You are here

Enterococcus faecalis’in PMNL fonksiyonları üzerine etkisi: bir in vitro çalışma

The effects of Enterococcus faecalis on PMNL functions: an in vitro study

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (2. Language): 
The aim of this study was to evaluate the effects of E. faecalis on chemotactic and oxidative burst activity of polimorphonuclear leukocytes (PMNLs) using transwell system and flow cytometry. PMNL cultures of six healthy donors were incubated with or without E. faecalis culture supernatants in order to determine the effects of this microorganism on PMNL functions. In addition, the effects of soluble factors derived from active mononuclear cells on the PMNL functions against E. faecalis were tested in culture conditions. Chemotactic and oxidative burst activity were significantly higher in PMNL cultures with E. faecalis culture supernatants alone or combined with stimulated mononuclear cell culture supernatants than those of PMNL cultures without E. faecalis and stimulated mononuclear cell culture supernatants. Oxidative burst activity in PMNL cultures combined with E. faecalis and stimulated mononuclear cell culture supernatants were also higher than those of PMNL cultures with E. faecalis culture supernatants alone. In conclusion, increased PMNL functions by mononuclear cells may have possible contributions to tissue damage in the endodontic infections with E. faecalis. Additionally, soluble factors produced by active mononuclear cells may indirectly contribute to this process.
Abstract (Original Language): 
Bu çalışmanın amacı, kuyucuklu sistem ve akım sitometri kullanarak E. faecalis’in polimorfonükleer lökositlerin (PMNL) kemotaktik ve oksidatif patlama aktiviteleri üzerine olan etkilerini değerlendirmekti. Bu mikroorganizmanın PMNL fonksiyonları üzerine etkilerini tespit etmek için, altı sağlıklı vericiden hazırlanan PMNL kültürleri E. faecalis kültür süpernatanı ilave edilerek veya ilave edilmeden inkübe edildi. İlave olarak, aktive mononükleer hücrelerden kaynaklanan çözünür faktörlerin E. faecalis’e karşı PMNL fonksiyonları üzerine etkileri kültür koşullarında test edildi. Sadece E. faecalis kültür süpernatanı ile hazırlanan veya beraberinde uyarılmış MNH kültür süpernatanları içeren PMNL kültürlerinde kemotaktik ve oksidatif patlama aktiviteleri, E. faecalis ve uyarılmış mononükleer hücre kültür süpernatanları içermeyen PMNL kültürlerinden daha yüksekti. E. faecalis kültür süpernatanı ve beraberinde uyarılmış MNH kültür süpernatanı içeren PMNL kültürlerinde oksidatif patlama aktivitesi, sadece E. faecalis kültür süpernatanı içeren PMNL kültürlerinden de yüksekti. Sonuç olarak, E. faecalis nedenli endodontik enfeksiyonlarda artmış PMNL fonksiyonları doku hasarına katkıda bulunabilir. Ayrıca, aktive mononükleer hücrelerden üretilen çözünür faktörler dolaylı olarak bu sürece katkı sağlayabilir.
121-128

REFERENCES

References: 

