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Bazı Kırmızıbiber Hatlarının Patates Y Virüs (Potato Virus Y, Pvy) Patotiplerine Reaksiyonları

Investıgatıon For Reactıons Of Peppers With Pvr Locı To Potato Virus Y (Pvy)

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DOI: 
http://dx.doi.org/10.13002/jafag900

Keywords (Original Language):

Abstract (2. Language): 
Reactions of 43 red pepper lines to PVY pathotypes, (0), (0,1), (0,1,2) were investigated in this study. PVY isolates LYE84, CAA16 and SON41P which arePVY pathotypes (0), (0,1) and (0,1,2) were multiplied in Nicotiana tabacum L. "Samsun" and the virus presence was confirmed ELISA tests. The relationship between pathotypes and pvr loci was biologically and serologically checked onto Yolo Wonder, Yolo Y, Florida VR2 and W4 pepper genotypes. PVY pathotypes were mechanically inoculated into red pepper lines. The virus multiplicaion and spread regarding with three pathotypes were tested by DAS-ELISA for three weeks after first symptom appearance on positive controls.PVY (0) caused leaf mosaic while PVY (0,1) induced deformation on leaf surface, downward leaf rolling, stem deformation and elongated color deformation on fruits. PVY (0,1,2) caused necrosis along the veins and defoliation afterwards. Three lines did not have any symptoms due to virus infection. According to results of serological tests, they can be accepted as resistant candidates to three of PVY pathotypes
Abstract (Original Language): 
Bu çalışmada, baharat yapımına uygun aday 43 kırmızıbiber hattının Patates Y virüsü (PVY)'nin (0), (0,1), ve (0,1,2) patotiplerine reaksiyonları araştırılmıştır. PVY’nin, LYE84 (0), CAA16 (0,1) ve SON41P (0,1,2) izolatları Nicotiana tabacum L. " Samsun " tütün çeşidi üzerinde çoğaltılmış ve virüsün varlığı PVY poliklonal antiserum kullanılarak DAS-ELISA testi ile doğrulanmıştır. Daha sonra patotiplerin pvr lokusuyla ilişkileri Yolo Wonder, Yolo Y, Florida VR2 ve W4 biber genotiplerinde biyolojik ve serolojik olarak kontrol edilmiştir. Denemeye alınan kırmızıbiber hatlarına, PVY patotipleri mekanik olarak inokule edilmiştir. Biber hatlarında PVY patotiplerinin çoğalması ve yayılması, pozitif kontrollerde ilk simptomları takiben DAS-ELISA testiyle üç hafta süreyle izlenmiştir. PVY (0) patotipi, bitki yapraklarında mozaik, PVY (0,1) patotipi, yaprak yüzeyinde deformasyon, yapraklarda içe kıvrılma, bitki gövdesinde şekil bozukluğu ve meyvede şeritler halinde renk açılması simptomları oluşturmuştur. PVY (0,1,2) patotipi, yaprak damarı çevresinde nekrozlar oluşturmuş ve yapraklar dökülmüştür. Üç kırmızıbiber hattında PVY patotiplerinden kaynaklı simptom meydana gelmemiştir. Serolojik testlerden alınan sonuçlara göre, bu üç hattın, PVY’nin üç patotipine dayanıklı aday hatlar olduğunu söylemek mümkündür.
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REFERENCES

References: 

