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Meme kanseri (MCF-7) ve Burkitt lenfoma (Raji) hücre dizileri üzerine in vitro ortamda hiperisin ile fotodinamik tedavi araştırma sonuçları

Results of in vitro photodynamic therapy with hypericin on breast cell (MCF-7) and Burkitt lymphoma (Raji) cell lines

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

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Abstract (2. Language): 
In our study we investigated the photodynamic effect of hypericin on breast cell (MCF-7) and Burkitt lymphoma (Raji) cell lines. The effect of hypericin administration on the proliferation of MCF-7 and Raji cell lines was investigated. Hypericin was administered between the concentrations of 1 and 50 µM for incubations of 24 and 48 hours as either a single regimen or after 24 joule/cm2 532 KTP laser (Orion) administration. Cytotoxity was evaluated with MTT test. To investigate the mechanism of antiproliferative effect, 1 nM staurosporine was added to the incubation media at 48th hour. The most significant effect was obtained on MCF-7 cell lines among the cell lines radiated. At 1 µM concentration of hypericin, 4% (p>0.05) and 41% (p<0.001) of cells died after 24 and 48 hours of incubation, respectively, whereas these rates were 48% (p<0.001) and 64% (p<0.001) at 50 µM concentration of hypericin, respectively. A significant increase in antiproliferative effect was observed in all cell lines after incubation of 24 and 48 hours at 1 and 50 µM concentrations (p<0.001 and p<0.001, respectively) in time-dependent evaluation. A significant increase in antiproliferative effect was observed in all concentrations when a protein kinase C inhibitor, staurosporine, was added after 48 hours of incubation with hypericin. Hypericin has a dose- and time-dependent antiproliferative effect on malignant cell lines such as MCF- 7 and Raji. The cytotoxic effect of hypericin increases with photodynamic activation depending on dose and incubation time. This effect is achieved with protein kinase C inhibition. As a result hypericin had antiproliferative effect on MCF-7 and Raji cell lines sampled in this study. Photodynamic therapy with hypericin may also be used for the treatment of intraocular tumors and further studies should be conducted.
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Abstract (Original Language): 
Çalışmamızda meme kanseri (MCF-7) ve Burkitt lenfoma (Raji) hücre dizileri üzerine hiperisinin fotodinamik etkinliği araştırılmıştır. Bir ve 50 μM konsantrasyon aralığında hiperisinin 24 ve 48 saatlik inkübasyonlarla tek başına ve 532 nm KTP lazer (Orion) ile uygulanan 24 jul/cm² dozunda ışınlama sonrası MCF-7 ve Raji hücre dizisi proliferasyonu üzerine olan etkileri değerlendirildi. Sitotoksisite değerlendirmesi MTT testi ile yapıldı. Antiproliferatif etkinin mekanizmasının öğrenilmesi açısından 48 saatlik kültür ortamına 1 nM konsantrasyonda staurosporin eklendi. Işınlama yapılan hücre dizileri üzerindeki etki MCF-7'de en belirgin olarak ortaya çıktı. MCF-7 dizisinde 1 μM konsantrasyonlarda 24. ve 48. saatlerde sırasıyla hücrelerin %4'ü (p>0.05) ve %41'i (p<0.001) ölürken, 50 μM konsantrasyonda bu oranlar %48 (p<0.001) ve %64 (p<0.001) idi. Zamana bağlı olarak değerlendirmede ise tüm hücre gruplarında 24. ve 48. saatlerde 1 ve 50 μM konsantrasyonlarda antiproliferatif etkide anlamlı artış gözlendi (p<0.001 ve p<0.001). Kırk sekiz saatlik hiperisin inkübasyonunda tüm dozlarda protein kinaz inhibitörü staurosporin eklendiğinde antiproliferatif etkide belirgin derecede artış gözlendi. Hiperisin, MCF-7 ve Raji gibi kötücül hücre dizilerine doza ve zamana bağımlı olarak antiproliferatif etki göstermektedir. Bu etkisi fotodinamik aktivasyonda doza ve zamana bağımlı olarak artmaktadır. Hiperisin bu etkisini protein kinaz C inhibisyonu üzerinden gerçekleştirmektedir. Sonuç olarak hiperisin, bu çalışmada örnek alınan MCF-7 ve Raji hücre dizinleri üzerine antiproliferatif etki göstermektedir. Göz içi tümörlerin tedavisinde de hiperisin ile fotodinamik tedaviden yararlanılabilir ve bu konu ile ilgili ileri çalışmalar planlanmalıdır.
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PDF icon arastirmax-meme-kanseri-mcf-7-burkitt-lenfoma-raji-hucre-dizileri-uzerine-vitro-ortamda-hiperisin-ile-fotodinamik-tedavi-arastirma-sonuclari.pdf
  • 3
147-152
  • Turkish

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