You are here

Home » Content

Göbek kordonu kanındaki kök hücre, lenfosit ve lenfosit alt gruplarının immünfenotipik olarak belirlenmesi

Immunophenotypic detection of umbilical cord blood stem cell, lymphocyte and lymphocyte subpopulations

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author Name
Abstract (2. Language): 
In this study it was aimed to differentiate stem cell, lymphocyte and lymphocyte subgroups in umbilical cord blood according to antigenic phenotypes and to determine the relative and absolute counts of these cells. Umbilical cord blood samples were collected from a total of 33 term newborns, 16 female (48.5%) and 17 male (51.5%), all of whom were born by spontaneous vaginal route without any complication during gestation or delivery. Lymphocyte and lymphocyte subgroups and CD34 stem cell ratios were detected by flow cytometry. Umbilical cord blood samples were compared with peripheral venous blood of 33 healthy adults and with bone marrow samples of 23 healthy adults regarding lymphocyte and lymphocyte subgroups, and CD34 stem cell, respectively. In umbilical cord blood, ratios of T cell, cytotoxic T cell and large granular lymphocytes were lower, and total B cell and T helper/suppressor ratios were higher in comparison to peripheral venous blood samples of adults (p<0.05). Numbers of all lymphocyte subgroups except for large granular lymphocytes were higher than those of the healthy adult samples because of the high number of leukocytes in umbilical cord blood (p<0.001). Ratio of CD34 stem cells in umbilical cord blood samples was higher than that of the samples of adult bone marrow (p<0.05). Significant absolute and relative differences were detected between the lymphocyte and lymphocyte subgroups of umbilical cord blood and peripheral adult venous blood, and umbilical cord stem cells and adult bone marrow
Bookmark/Search this post with
Abstract (Original Language): 
Bu çalışmada yenidoğan bebeklerin kordon kanlarındaki kök hücre, lenfosit ve lenfosit alt gruplarının antijenik fenotiplerine göre ayrımını yapmak ve bu hücrelerin göreceli ve mutlak sayılarını belirlemek amaçlanmıştır. Gebelik, travay ve do- ğum sırasında komplikasyon gözlenmeyen, normal vajinal yol ile dünyaya gelen, 16’sı kız (%48.5), 17’si erkek (%51.5) toplam 33 zamanında doğan yenidoğan bebekten kordon kanı örnekleri alındı. Lenfosit, lenfosit alt grupları ve CD34+ kök hücre oranları akım sitometrisi ile saptandı. Kordon kanı örnekleri, lenfosit ve lenfosit alt grupları açısından 33 sağlıklı erişkin çevre venöz kan örneğiyle; CD34+ kök hücre yönünden ise 23 sağlıklı erişkin kemik iliği örneğiyle karşılaştırıldı. Kordon kanında T hücre, sitotoksik T hücre ve büyük granüllü lenfosit oranları erişkin çevre kanına göre daha düşük; total B hücre ve T hücre helper/suppressor oranları ise daha yüksek saptandı (p<0.05). Kordon kanı beyaz küre sayılarının yüksekliğinden dolayı, büyük granüllü lenfositler hariç bütün lenfosit alt grup sayıları, erişkin örneklerine oranla daha yüksek saptandı (p<0.001). Kordon kanı örneklerinde CD34+ kök hücre oranı, erişkin kemik iliği örneklerine oranla daha yüksek değerde saptandı (p<0.05). Göbek kordonu kanı lenfosit ve lenfosit alt grupları, erişkin çevre venöz kanıyla, kordon kanı kök hücreleri ise erişkin kemik iliğiyle sayı ve orantısal olarak farklılıklar içermektedir.
FULL TEXT (PDF): 
PDF icon arastirmax-gobek-kordonu-kanindaki-kok-hucre-lenfosit-lenfosit-alt-gruplarinin-immunfenotipik-olarak-belirlenmesi.pdf
  • 4
261-266
  • Turkish

REFERENCES

References: 