Abak K (1982). Biberlerde kökboğazı yanıklığına
dayanıklılığın kalıtımı üzerinde araştırmalar.
(Doçentlik Tezi), Fen Bilimleri Enstitüsü, Ankara
Üniversitesi, Ankara.
Arli Sokmen M, Mennan H, Sevik M A and Ecevit O
(2005). Occurrence of Viruses in Field-grown Pepper
Crops and Some of Their Reservoir Weed Hosts in
Samsun, Turkey. Phytoparasitica, 33 (4): 347-358.
72
KARATAŞ ve ark./ JAFAG (2017) 34 (2), 65-73
Boonham N and Barker I (1998). Strain Spesific
Recombinant Antibodiesto Potato Virus Y potytvirus.
Journal of Virological Methods, 74: 193–199.
Bostan H ve Dursun A (2002). Kemaliye ve Yusufeli
İlçelerindeki Biber (Capsicum annuum L.) Üretim
Alanlarındaki Bazı Virüs Hastalıklarının Belirlenmesi.
Atatürk Üniv. Zir. Fak. Dergisi, 33: 391-392.
Buzkan N, Demir M, Öztekin V, Mart C, Çağlar BK and
Yılmaz MA (2006). Evaluation of the status of
Capsicum viruses in the main growing regions of
Turkey. EPPO Bulletin, 36: 15-19.
Caranta C, Thabuis A and Palloix A (1999). Development
of a CAPS marker for the Pvr4 locus: a tool for
pyramiding potyvirus resistance genes in pepper.
Genome,42(6): 1111-1116.
Caranta C, Lefebvre V, and Palloix A (1997). Polygenic
resistance of Pepper to potyviruses consists of a
combination of isolate-specific and broad-spectrum
quantitative trait loci, Mol. Plant-Microbe Interact, 10:
872-878.
Charron C, Nicolai M, Gallois JL, Robaglia C, Moury B
Palloix A and Caranta C (2008). Natural variation and
functional analyses provide evidence for co-evolution
between plant eIF4E and potyviral VPg. The Plant
Journal, 54(1): 56-68.
Çelik N, Özalp R ve Göçmen M (2012). Antalya İlinde
Örtü Altı Biber Yetiştiriciliğinde Patates Y Virüsü
(PVY) Patotiplerinin Belirlenmesi ve Bazı Biber
Çeşitlerinin PVY’ ye Karşı Reaksiyonları. Bitki
Koruma Bülteni, 52: 235-246.
Edwardson JR and Christie RG (1997). Viruses Infecting
Peppers and Other Solanaceous Crops. University of
Florida, Agricultural Experiment Station Institute of
Food and Agricultural Sciences, Volume 1: 336.
Ekbiç E, Abak K and Yılmaz MA (1997). New PVY
Pathotype on Pepper Along Mediterranean Coastal
Area of Turkey. Proc.10th Cong, Medit. Phytopath.
Union, 187-189, Montpellier France.
FAO(2016).http://faostat3.fao.org/wds/rest/exporter/strea
mexcel (Accessed to web: 19.11.2016).
Hamalainen JH, Watanabe KN, Valkonen JPT, Arihara A,
Plaisted RL, Pehu E, Miller L and Slack SA (1997).
Mapping and Marker-Assisted Selection for a Gene
for Extreme Resistance to Potato Virus Y, Theoretical
and Applied Genetics, 94: 192–197.
Hampton RO and Provvidenti R (1992). Specific
infectivity and host resistance have redicated potyviral
and pathotype nomenclature but relate less to
taxonomy. Arch. Virol. Suppl. 5: 183-187.
Heiser B (1973). Seed to civilization. The story of man's
food, Francisco.
Hwang J, Li J, Liu WY, An SJ, Cho H, Her NH, YeamI,
Kim D and Kang BC (2009). Double mutations in
eIF4E and eIFiso4E confer recessive resistance to
Chilli veinal mottle virus in pepper. Molecules and
cells, 27(3): 329-336.
Kaliciak A and Syller J (2009). New hosts of Potato virus
Y (PVY) among common wild plant in Europe.
Eurropen Journal of Plant Pathology, 124: 707–713.
Kang BC, Yeam I, Frantz JD, Murphy JF and Jahn MM
(2005). The pvr1 locus in Capsicum encodes a
translationin itiation factor eIF4E that interacts with
Tobacco etch virus VPg. Plant Journal, 42: 392–405.
Legnani R, Gebre-Selassie K, Nono-Womdim R,
Gognalons P, Moretti A, Laterrot H and Marchoux G,
(1995). Evaluation and Inheritance of the
Lycopersicon hirsutum Resistance Against Potato
Virus Y. Euphytica, 86: 219–226.
Lindhout P (2002). Theperspectives of polygenic
resistance in breeding for durable disease resistance.
Euphytica, 124: 217-226.
Maule AJ, Caranta C, and Boulton M I (2007). Sources of
natural resistance to Plant viruses: status and
prospects. Molecular Plant Pathology, 8 (2): 223-231.
Moury B, Morel C, Johansen E, Guilbaud L, Souche S,
Ayme V, Caranta C, Palloix A and Jacquemond M
(2004). Mutations in potato virus Y genome-linked
protein determine virulence towards recessive
resistance in Capsicum annuum and Lycopersicon
hirsutum. Molecular Plant- Microbe Interact, 17: 322-
329.
Palloix A, Abak K, Daubeze AM, Guldur M, Gebre and
Selassie K, (1994b). Survey of Pepper diseases
affecting the main production region of Turkey with
specialinterest in viruses and potyvirus pathotypes.
Capsicum Eggplant Newsletter, 13: 78-81.
Palloix A, Abak K, Gognalons P, Daubeze AM, Guldur M
Memouchı G and Gebre-Selaıssıe K (1994a). Virus
diseases infecting Pepper crops in Turkey. Congress
of the Mediterranean Phytopathological Union, 469-
472, Kuşadası Aydın Türkiye.
Robaglia C, and Caranta C (2006). Translation initiation
factors: a weak link in plant RNA virus infection.
Trends Plant Science, 11: 40-45.
Rubio M, Nicolai M, Caranta C and Palloix A (2009).
Allele mining in the pepper gene pool provided new
complementation effects between pvr2-eIF4E and
pvr6-eIF (iso) 4E alleles for resistance to pepper
veinal mottle virus. Journal of General
Virology, 90(11): 2808-2814.
Varveri C., 2000. Potato Y Potyvirus Detection by
Immunological and Molecular Techniques in Plants
and Aphids. Phytoparasitica, 28: 141-148
Yılmaz MA and Davis RF (1985). Identification of
Viruses Infecting Vegetable Crops along the
mediterranean sea Coast in Turkey. Journal Turkish
Phytopathology, 14: 1-8.
Yoon J Y, Green S K, Tschanz AT, Tsou SCA and Chang
L C (1989). Pepper Improment in the Tropics:
Problems and the Avrdc Approach. In: Proc Int Symp
Integrated Management Practices Tomato and Pepper
Production in the Tropics, 86-98, Tainan, Taiwan.

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