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Melatoninin Superoksit Yakalama Kapasitesinin In Vitro incelenmesi

AN ASSAY FOR THE IN VITRO DEMONSTRATION OF SUPEROXIDE SCAVENGING CAPACITY OF MELATONIN

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2000

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Aim: In vitro assay of superoxide scavenging capacity of melatonin. Materials and methods: Scavenging capacity of melatonin for superoxide radicals produced by xanthine-xanthine oxidase system was investigated by using Fridovich I. method. Results and discussion: Melatonin exhibited superoxide scavenging activity in a dose-dependent manner up to 45 pg/mL and activity was calculated to be 0.046 ±0.009 SOD equivalent units / pg of melatonin (n=9). A concentration of approximately 18 pg / mL melatonin served as one unit of superoxide dismutase enzyme.
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Abstract (Original Language): 
Amaç: Melatoninin radikal süpürücü kapasitesinin in vitro koşullarda incelenmesi amaçlandı. Materyal ve metot: Ksantin-ksantin oksidaz sisteminde oluşturulan superoksit radikallerinin melatonin tarafından süpürüldüğü sitokrom c kullanılarak Fridovich I. yöntemi ile araştırıldı. Bulgular ve sonuç: Çalışma bulgularımız melatoninin in vitro koşullarda da superoksit radikallerine karşı anlamlı ölçüde temizleyici etkiyi gösterdiği yönündedir.
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  • 3
190-193
  • Turkish

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