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Psöriatik Deride Histopatolojik Bulgular ve Laminin ve Fibronektin Dağılımı

Histopathological Finding s and the Distribution of Laminin and Fibronectin in Psoriatic Skin

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Some previous studies have shown important modifications of the basement membrane of psoriatic skin, which could play a key role in alterations of keratinocyte proliferation and differentiation. Changes in distribution of the laminin a1 chain, together with fibronectin, might influence keratinocyte growth, and thus could be responsible for the initiation of psoriasis. In the view of these considerations, we have aimed to determine the expression pattern of laminin and of fibronectin, besides the structural alterations of the epidermis, dermis, and dermoepidermal junction in involved psoriatic skin. Material and methods: Fourteen male psoriasis vulgaris patients and 6 healthy volunteers were studied retrospectively. All psoriatic patients had chronic and stable psoriatic plaque, which had not been treated. Sections were stained with haematoxylin and eosin (H-E), and periodic acid Schiff (PAS) reagent, and were stained immunohistochemically using laminin and fibronectin kits. Results: Histological examination of psoriatic skin showed elongated rete ridges, parakeratosis, epidermal intercellular oedema, exositosis of mononuclear cells into the epidermis, and mononuclear cell inflammation in the dermis. PAS staining revealed the large interruptions of the basement membrane. In psoriatic skin samples, the staining of laminin showed discontinuous, weak, and an uneven disruption in the basement membrane. Staining for fibronectin was pronounced below the basement membrane and showed a reticular or fibrillar pattern in the dermis. Conclusion: The results of our study support the hypothesis that basement membrane alterations together with the alterations in the distribution pattern of laminin and fibronectin may play an important role in the pathogenesis of psoriasis.
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Abstract (Original Language): 
Amaç: Yapılan bazı çalışmalarda psöriatik deride keratinosit proliferasyonu ve farklılaşmasında anahtar rol oynayabilecek bazı önemli bazal membran değişiklikleri olduğu gösterilmiştir. Laminin a1 zinciri ve fibronektin dağılımındaki değişiklikler keratinosit büyümesini etkileyerek psöriasisin başlamasından sorumlu olabilir. Bu görüş doğrultusunda çalışmamızda psöriatik deride dermis, epidermis ve dermo-epidermal bileşkedeki yapısal değişikliklerin yanısıra laminin ve fibronektin dağılım örneğini de incelemeyi amaçladık. Gereç ve Yöntem: Ondört erkek psöriasis vulgaris hastası ve 6 sağlıklı gönüllü retrospektif olarak değerlendirildi. Psöriasisli hastalar kronik ve stabil plakları olan, tedavi almamış hastalardı. Kesitler haematoxylin ve eosin (H-E) ve periodic acid Schiff (PAS) yöntemleri ve laminin ve fibronektin kitleri kullanılarak immunohistokimyasal olarak boyandı. Bulgular: Psöriatik derinin histolojik incelenmesinde rete çıkıntılarında uzama, parakeratoz, epidermal intersellüler ödem, mononükleer hücrelerin epidermise geçişi ve dermiste mononukleer hücre infiltrasyonu saptandı. PAS yöntemiyle bazal membranda geniş kesintiler gözlendi. Psöriatik deride bazal membranda laminin zayıf, kesintili ve düzensiz bir dağılım örneği gösterdi. Fibronektin bazal membran altında belirgindi, dermisde fibriler veya retiküler bir boyanma örneği gösterdi. Sonuç: Çalışmamızın sonuçları psöriasisin patogenezinde bazal membran değişiklikleri ile birlikte laminin ve fibronektin dağılımındaki değişikliklerin önemli rol oynadığı hipotezini desteklemektedir.
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  • 4
217-222
  • Turkish

REFERENCES

References: 

