You are here

Isolation And Purification Of Plant Growth Promoting Rhizobacteria (Pgpr) From The Rhizosphere Of Acorus Calamus Grown Soil

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (Original Language): 
Among the 10 bacterial isolates were obtained from Acorus calamus rhizospheric soil of Melaiyar and Nagapattinam districts in Tamil Nadu. All the isolates were identified as Azospirillum spp., Bacillus spp., Pseudomonas spp., and Azotobacter spp. These bacterial strains were tested on morphological, biochemical and screened for their direct growth promoting activities (IAA production, production of Ammonia and Phosphate solubilization) and indirect growth promoting activities (HCN production, Siderophore production). The results obtained showed that among the 10 isolates of Melaiyar and Nagapattinam districts (M-M15) of ranged from (4.10 - 5.66 and 4.40 - 7.80) of Azospirillum spp., (3.00 – 6.00 and 3.20 – 6,.30 ) of Bacillus spp., (5.00 – 8.40 and 5.10 - 8.20) of Pseudomonas spp., and (3.00 - 6.00 and 3.30 - 6.30) of Azotobacter spp. The IAA production of Pseudomonas spp. (94%), Azospirillum spp (80%), Azotobacter spp. (65%) and Bacillus spp. (40%). Ammonium production of the isolates, Bacillus spp. (96%), Pseudomonas spp. (92%), Azospirillum spp. (65%) and Azotobacter spp. (55%). The siderophore and HCN production produced by all the isolates make it suitable for further investigation of pot and field trials by Acorus calamus cultivation
1-13

REFERENCES

References: 

1. Allen, O.N. 1953. Experiments in Soil bacteriology. Burgess Publ. Co., Minneapology Minnesota, U.S.A.,
pp. 69-70.
2. Barazani, O., and J. Friedman, 2000. Effect of exogenously applied L-tryptophan on allelochemical
activity of plant growth promoting rhizobacteria (PGPR).J.Chem.Ecol., 26:343-349.
3. Brick,J.M., bostock,R.M and ssilverstones, S.E 1991.Rapid in situ assay for indole acetic acid production
by bacteria immobilized on nitrocellulose membrane Appl.Environ.Microbial.,57:535-538.
4. Cappuccino, J.C. and Sherman, N.1992. In: microbiology;A laboratory manual therd ed. Benjamin/
cummings pub.Co., New York,pp.125-179.
5. Crozier, A and P. Arrude, 1988. Analysis of Indole-3-acetic acid and related insoles in cultures medium
from Azospirillum lipoferum and Azospirillum brasilense. Appl. Environ. Microbiol., 54:2833-2837.
6. Dobereiner. J and Day. M, 1957. Associative symbiosis in tropical grasses, characterization of
microorganisms and nitrogen fixing sites, in : W.E. Newton, C.J. Nyman (Eds), Proceeding of the 1st
international symposium on nitrogen fixation, vol.,2 Washington state University Press, Pullmann, pp.518-
588.
7. Gaur, A.C, 1990. Physiological functions of phosphate solubilizing microorganisms. In: gaur, A.C. (Ed.),
Phosphate solubilizing microorganisms as Biofertilizers. Omega Scientific publishers, New Delhi, pp.16-
72.
8. Geetha, S. 2003. Studies on the mycotrophy of certain medicinal herbs and its antimicrobial property.
M.Sc. (Ag). Thesis, Annamalai University, Annamalai Nagar.
9. Glick, B.R. 1995. The enhancement of plant growth by free living bacteria. Can J. Microbiol., 41:109-114.
10. Gonalez-Lopez, J., V. Salmeron, M.V. Martinez-Toledo, F. Ballesteros and A. Ramos-Cormenzana, 1986.
Production of auxins, gibberellins and cytokinins by Azotobacter vinelandii ATTC 12837 is chemically
defined media and dialyzed soil media. J. sci. Ind. Res., 57:720-725.
11. Gopal, H. 2004. Development of microbial consortium for improvement of growth, yield and alkaloid
content of Aswagandha. Ph.D. Thesis, Tamil Nadu Agricultural University, Coimbatore.
12. Govinda Rao, Y.S., C.K. Suresh, N.S. Suresyh, and R.R. Mallikarajunaiah, 1987. Current Research.,
16:144-145.
13. Karthikeyan, B., C. Abdul Jaleel, G.M.A. Lakshmanan and M. Deiveeka sundaram, 2008. Studies on
rhizosphere microbial diversity of some commercially important medicinal plants. Colloids and surfaces
B: Biointerfaces., 62:143-145.
14. Kindscher and Kelly. Medicinal wild plants of the prairie university press of Kansas, Lawrence kansas
1992.
15. King, J.E 1982.the colorimetric determination of phosphorus. Biochem.J.,26:292 .
16. King’s, E.O., M.K. Ward and D.E. Rency, 1954. Two sample media for the demonstration of pyocyanin
and fluorescin. J. Lab. Clin. Med., 44:301-307.
17. Kloepper, J.W., D.J. Hume, F.M. Scher and C. Singleton, 1988. Plant growth promoting rhizobacteria on
canola (rape seed). Plant. Dis., 72:42-46.
18. Loper, J.E and scroth, M.N.1986 Influence on bacterial sources on indole-3-acetic acid on elongation of
sugarbeet phytology,76:386
19. Lork, H.1948 production of hydrocyanic acid by bacteria. Physiol. Plant., 1:142-146.
20. Nieto, K.F., and W.T. Frankenberger, 1989. Biosynthesis of cytokinins produced by Azotobacter
chroococcum. Soil Bio. Biochem., 21 : 967-972.
21. Okon, Y., and C.A. Labandera-Gonalez, 1994. Agronomic applications of Azospirillum. In: Ryder, M.H.,
Stephens, P.M., Bowen, G.D. (Eds.), Improving plant productivity with Rhizosphere bacteria. Common
wealth scientific and industrial research organization, Adelaide, Australia, pp. 274-278.
22. Parekh J. Chanda V. In vitro Antimicrobial activity and photochemical analysis of home Indian medicinal
plants, Turkish journal of biology. 2007; 31:53-58.
23. Sabitha RA, Sutyakala M, Sandya DV, Suryanarayanana MU. Evaluation of Antibacterial activity from
rhizome extract of Acorus calamus linn. Journal of sciences and Indian research, 2003; 62(6): 529-650.
24. Schwyn,B and neilands, J.B. 1987. Universal chemical assay FOR detection and determination of
siderophores. Anal. Biochem., 160:47-56.
25. Venskutonies, Petras R, Dagilyte A. Compontion of essential oil of sweet flag (Acorus calamus, L.) leaves
at different growing phases. J. Essential oil research. 2003; 46-47.
26. Xie, H., J.J. Pasternak and B.R. Glick, 1996. Isolation and characterization of fmutants of plant growth
promoting rhizobacterium Pseudomonas putida GR 12-2 that over produce indole acetic acid. Curr.
Microbial., 32:67.71.

Thank you for copying data from http://www.arastirmax.com