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laktasyon ve laktasyon sonrası dönemde düşük kalsiyum diyetle beslenen sıçanların femurundaki histopatolojik değişiklikler

THE HISTOPATHOLOGICAL CHANGES IN RAT FEMURTHATWEREFED WITH LOW-CALCIUM DIET IN LACTATION AND POSTLACTATION PERIODS

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 1998

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
777e purpose of the present study was to investigate structural changes of the femur of lactating andpostlactation periods fed low-calcium diet in rats.Twenty pregnancy Wistar albino rats weighing between 200-250 gwereusedfor this study.The laboratory animals divided into two groups consist of 10 female rats.The experimental group was fed with low-calcium diet for 90 days (0.083 % Ca ).7he puppy rats in this group following lactation and postlactation periods were fed with same diet The control group was fed with standard diet (Standard pellet-0.5%Ca). At the end of the experimental feeding period, the animals were sacrified and their femurs, removed enbloc sections (5mm) were taken and stained (Hematoxylen-Eosin, Masson Trichrome, Safranin fast green) for the light microscope. Light microscope examination; in the puppy rats in low-calcium diet group some histological sections of bone showed deminerilization area, an irregularitiy of inner surface of the lamellae and increase of the number of osteoclasts. Reducing the thickness of bone cortex and spongiosae trabeculae result from resorbtion were noted
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Abstract (Original Language): 
Çalışmamızda laktasyon ve laktasyon sonrası düşük kalsiyum diyetle beslenen ratların femur kemiğindeki histolojik değişiklikleri araştırdık. Araştırmamızda 20 tane erişkin 200-250 g ağırlığındaki Wistar albino rat kullanıldı. Kontrol grubundaki ratlar çiftleştirmeye alındıkları günden itibaren standart pelet yem ile beslendi (%0,5 Ca içeren).Bu gruptanelde edilenratlar, laktasyon ve laktasyon sonrası periyotta da aynı diyetle beslendi. Deney grubundaki dişi ratlar, çiftleştirmeye alınarak gebelik döneminde standart pelet yem ile beslendi (%0,5 Ca içeren). Deney grubunun yavru ratları laktasyon ve laktasyon sonrası düşük kalsiyum içeren ( %0 083 Ca içeren ) diyetle beslendi Deney hayvanları 90 günde eter aneztezisi altında sakrifiye edilerek sağ femurları alınarak, %10'luk nötral formalinde fikse edildi. Femurların dekalsifikasyonu %5'lik formik asitle yapılarak, rutin histolojik takiplerle elde edilen 5— kalınlığındaki parafin kesitleri Hematoxylen-Eosin, Masson trichrome Safraninfastgreen ileboyandı. Yavru ratların femur kesitlerinde; kompakt kemikte deminerilizasyon alanları ve lamellerde düzensizlikler ile osteoklast sayısında artış gözlendi.Rezorpsiyon sonucu kemikkorteksivespongiöztrabeküllerdebelirginbirincelmesaptandı.
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  • 2-3
114-117
  • Turkish

REFERENCES

References: 

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Turgut Özal Tıp Merkezi Dergisi 5(2,3):1998
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