You are here

SUPRAMAKSİMAL EGZERSİZİN ERİTROSİT ANTİOKSİDAN ENZİMLER ÜZERİNE ETKİSİ

THE EFFECT OF SUPRAMAXIMAL EXERCISE ON THE ERYTHROC YTE A NTIOXIDA NT ENZYMES

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (2. Language): 
The effect of supramaximal physical activity on intracellular antioxidant enzyme activities is estimated in the present study. Two different supramaximal exercise protocols (Wingate and Modified Wingate) were pllanned and applied for this aim. Wingate protocol is an supramaximal activity that is applied with 75 g/kg load for 30 seconds and modified protocol is applied with same load for 6 seconds followed 30 seconds of resting phase for 10 times. Ten healthy sedentary male volunteers (aged between 18-25 years) and 10 male bicyclists (aged among 16-28 years) were participated in the study. Blocd samples were drawn before and after Wingate and modified Wingate protocol and erythrocyte superoxide dismutase (SOD) and catalese activities and plasma and erythrocyte Cu, Zn levels were measured. Statistical analyses were performed with student t test. It was thought that supramaximal exercise caused production of excessive amount of free radicals and consequently oxidant stress and trained individual possesses more powerful capacity of antioxidant defense in comparison with sedentary individuals.
Abstract (Original Language): 
ÖZET Sunulan çalışmada supramaksimal kassal aktivitenin intrasellüler antioksi-dan enzim aktiviteleri üzerindeki etkisi araştırılmıştır. Bu amaçla 2 farklı supramaksimal egzersiz protokolü (Wingate ve Modifiye Wingate) programlanmış ve uygulanmıştır Wingate protokolü bireyin vücut ağırlığı başına 75 gramlık yükle yapılan 30 saniyelik supramaksimal bir aktivitedir. Modifiye protokolü ise yine aynı yükle herbiri 6 saniyelik egzersiz 30 saniyelik dinlenim olmak üzere toplam 10 supramaksimal egzersiz periyodundan oluşmaktadır Deneklerden egzersizden önce ve hemen sonra alınan kan örneklerinde eritrosit süperoksit dismutaz (SOD) ve katalaz aktiviteleri ile plazma ve eritrosit Cu, Zn düzeyleri saptanmıştır Tüm bulgular spektrofotometrik olarak ölçülürken, istatistiksel olarak t testi ile değerlendirildi. Çalışma 18-25 yaşları arasındaki 10 sağlıklı sedanter erkek ve 16-28 yaşları arasındaki 10 erkek bisiklet sporcusu üzerinde yapıldı. Deneklerin Wingate ve Modifiye Wingate protokolünden önce ve sonra SOD ve katalaz aktiviteleri, eritrosit ve plazma element seviyeleri ölçüldü ve karşılaştırıldı. Supramaksimal egzersizin çok fazla miktarda serbest radikal oluşumu ve dolayısıyla oksidan strese yol açtığı, antrenmanlı bireylerin sedantere oranla daha güçlü bir antioksidan savunma kapasitesine sahip oldukları kanısına varıldı.

REFERENCES

References: 

Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-6.
Alessio H. M.& Goldfarb A.H. (1988). Lipid peroxidation and scavenger enzymes during exercise: Adaptive response to training. J. Appl. Physiol. 64:1333-6.
Cao, G.& Chen, J. (1991). Effects of dietary zinc on free radical generation, lipid peroxidation, and superoxide dismutase in trained mice. Arch. Biochem. Blophys. 291(1) 147-153.
Clark, I. A., Cowden, W. B. & Hunt, N. H. (1985). Free radical-induced Pathology. Med. Res. Rev, 5:297-332.
Cotran, R.S., Kumar, V.& Robbins, S. L.(1989). Cellular Injury and Adaptations. Robbins Pathologic Basis of Disease, 4. Edition, Philadelphia: W. B. Saunders Company, S.1-38.
Davies, K. J. A., Quintanilha, A. T., Brooks, G. A.& Packer, L. (1982). Free radicals and tissue damage by exercise. Biochem. Blophy. Res. Com. 107:1198-1205
Gohil, K., Viguie, C, Stanley, W.C., Brooks, G.A.& Packer, L. (1988). Blood glutathione oxidation during human exercise. J.Appl. Physiol. 64:115-9.
Higushl, M., Cartier, L. J., Chen, M.& Holloszy, J. O. (1985). Superoxide dismutase and catalase in skeletal muscle: Adaptive response to exercise. J. Gerontol. 40:281-86.
Jenkins, R.R. (1988). Free radical chemistry. Relationship to exercise. Sports Med. 5(3):156-70.
Ji, L. L. (1995). Exercise and oxidative stress: Role of cellular antioxidant systems. Exercise and Sport Science Reviews, edited by J.O. Holloszy. Baltimore, MD: Williams and Wilklns, vol 23 p135-66
Ji, L. L., Dillon, D.& Wu, E. (1990). Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am. J. Physiol. 258.R918-R923.
Ji, L. L., Stratman, F. W.& Hardi, H. A. (1988). Antioxidant enzyme systems in rat liver and skeletal muscle. Influences of selenium deficiency, chronic training, and acute exercise. Arch. Biochem. Bio-phys. 263(1):150-60.
22
Antioksidan Enzimler
Kanaley,
J
. A.& Ji, L. L. (1 991). Antioxidant enzyme activity during prolonged exercise in amenorrhea and eumenorrheic athletes. Metabolism. Vol 40, 1:88-92.
Kanter, M. M., Hamlin, R.L, Unverfelt, D.V., Davies, H.W.& Merola, A.J. (1985). Effect of exercise training on antioxidant enzymes and carditoxicity of doxorubicin. J. Appl. Physiol 59(4): 1298-1303.
Kihlstrom, M., Ojala, J.& Salminen, S. (1989). Decreased level of cardiac antioxidants in endurance-trained rats. Acta Physiol. Scand. 135:549-54.
Kono, I.& Fridovich, I. (1982) Superoxide radical inhibits catalase. J Biol Chem 257:5751-5.
Laughlin, M.H., Simpson, T, Sexton, W.L, Brown, O.R., Smith, J.K.& Kortlus, R.J. (1990). Skeletal muscle oxidative capacity, antioxidant enzymes, and exercise training. J. Appl. Physiol. 68:2337-43.
Lawler, J., Powers, S.K., Visser, T, Van Dijk, H., Kortius, M.J.& Ji, L.L. (1993). Acute exercise and skeletal muscle antioxidant and metabolic enzymes: effect of fiber type and age. Am. J. Physiol. 265:R1344-R1350.
Leewenbourg, C, Fiebig, R., Chandwaney, R.& Ji, L.L. (1994). Aging and exercise training in skeletal muscle: response of glutathione and antioxidant enzyme systems. Am. J. Physiol. 267: R439-R445.
Mena, P., Maynar, M., Gutierrez, J.M., Maynar, J., Timon, J.& Campillo, J.E. (1991). Erythrocyte free radical scavenger enzymes in bicycle professional racers. Adaptation to training . Int. J. Sport Med. 12(6)563-6.
Oh-ishi, S., Kizaki, T., Nagasawa, J. ve arkadaşları (1997). Effects of endurance training on superoxide dismutase activity, content and mRNA expression in rat muscle. Clin. Exp. Pharmacol. Physiol. 24:326-32.
Ohno, H., Yahata, T., Sato, Y, Yamamura, K.& Tanuguchi, N. (1988). Physical training and fasting erythrocyte activities of free radical scavenging enzyme systems in sedentary men. J. Appl. Physiol. 57:173-6, 1988
Ohno, H., Sato.Y, Yamashita, K. ve arkadaşları. (1986). The effect of brief physical exercise on free radical scavenging enzyme systems in human red blood cells. Can. J. Physiol. Pharmaco. 165:1263-65,
Ostenblad, N., Madsen, K.& Djurhuus, M.S. (1997). Antioxidant status and lipid peroxidation after short term maximal exercise in trained and untrained humans. Am. J. Physiol. 272: R 1258-R 1263.
Packer, L. (1989). Oxidants and antioxidants and the biological effects of physical exercise. Biological Effects of Physical Activity, Edited by Williams R.S., Wallace A.G., U.S.A: Human Kinetics Publishers, 5.85-90.
Powers, S.K., Criswell, D., Lawler, J. ve arkadaşları (1993). Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. Am. J. Physiol. 265: H2094-H2098.
Powers, S.K., Criswell, D., Lawler, J. ve arkadaşları (1994). Influence of exercise fiber type on antioxidant enzyme activity in skeletal muscle. Am. J. Physiol. 266:R375-R380.
Quintanilha, AT. (1984). The effect of physical exercise and/or vitamin E on tissue oxidative metabolism. Biochem. Soc. Trans. 12:403-404.
23
Zergefoglu, Yavuzer
Salminen, A.& Vihko, V. (1983). Endurance training reduces the susceptibility of mouse skeletal muscle to lipid peroxidation in vitro. Acta Physiol. Scand. 117:109-13.
Salminen, A., Kainulainen, H.& Vihko, V. (1984). Endurance training and antioxidants of Lung. Ex-perelntla, 40:822-23.
Slater, T.F. (1984). Free radical mechanisms in tissue injury. Blochem. J. 222:1-15.
Shepard, R.J. (1989). Effects of exercise on biological features of aging. Biological Effects of Physical Activity, Edited by Williams R. S., Wallace A. G., U.S.A.: Human Kinetics Publishers, 5.55-70.
Tiidus, P.M., Pushkarenko, J.& Houston, M. E. (1996). Lack of antioxidant adaptation to short term aerobic training in human muscle. Am. J. Physiol. 271 :R832-R836.
Weiss, S. J. (1986). Oxygen, ischemia and inflammation. Acta Physiol. Scand. Suppl. 548:9-37.
William, R, Graettinger, M.D.& Albuquerque, N.M. (1984). The Cardiovascular response to physical exertion and exercise training: An Echocardiographlc review. American Heart Journal, Vol. 108 4:1014¬18.
Winterbourn, C.C., Hawkins, R. E., Brian, M.& Carrel, R.W. (1975). The estimation of red cell superoxide dismutase activity. J. Lab. Clin. Med. 85:337-41

Thank you for copying data from http://www.arastirmax.com