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Kutanöz mast hücrelerinden histamin salınımı üzerine ovabain'in etkisi

The Effect of Ouabain on Histamine Release from Human Skin Mast Cells

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 1996

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+-K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies utilized animal-derived cells. The aim of this study was to determine the effect of Na+-K+ ATPase inhibition on human skin mast cells. Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 fiM) for 30 min following a 1 hr incubation with various concentrations (10-4 to 10-8 M) of ouabain. Histamine release was assayed by enzyme immunoassay. The results indicated that ouabain, a Na+-K+ ATPase inhibitor, had no significant effect on the non-immunologic histamine release from human skin mast cells in vitro and suggested differences between human and animal mast cells. [Journal of Turgut Özal Medical Center 1996;3(4):277-283]
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Abstract (Original Language): 
Sodyum-potasyum adenozin trifosfataz (Na+-K+ ATPaz) inhibisyonunun, mast hücrelerinden mediatör salınımı üzerine etkileri konusunda çelişkili bilgiler mevcuttur. Bazı çalışmalar, spesifik bir Na+-K+ ATPaz inhibitörü olan ovabain (strofantin G) 'in, mediatör deşarjını bloke ettiğini, bazıları ise herhangi bir etkisinin olmadığını, hatta mediatör sekresyonunu artırdığını ileri sürmektedir. Bu çalışmaların çoğu hayvanlardan elde edilmiş mast hücreleri kullanılarak yapılmıştır. Bu çalışmanın amacı, Na+-K+ ATPaz inhibisyonunun, insan kutanöz mast hücrelerinden mediatör salınımı üzerine etkilerini incelemektir. Sağlıklı yenidoğanların sünnet derilerinden elde edilen mast hücreleri, değişik ovabain konsantrasyonlarvyla inkübe edildikten sonra, kalsiyum iyonoforla stimüle edildi. Histamin salınımı ELISA ile değerlendirildi. Sonuçlar, spesifik bir Na+-K+ ATPaz inhibitörü olan ovabain'in, insan kutanöz mast hücrelerinden in vitro nonimmünolojik histamin salınımı üzerine belirgin bir etkisi olmadığını gösterdi. İnsan ve hayvan mast hücreleri arasında bu açıdan da farklılıklar olduğu düşünüldü. [Turgut Özal Tıp Merkezi Dergisi 1996;3(4):277-283]
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  • 4
277-283
  • Turkish

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