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SUPRAMAKSİMAL EGZERSİZİN ERİTROSİT ANTİOKSİDAN ENZİMLER ÜZERİNE ETKİSİ

THE EFFECT OF SUPRAMAXIMAL EXERCISE ON THE ERYTHROC YTE A NTIOXIDA NT ENZYMES

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Abstract (2. Language): 
The effect of supramaximal physical activity on intracellular antioxidant enzyme activities is estimated in the present study. Two different supramaximal exercise protocols (Wingate and Modified Wingate) were pllanned and applied for this aim. Wingate protocol is an supramaximal activity that is applied with 75 g/kg load for 30 seconds and modified protocol is applied with same load for 6 seconds followed 30 seconds of resting phase for 10 times. Ten healthy sedentary male volunteers (aged between 18-25 years) and 10 male bicyclists (aged among 16-28 years) were participated in the study. Blocd samples were drawn before and after Wingate and modified Wingate protocol and erythrocyte superoxide dismutase (SOD) and catalese activities and plasma and erythrocyte Cu, Zn levels were measured. Statistical analyses were performed with student t test. It was thought that supramaximal exercise caused production of excessive amount of free radicals and consequently oxidant stress and trained individual possesses more powerful capacity of antioxidant defense in comparison with sedentary individuals.
Abstract (Original Language): 
ÖZET Sunulan çalışmada supramaksimal kassal aktivitenin intrasellüler antioksi-dan enzim aktiviteleri üzerindeki etkisi araştırılmıştır. Bu amaçla 2 farklı supramaksimal egzersiz protokolü (Wingate ve Modifiye Wingate) programlanmış ve uygulanmıştır Wingate protokolü bireyin vücut ağırlığı başına 75 gramlık yükle yapılan 30 saniyelik supramaksimal bir aktivitedir. Modifiye protokolü ise yine aynı yükle herbiri 6 saniyelik egzersiz 30 saniyelik dinlenim olmak üzere toplam 10 supramaksimal egzersiz periyodundan oluşmaktadır Deneklerden egzersizden önce ve hemen sonra alınan kan örneklerinde eritrosit süperoksit dismutaz (SOD) ve katalaz aktiviteleri ile plazma ve eritrosit Cu, Zn düzeyleri saptanmıştır Tüm bulgular spektrofotometrik olarak ölçülürken, istatistiksel olarak t testi ile değerlendirildi. Çalışma 18-25 yaşları arasındaki 10 sağlıklı sedanter erkek ve 16-28 yaşları arasındaki 10 erkek bisiklet sporcusu üzerinde yapıldı. Deneklerin Wingate ve Modifiye Wingate protokolünden önce ve sonra SOD ve katalaz aktiviteleri, eritrosit ve plazma element seviyeleri ölçüldü ve karşılaştırıldı. Supramaksimal egzersizin çok fazla miktarda serbest radikal oluşumu ve dolayısıyla oksidan strese yol açtığı, antrenmanlı bireylerin sedantere oranla daha güçlü bir antioksidan savunma kapasitesine sahip oldukları kanısına varıldı.
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