I miktarının gruplara göre ve sırasıyla çoktan aza doğru kontrol = 50 ppm ASA > 50 ppm ASA + % 1 NaCl = % 1 NaCl şeklinde olduğu gözlenmiştir. Çeliklerin karotenoid içerikleri ise sadece kontrol grubunda yüksek çıkmış, diğer gruplar arasında herhangi bir fark belirlenmemiştir. Çeliklerde total protein miktarlarının gruplara göre ve sırasıyla çoktan aza doğru kontrol = 50 ppm ASA > 50 ppm ASA + % 1 NaCl > % 1 NaCl şeklinde olduğu gözlenmiştir. Sonuç olarak, asetilsalisilik asit tuzun yarattığıosmotik etkiye karşı; çeliklerde yaşağırlık kaybını, klorofil b ve total pigment II yıkımınıbelirli oranlarda engellemiştir. Karotenoid miktarıüzerinde önemli bir etkide bulunmamıştır. Ayrıca çeliklerde strese bağlıolarak ortaya çıkan protein yıkımınıazaltmıştır.
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