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ATLETLERDE KAS LİFİNİN ANTRENMAN GEÇMİŞİ VE CİNSİYET FARKLILIĞI İLE İLİŞKİLİ KANTİTATİF HİSTOKİMYASAL ÖZELLİKLERİ

QUANTITATIVE HISTOCHEMICAL PROPERTIES OF INDIVIDUAL MUSCLE FIBRE RELATED TO TRAINING BACKGROUND AND GENDER DIFFERENCES OF RUNNERS.

Journal Name:

  • Hacettepe Üniversitesi Spor Bilimleri Dergisi

Publication Year:

  • 1995

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Muscle biopsy samples were taken from the vastus lateralis muscle of 22 male and 8 female short- (SDR), middle- (MDR) and long-distance runners (LDR).Muscle crosssections were stained for the myofibrillar, ATPase, calciumstimulated myofibrillar ATPase (Ca2+-mATPase), succinate dehydrogenase (SDH) and ordinary PAS-stain. Comparative microphotometric measurements were performed for SDH and Ca2+-mATPase enzyme activities using a mic¬ rophotometer (Zeis MPM). The cross-sectional area and capillary density of fibers, which have been enzyme-histochemicaly measured, were calculated. The mean values of Ca2+-mATPase and SDH enzyme activities in single fibres were similar between histochemically similar fibre types of SDR, MDR and LDR subjects whereas the ratio between Ca2+mATPase and SDH in similar fibres showed remarkable differences among the groups. When the mean values of the ratio for single fibre types were treated with relative areas of fibre types as a marker of whole muscle (metabolic index, Ml), there was a net difference a¬ mong the three groups of athletes. In addition, it was found that female athletes had a higher SDH and lower Ca2+-mATPase: SDH ratio in type I, HA and type HA, IIB fibres, respectively, than their male counterparts. In conclusion, the results of this sutudy indicated that; 1) the ratio between Ca2+-mATPase and SDH in single fibres as a marker of ATP turnover rate is a valuable indicator to determine the metabolic properties of individual fibre related to training background of athletes, 2) the Ml value which involves the parameters of whole muscle sample can be better determinant than that of single fibre to express the metabolic properties of the muscle related to training background of athletes, 3) enlargement in fibre cross-sectional area has no significant change on the selected enzyme activities in the fibre and 4) male athletes have lower SDH and similar level Ca£+-mATPase enzyme activités whereas theyhave higher rate of Ca2+-mATPase: SDH ratio for histochemically similar fibre types compared to female counterparts, who had similar training backgrounds.
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Abstract (Original Language): 
22 erkek ve 8 bayan kısa- (KMA), orta- (OMA) ve uzun mesafe koşucusunun (UMA) vastus lateralis kaslarından biyopsi alındı. Kas örneklerinin kesitleri mi-yofibriller ATPase (mATPase), kalsiyumla uyarılan miyofibriller ATPase (Ca2+-mATPase), süksinil dehidrogenaz (SDH) ve PAS boyası ile boyandı. Kas örneklerinde SDH ve Ca2+-mATPase enzim aktivasyonlarının karşılaştırmalı mik-rofotometrik ölçümleri (Zeis MPM) yapıldı. Enzimatik ölçümleri yapılan kas liflerinin kesit alanları ve kapiller yoğunlukları hesaplandı. Histokimyasal olarak belirlenen kas liflerinin Ca24-mATPase ve SDH enzim aktivasyonlarının birbirine oranı (Ca2+-mATPase: SDH) aynı kas lif tipi için KMA, OMA ve UMA denekler arasında farklılıklar gösterirken bu enzimlerin tek başına aritmetik ortalama değerleri KMA, OMA ve UMA denekler arasında farklılıklar göstermemekteydi. Kas lif tiplerinin Ca2+-mATPase: SDH oranlarının bu tiplerinin relatif alan değerleri ile düzeltilmiş ve kas örneğinin tamamı için ifade edilen değerleri (Metabolik indeks Mİ) ise üç grup arasında daha net farklılıklar göstermekteydi. Ayrıca bayan denekler tip I ve HA kas lifleri için aynı grup erkek deneklerden daha yüksek SDH, tip HA ve IIB lifleri için ise daha düşük Ca2+mATPase: SDH oranına sahipti. Sonuç olarak bu çalışmanın bulguları; 1) kas lifi ATP döngüsünün bir göstergesi olarak ifade edilen Ca2+-mATPase: SDH oranının sporcunun antrenman geçmişi ile ilişkili metabolik özelliklerini irdelemede iyi bir parmetre olduğunu, 2) bütün kas liflerinin ölçüm sonuçlarını içinde barındıran Mİ değerinin antrenman geçmişi ile ilişkili iskelet kası metabolik özelliklerini belirlemede kas lifi için ifade edilen değerlerden daha değerli olduğunu, 3) kas lifinin kesitsel alanında olabilecek değişikliklerin bu çalışmada ölçülen enzimlerin aktivasyon düzeyleri üzerinde anlamlı değişikliklere yol açmadığı ve 5) benzer tipte antrenman geçmişine sahip o¬ lan erkek deneklerin aynı kas lifi tipi için bayan deneklerden daha düşük düzeyde SDH ve benzer düzeyde Ca2+mATPase enzim aktivasyonu değerlerine karşın daha yüksek Ca2+-mATPase: SDH oranı değerlerine sahip olduklarını gösterdi.
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