You are here

Home » Content

Zidovudine karşı HIV rezistansının moleküler temeli ve rezistansın polinteraz zincir reaksiyonu ile saptanması

Molecular basis of HIV resistance to zidovudine and its detection by polymerase chain reaction (PCR)

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 1994

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Zidovudine (AZT) therapy decreases- both mortality and the incidence of opportunistic injections in the patients infected with human immunodeficiency virus (I111'). The resistance to AZT. however, is an im/wrtant problem. This resistance is associated with the point mutations at codon 41 (Met to Leu). 6~ (Asp to Asn). ~() (Lys to Arg). 215 (Thr to Phe or Tyr) and 219 (Lys to (ilu) in the reverse transcriptase coding region of ' pot gene. PCR amplification technique is used most commonly to determine these mutations. /.Journal of Turgut Özal Medical Center I(4):321-323.1994/
Bookmark/Search this post with
Abstract (Original Language): 
Zidovudin ile tedavi, human immunodeficiency virus (HIV') ile infekte hastalarda gözlenen fırsatçı infeksiyonların insidansını ve mor tali teyi azaltmaktadır. Ancak zidovudine karsı direnç gelişimi önemli bir problem oluşturmaktadır. Bu rezistans; H1V pol gen bölgesinde revers transkriptaz enzimini kodlayan alandaki 41 (Met—*Leu) inci, 67 (Asp—>Asn)'inci, "0 (I,ys—>Arg) inci. 215 (Thr—>Phe veya Thy)'inci ve 219 (Lys—>(ilu) 'uncu kolonlarda meydana gelen nokta mutasyonlarla ilgilidir. Bu mutasyonların saptanmasında PCR amplifikasyon tekniği çok yaygın olarak kullanılmaktadır. [Turgut Özal Tıp Merkezi Dergisi l(4):32l-323.1994]
FULL TEXT (PDF): 
PDF icon arastirmax-zidovudine-karsi-hiv-rezistansinin-molekuler-temeli-rezistansin-polinteraz-zincir-reaksiyonu-ile-saptanmasi.pdf
  • 4
321-323
  • Turkish

REFERENCES

References: 

1. Wainberg MA, Gu Z, Gao Q et al. Clinical correlates and molecular basis of HIV drug resistance. AIDS 1993;6(Suppl 1). S36-S46.
2. Kozal MJ, Merigan TC. HIV resistance to dideoxynucleoside inhibitors. Infectious Diseases in Clinical Practice 1993; 2:247-53.
3. St.Clair MH, Hartigon PM, Andrews JC et al. Zidovudine resistance, syncytium-inducing phenotype, and HIV disease progression in a case-control study. J Acquir Immune Defic Syndr 1993; 6:891-7.
4 Larder BA, Darby G, Richman DD. HIV with reduced sensitivity to zidovudine (AZT) isolated during prolonged therapy. Science 1989;243:1731-4.
5. Boucher CAB, Tersmette M, Lange JMA et al. Zidovudine sensitivity of human immunodeficiency virüse from high-risk symptome-free individuals during therapy. Lancet 1990;336:585-90.
6. Larder BA, Coates KE, Kemp SD. Zidovudine-resistant human immunodeficiency virus selected by passage in cell culture. J Virol 1991; 65.5232-6.
7 Kellam P, Boucher CAB, Larder BA. Fifth mutation in human immunodeficiency virus type
1 reverse transcriptase contributes to the development of high-level resistance to zidovudine. Proc Natl Acad Sci USA 1992; 89: 1934-8.
8. Larder AB, Boucher CAB. PCR detection of human immunodeficiency virus drug resistance mutants. In Diagnostic Molecular Microbiology. Principles and Applications. Persing DH, Smith TF, Tenover FC, White TJ (eds). Washington DC, American Society For Microbiology 1993:527-33.
9. Richman DD, Guatelli JC, Grimes J et al. Detection of mutations associated with zidovudine resistance in human immunodeficiency virus by use of the polymerase chain reaction. J Infect Dis 1991;164:1075-81.
10. Mcleod GX, Hammer SM. Zidovudine. Five years later. Ann Intern Med 1992; 117:487-501.
11. Gingeras TR, Prodanovich P, Latimer T et al. Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus. J Infect Dis 1991; 164:1066-74.
12 Rooke R, Tremblay M, Soudeyns H et al. Isolation of drug-resistant variants of HIV-1 from patients on long-term zidovudine therapy. AIDS 1989; 3:411-5.
13. Larder AB, Kemp SD. Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT). Science 1989; 246:1155-8.
14. St.Clair MH, Martin JL. Tudor-Williams G et al. Resistance to ddl and sensitivity to AZT induced by a mutation in HIV-1 reverse transcriptase. Science 1991;253:1557-9.
15. Sambrook J, Fritsch EF. Maniatics T. Molecular Cloning. A laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, USA 1989.

Thank you for copying data from http://www.arastirmax.com