aynaklar
1. Rocha V, Sanz G, Gluckman E. Umbilical cord blood
transplantation. Curr Opin Hematol 2004; 11:
375–385.
2. Cohen Y, Nagler A. Cord blood biology and
transplantation. Isr Med Assoc J 2004; 6: 39-46.
3. Gluckman E, Broxmeyer HA, Auerbach AD, et al.
Hematopoietic reconstitution in a patient with Fanconi’s
anemia by means of umbilical-cord blood from an HLAidentical sibling. N Engl J Med 1989; 321: 1174-1178.
4. Gluckman E, Rocha V. History of the clinical use of
umbilical cord blood hematopoietic cells. Cytotherapy
2005; 7: 219-227.
5. Laughlin MJ, Eapen M, Rubinstein P, et al. Outcomes
after transplantation of cord blood or bone marrow
from unrelated donors in adults with leukemia. N Engl
J Med 2004; 351: 2265–2275.
6. Locatelli F, Rocha V, Reed W, et al. Eurocord Transplant
Group. Related umbilical cord blood transplantation in
patients with thalassemia and sickle cell disease. Blood
2003; 101: 2137-2143.
7. Rocha V, Wagner JE Jr, Sobocinski KA, et al. Graftversus-host disease in children who have received a
cord-blood or bone marrow transplant from an HLAidentical sibling. Eurocord and International Bone
Marrow Transplant Registry Working Committee on
Alternative Donor and Stem Cell Sources. N Engl J Med
2000; 342: 1846-1854.
8. Ballard JL, Khoury JC, Wedig K, Wang L, EilersWalsman BL, Lipp R. New Ballard score expanded to
include extremely premature infant. J Pediatr 1991;
119: 417-423.
9. Erkeller-Yuksel FM, Deneys V, Yuksel B, et al. Age related
changes in human blood lymphocyte subpopulations. J
Pediatr 1992; 120: 216-222.
10. Heldrup J, Kalm O, Prellner K. Blood T and B lympocyte
subpopulations in healthy infants and children. Acta
Paediatr 1992; 81: 125-132.
11. Hulstaert F, Hannet I, Deneys V, et al. Age related
changes in human blood lymphocyte subpopulations.
II. Varying kinetics of percentage and absolute count
measurements. Clin Immunopathol 1994; 70: 152-158.
12. Bradstock KF, Kuxford C, Grimsley PG. Functional
and phenotypic assessment of neonatal human
lymphocytes expressing natural killer cell-associated
antigens. Immunol Cell Biol 1993; 71: 535-542.
13. Rabian-Herzog C, Lesage S, Gluckman E. Characterization
of lymphocyte subpopulations in cord blood. Bone
Marrow Transplant 1992; 9: 64-67.
14. Risdon G, Gaddy J, Stehman FB, Broxmeyer HE.
Proliferative and cytotoxic responses of human cord
blood T lymphocytes following allogenic stimulation.
Cell Immunol 1994; 154: 14-24.
15. Kinniburgh D, Russell NH. Comparative study of CD34-
positive cells and subpopulations in human umbilical
cord blood and bone marrow. Bone Marrow Transplant
1993; 12: 489-494.
16. Reisbach G, Bartke I, Kempkes B, et al. Characterization
of hematopoietic cell populations from human cord
blood expressing C-kit. Experiment Hematol 1993; 21:
74-79.
17. D’Arena G, Musto P, Cascavilla N, Di Giorgio G,
Zendoli F, Carotenuto M. Human umbilical cord
blood: immunophenotypic heterogeneity of CD34+
hematopoietic progenitor cells. Haematologica 1996;
81: 404-409.
18. Guo R, Ma B, Zou D. Analysis of immunophenotype
of hematopoietic stem/progenitor cells in human
umbilical cord blood with flow cytometry and its
significance. Zhonghua Xue Ye Xue Za Zhi 2001; 22:
426-428.
19. Sheridan WP, Begley CG, Juttner CA, et al. Effect
of peripheral-blood progenitor cells mobilised by
filgrastim (G-CSF) on platelet recovery after high-dose
chemotherapy. Lancet 1992; 339: 640-644.
20. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow
cytometric analysis of human bone marrow: I. Normal
erythroid development. Blood 1987; 69: 255-263.
21. Bender JG, Williams SF, Myers S, et al. Characterization
of chemotherapy mobilized peripheral blood progenitor
cells for use in autologous stem cell transplantation.
Bone Marrow Transplant 1992; 10: 281-285.
22. Mayani H, Dragowska W, Lansdorp PM. Characterization
of distinct subpopulations of CD34+ cord blood cells
in serum-free long-term cultures supplemented with
hematopoietic cytokines. Blood 1993; 82: 2664-2672.
23. Hiyama K, Hirayi Y, Kyoizumi S, et al. Activation of
telomerase in human lymphocytes and hematopoietic
progenitor cells. J Immunol 1995; 155: 3711-3715.266 • Aralık 2008 • Gülhane Tıp Derg Türkmen ve ark.
24. Vaziri H, Dragowska W, Allsopp RC, Thomas TE,
Harley CB, Lansdorp PM. Evidence for a mitotic clock
in human hematopoietic stem cells: loss of telomeric
DNA with age. Proc Natl Acad Sci USA 1994; 91:
9857-9860.
25. Hao Q-L, Shah AJ, Thiemann FT, Smogorzewska EM,
Crooks GM. A functional comparison of CD34+CD38-
cells in cord blood and bone marrow. Blood 1995; 86:
3745-3751.
26. Terstappen LW, Huang S, Safford M, Landsdorp PM,
Loken MR. Sequential generations of hematopoietic
colonies derived from single nonlineage-committed
CD34+CD38- progenitor cells. Blood 1991; 77:
1218-1227.
27. Van Epps DE, Bender J, Lee W, et al. Harvesting,
characterization and culture of CD34+ cells from
human bone marrow, peripheral blood and cord blood.
Blood Cells 1994; 20: 411-423.

Thank you for copying data from http://www.arastirmax.com