References
1. Siqueira JF Jr, Rôças IN. Bacterial pathogenesis and
mediators in apical periodontitis. Braz Dent J 2007; 18:
267-280.
2. Skucaite N, Peciuliene V, Maciulskiene V. Microbial
infection and its control in cases of symptomatic apical
periodontitis: a review. Medicina (Kaunas) 2009; 45:
343-350.
3. Byström A, Sundqvist G. Bacteriologic evaluation
of the effect of 0.5 percent sodium hypochlorite in
endodontic therapy. Oral Surg Oral Med Oral Pathol
1983; 55: 307-312.
4. Safavi KE, Spangberg LSW, Langeland K. Root canal
dentinal tubule disinfection. J Endod 1990; 16: 207-210.
5. Buck RA, Eleazer PD, Staat RH, Scheetz JP. Effectiveness
of three endodontic irrigants at various tubular depths
in human dentin. J Endod 2001; 27: 206-208.
6. Ercan E, Dalli M, Yavuz I, Özekinci T. Investigation
of microorganisms in infected dental root canals.
Biotechnol Biotechnol Eq 2006; 20: 166-172.
7. Horiba N, Mackawa Y, Matsumoto T, Nakamura H. A
study of distribution of endotoxin in the dentinal wall
of infected root canals. J Endod 1990; 16: 331-334.
8. Berutti E, Marini R, Angeretti A. Penetration ability of
different irrigants into dentinal tubules. J Endod 1997;
23: 725-727.
9. Weiger R, de Lucena J, Decker HE, Löst C. Vitality status
of microorganisms in infected human root dentine. Int
Endod J 2002; 35: 166-171.
10. Love MR. Regional variation in root dentinal tubule
infection by Streptococcus gordonii. J Endod 1996; 22:
290-293.
11. Morrison D, Woodford N, Cookson B. Enterococci as
emerging pathogens of humans. Soc Appl Bacteriol
Symp Ser 1997; 26: 89S-99S.
12. Siren EK, Haapasalo MPP, Ranta K, Salmi P, Kerosuo
ENJ. Microbiological findings and clinical treatment
procedures in endodontic cases selected for
microbiological investigation. Int Endod J 1997; 30:
91-95.
13. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo
M. Isolation of yeasts and enteric bacteria in root-filled
teeth with chronic apical periodontitis. Int Endod J
2001; 34: 429-434.
14. Rocas IN, Siqueira JF Jr, Santos KR. Association
of Enterococcus faecalis with different forms of
periradicular diseases. J Endod 2004; 30: 315-320.
15. Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M.
Isolation of Enterococcus faecalis in previously rootfilled canals in a Lithuanian population. J Endod 2000;
26: 308-310.
16. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira
FB, Souza-Filho FJ. Microorganisms from canals of rootfilled teeth with periapical lesions. Int Endod J 2003;
36: 1-11.
17. Sundqvist G, Fidgor D, Persson S, Sjögren U.
Microbiologic analysis of teeth with failed endodontic
treatment and the outcome of conservative retreatment.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
1998; 85: 86-93.
18. Love RM. Enterococcus faecalis a mechanism for its role
in endodontic failure. Int Endod J 2001; 34: 399-405.
19. Shon W, Kim HS, Son HH, Lim S, Lee W. Effects of
sonicated Enterococcus faecalis extracts on interleukin-2
and interleukin-4 production by human T cells. J Endod
2004; 30: 701-703.
20. Nakamura K, Yamasaki M, Nishigaki N, et al. Effect
of methotrexate-induced neutropenia on pulpal
inflammation in rats. J Endod 2002; 28: 287-290.
21. Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating
peptide-1/interleukin-8, a novel cytokine that activates
neutrophils. J Clin Invest 1989; 84: 1045-1049.
22. Sannomiya P, Craig RA, Clewell DB, et al.
Characterization of a class of nonformylated
Enterococcus faecalis-derived neutrophil chemotactic
peptides: the sex pheromones. Proc Natl Acad Sci USA
1990; 87: 66-70.126 • June 2010 • Gulhane Med J Oktay et al.
23. Shon W, Lim S, Bae KS, Baek S, Lee W. The expression
of alpha4 integrins by human polymorphonuclear
neutrophils in response to sonicated extracts of
Enterococcus faecalis. J Endod 2005; 31: 369-372.
24. Ko HJ, Lim SS. Production of macrophage inflammatory
protein (MIP)-lalpha and MIP-lbeta by human
polymorphonuclear neutrophils stimulated with
Porphyromonas endodontalis lipopolysaccharide. J
Endod 2002; 28: 754-757.
25. Ember JA, Hugli TE. Characterization of the human
neutrophil response to sex pheromones from
Streptococcus faecalis. Am J Pathol 1989; 134: 797-805.
26. Kayaoğlu G. Virulance factors of Enterococcus faecalis
relationship to endodontic disease. Crit Rev Oral Biol
Med 2004; 15: 308-320.
27. Petrone WF, English DK, Wong K, McCord JM. Free
radicals and inflammation: superoxide-dependent
activation of a neutrophil chemotactic factor in plasma.
Proc Natl Acad Sci USA 1980; 77: 1159-1163.
28. Vanek NN, Simon SI, Jacques-Palaz K, Mariscalco
MM, Dunny GM, Rakita RM. Enterococcus faecalis
aggregation substance promotes opsonin independent
binding to human neutrophils via a complement
receptor type 3-mediated mechanism. FEMS Immunol
Med Microbiol 1999; 26: 49-60.
29. Rakita RM, Vanek NN, Jacques-Palaz K, et al.
Enterococcus faecalis bearing aggregation substance
is resistant to killing by human neutrophils despite
phagocytosis and neutrophil activation. Infect Immun
1999; 67: 6067-6075.
30. Pay S, Musabak U, Erdem H, Simşek I, Sengül A, Dinc
A. Chimerical anti-TNF-alpha, infliximab, inhibits
neutrophil chemotaxis and production of reactive
oxygen species by blocking the priming effect of
monoculer cells on neutrophils. Immunopharmacol
Immunotoxicol 2005; 27: 187-198.
31. Galligan C, Yoshimura T. Phenotypic and functional
changes of cytokine-activated neutrophils. Chem
Immunol Allergy 2003; 83: 24-44.
32. Ellis TN, Beamann BL. Interferon-gamma activation of
polymorphonuclear neutrophil function. Immunology
2004; 112: 2-12.
33. Berton G, Zeni L, Cassatella MA, Rossi F. Gamma
interferon is able to enhance the oxidative metabolism
of human neutrophils. Biochem Biophys Res Commun
1986; 138: 1276-1282.
34. Tennenberg SD, Fey DE, Lieser MJ. Oxidative priming
of neutrophils by interferon-gamma. J Leukocyte Biol
1993; 53: 301-308.

Thank you for copying data from http://www.arastirmax.com