1. Mckay IA, Leigh IM. Altered keratinocyte growth and differentiation in psoriasis. Clin Dermatol 1995;13:105-114.
2. Kormeili T, Lowe NJ, Yamauchi PS. Psoriasis; immunopathogenesis and evolving immunomodulators and systemic therapies; U.S. experiences. Br J Dermatol
2004;151(1):3-15).
3. Guilhou JJ. Immunopathogenesis of psoriasis : news in an old concept. Dermatology
1998;197:310-312.
4. Vaccaro M, Maggauda L, Cutroneo F, et al. Changes in the distribution of laminin a1 chain in psoriatic skin: immunohistochemical study using confocal laser scanning
microscopy. Br J Dermatol 2002;146(3):392-398.
5. Bernard BA, Robinson SM, Vandaele S, Mansbridge JN, Darmon M. Abnormal maturation pathway of keratinocytes in psoriatic skin. Br J Dermatol 1985;112(6):647-
653.
6. Bata-Csorgo Z, Cooper KD, Ting KM, Voorhees JJ, Hammerberg C. Fibronectin and alpha 5 integrin regulate keratinocyte cell cycling. A mechanism for increased fibronectin potentiation of T cell lymphokine-driven keratinocyte hyperproliferation
in psoriasis. J Clin Invest 1998;101(7):1509-1518.
7. Beta-Csorgo Z, Hammerberg C, Voorhees JJ, Cooper KD. Kinetics and regulation of human keratinocyte stem cell growth in short-term primary ex vivo culture. Cooperative growth factors from psoriatic lesional T lymphocytes stimulate proliferation among psoriatic uninvolved, but not normal, stem keratinocyes. J Clin
Invest 1995;95:317-327.
8. Aumailley M, Smyth N. The role of laminins in basement membrane function. J Anat
1998 193 (Pt 1):1-21.
9. Vaccaro M, Pergolizzo S, Mondello MR, et al. The dermoepidermal junction in psoriatic skin as revealed by scanning electron microscopy. Arch Dermatol Res
1999;291;396-399.
221
Eşrefoğlu et al
10. Mondello MR, Magaudda L, Pergolizza S, et al. Behaviour of laminin 1 and type IV collagen in uninvolved psoriatic skin. Immunohistochemical study using confocal laser scanning microscopy. Arch Dermatol Res 1996;288:527-531.
11. Fleming KA The skin. In: McGee JO'D, Isaacson PG, Wright NA, editors. Oxford Textbook of Pathology. Oxford : Oxford university press. 1992. p. 2146-2147.
12. Kawashima K, Dio H, Ito Y, Shibata M, Yoshinaka R, Otsuki Y. Evaluation of cell
death and proliferation in psoriatic epidermis. J Dermatol Sci 2004;35(3):207-214.
13. Mete UO, Denli YG, Ozbilgin MK, et al. Electron microscopy of psoriatic skin before and after psoralen/ultraviolet A treatment. Cutis 1996;58(1):83-86.
14. Stone OJ. Psoriasis: highly reactive early cellular inflammation. Med Hypotheses
1990;31(1):47-53.
15. Weinstein GD, McCullough JL, Ross PA. Cell kinetic basis for pathophysiology of
psoriasis. J Invest Dermatol 1985;85(6):579-583.
16. Watanabe S, Wagatsuma K, Ichikawa E, Takahashi H. Abnormal distribution of epidermal proteins antigens in psoriatic epidrmis. J Dermatol 1991;18(3):143-151.
17. Timpl R. Structure and biological activity of basement membrane proteins. Eur J
Biochem 1989.,180(3) :487-502.
18. Yurchenco PD, Cheng YS, Colognato H. Laminin forms an independent network in
basement membranes. J Cell Biol 1992;117(1):1119-1133.
19. Heng MC, Heng JA, Allen SG. Electron microscopic features in generalized pustular
psoriasis. J Invest Dermatol 1987;89(2):187-191.
20. Heng MC, Kloss SG, Kuehn CS, Chase DG. Significance and pathogenesis of basal keratinocyte herniations in psoriasis. Jinvest Dermatol 1986;87(3):362-366.
21. Aumailley M, Rousselle P. Laminins of the dermo-epidermal junction. Matrix Biol
1999 ;18(1) :19-28.
22. McGowan KA, Marinkovich P. Laminins and human disease. Microsc Res Tech
2000;51:262-279.
23. Aumailley M, Krieg T. Laminins: a family of diverse multifunctional molecules of basement membranes. J Invest Dermatol 1996;106(2):209-214.
24. Mondello MR, Califano L, Cannavo SP, et al. Psoriasis and cyclosporin: immunohistochemical aspects of the basement membrane. Acta Derm Venerol Suppl
(Stockh) 1994;186:96-98.
25. Vasil'eva Niu, Nazimova SV, Tatarinov IuS. The content of fibronectin in the blood of patients with psoriasis. Lab Delo 1991;(3):27-28.
26. O'Keefe EJ, Woodley DT, Falk RJ, Gammon WR, Briggaman RA. Production of
fibronectin by epithelium in a skin equvialent. J Invest Dermatol 1987;88(5):634-639.
27. Lauharanta J, Salo O, Kanerva L, Lassus A. The effect of etretinate on fibronectin in
psoriatic skin. Acta Derm Venereol 1982;62(4):358-361.

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