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Patoloji Laborantlarında Ksilen Solumanın Antioksidan Sistem Üzerine Etkileri

The Effects Of Xylene Inhalation On The Antioxidant System Of Pathology Lab Workers

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Abstract (2. Language): 
Organic compounds, which are used as solvents in many areas, threaten human health. In this study, the antioxidant system of pathology lab workers who use xylene and xylole during hematoxylene dying and tissue pursuit processes were investigated. The bloods from 12 lab workers (7 men and 5 women) and 12 healthy volunteers (7 men and 5 women) were used for this study. For this purpose, SOD, CAT activities and MDA levels in the RBC and NOx levels, ALP, AST, ALT activities were measured in the serum of each individual. All of the analyses were done using photometric methods. Results were as follows; serum ALP (135 ±14 U/L), AST (21±4 U/L) and ALT (19±3 U/L) levels of lab workers were found in the normal range and NO x levels were found 44,6±12,0 pmol/L (control: 26,1±2,8 pmol/L). SOD, CAT activities, MDA levels in the RBC were 1667,7±161,8 U/gHb (control: 2359,4±223,6 U/gHb); 99,7±14,3 k/gHb (control: 221,0±12,3 k/gHb); 282,7±32,4 nmol/ml (control: 145,3±17,2 nmol/ml) respectively. SOD and CAT activities were lower than controls (p<0.05). MDA and NOx levels were significantly higher than controls (p<0.05) and ALT, AST, ALP activities were within the normal ranges. Our results indicate that antioxidant defense systems of pa thology lab workers have decreased.
Abstract (Original Language): 
Günümüzde pek çok alanda çözücü olarak kullanılan organik bileşikler insan sağlığını ciddi boyutlarda tehdit etmektedir. Bu çalışmada patoloji laboratuvarlarında hemotoksilen boyama ve doku takibi işlemleri sıras ında ksilen ve ksilol kullanan laborantların antioksidan sistemleri araştırıldı. Çalışmada 7'si erkek 5'i kadın toplam 12 laborant ve 12 sağlıklı bireyden alınan kanlar kullanıldı. Bu amaçla söz konusu laborantların serum ALP, AST, ALT ve NOj düzeyleri ile eritrosit içi SOD, CAT ve MDA düzeyleri ölçüldü.Tüm ölçümler fotometrik yöntemlerle gerçekleştirildi ve sonuç olarak serum ALP(135 ±14 U/L), AST(21±4 U/L) ve ALT(19±3 U/L) değerleri normal sınırlar içerisinde bulunurken NOx düzeyi 44,6±12,0 pmol/L (kontrol: 26,1±2,8pmol/L) olarak bulundu. Eritrosit içi ölçümlerde ise sırasıyla MDA, SOD ve CAT düzeyleri 282,7 ±32,4 nmol/ml, 1667,7±161,8 U/gHb, 99,7±14,3 K/gHb olarak bulunurken bu parametreler kontrol için yine aynı sıra ile 145,3 ±17,2 nmol/ml, 2359,4±223,6 U/gHb ve 221,0±12,3 K/gHb şeklinde tespit edilip yapılan istatistiki değerlendirme sonucunda NOj ve MDA düzeyleri kontrole kıyasla anlamlı bir şekilde artmış (p<0,05); SOD ve CAT aktivitelerinde ise yine kontrole kıyasla anlamlı bir azalma olduğu gözlenmiş tir (p<0,05).Çalışma bulgularımız patoloji laborantlarının antioksidan savunma sistemlerinin zayıfladığı yönündedir.

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