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İSKELET ve KALP KASLARINDA LAKTİK ASİTİN TAŞINIMI: MONOKARBOKSİL TAŞIYICI PROTEİNLER BÖLÜM I

LACTIC ACID TRANSPORT ACROSS THE PLASMA MEMBRANE OF SKELETAL AND HEART MUSCLES: MONOCARBOXYLATE TRANSPORTERS PART I

Journal Name:

  • Hacettepe Üniversitesi Spor Bilimleri Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Lactic acid is both a major fuel for skeletal muscle (oxidative fibers) and a major meta¬bolic end product (glycolytic fibers). Therefore, lactate transport across the plasma memb¬rane is an important regulatory mechanism for lactate movement in and out of the skeletal muscle. Monocarboxylate transporters (MCTs) are proton-linked membrane carriers invol¬ved in the transport of monocarboxylates such as lactate, pyruvate, ketone bodies. They be¬long to a larger family of transporters composed of 14 members in mammals based on se¬quence homologies. It appears that some of these MCTs are expressed in a tissue-specific manner, whereas others are coexpressed in a number of tissues. There are two isoforms of MCTpresent in rat and human skeletal muscle and in the heart, MCT1 and MCT4. However, only skeletal muscle expresses both the MCT1 and MCT4 proteins, whereas rat heart exp¬resses the MCT1, but not the MCT4 protein. MCT1 expression is highly correlated with the oxidative fiber composition of the muscle. Lactate uptake from the circulation is also highly correlated with the MCT1 content of muscles. MCT4 is confined to fast-twitch (fast glycoly-tic and fast oxidative glycolytic) muscle fibers, in which MCT4 content is correlated with in¬dices of anaerobic metabolism. Therefore, these data suggest that MCT1 and MCT4 are pri¬marily responsible for lactate uptake from the circulation and lactate extrusion out of musc¬le, respectively.
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Abstract (Original Language): 
Laktik asit iskelet kasları için hem ana yakıt (oksidatif fibriller) hem de son üründür(gliko-litik fibriller). Bu nedenle laktatın plazma membranında taşınımı, onun kasın içine girişi ve çı¬kışı için önemli bir düzenleyici mekanizmadır. Monokarboksil taşıyıcı proteinler (MCT) laktik asit, pürivik asit ve keton cisimleri gibi monokarboksilli asitleri taşıyan proton bağlantılı taşı¬yıcı proteinleridir. MCT'ler memelilerde aminoasit içeriğine göre belirlenmiş 14 üyeden olu¬şan büyük bir taşıyıcı protein ailesidir. Bu proteinlerin bazıları belirli bir dokuya özgünken, diğer bazıları birçok dokuda mevcuttur. İnsan ve sıçanların iskelet ve kalp kaslarında MCT1 ve MCT4 olmak üzere iki izoform vardır. Bununla beraber iskelet kaslarında her iki izoform-da (MCT1 ve MCT4) mevcutken, sıçanların kalp kasında sadece MCT1 bulunur. MCT1 ile kasın oksidatif fibril kompozisyonu arasında yüksek ilişki vardır. Kasın MCT1 içeriği ile dola¬şımdan laktat alımı arasında da yüksek ilişki mevcuttur. MCT4 hızlı kasılan fibrillerle (hızlı gli-kolitik ve hızlı oksidatif glikolitik) sınırlıdır ve anaerobik metabolizma ile ilişkilidir. Böylece bu bilgiler MCT1'in öncelikle dolaşımdan kasa, MCT4'ün ise kasdan dolaşıma laktik asitin taşı-nımından sorumlu olduğunu gösterir
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