You are here

Home » Content

An experimental study on effect of antioxidant vitamin E in stress and alcohol induced changes in male fertility in albino rats

An experimental study on effect of antioxidant vitamin E in stress and alcohol induced changes in male fertility in albino rats

Journal Name:

  • International Journal of Medical Research & Health Sciences

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
Introduction: Physical and Psychological stresses are believed to reduce sexual functions, resulting from neurotransmission changes in various erectile response pathways and reduced blood flow in genital organs. Intake of alcohol depends on numerous genetic and environmental factors. Stress has long been thought to influence the alcohol drinking in humans. Forced swimming in laboratory animals has been widely used as a model of stress to study the physiological changes and the capacity of the organism in response to stress. Aim: 1. To assess the effect of forced swimming stress on seminal fluid profile, Serum testosterone level, Testicular lipid peroxidation levels. 2. To assess the effect of Vitamin E on stress induced changes. Method: Adult male albino rats weighing 200 – 220 g, aged 12-15 weeks were used in this study. The animals were randomly divided into four groups of 6 animals each. Group1 (control) received distilled water, Group 2 (Forced Swimming Stress) received distilled water Group 3: subjected to Forced Swimming stress with 20% alcohol intake p.o. Group 4: Swimming stress with 20% alcohol treated with Vitamin E (200mg/kg/day orally). The following parameters were studied in all groups 1. Body weight. 2. Sperm count Motility and Life /death Ratio (SLDR). 3. Serum Testosterone 4. Testicular Malondialdehyde level (MDA). Results: Forced swimming stress caused loss in body wt, reduction in sperm count, motility and SLDR in sperm analysis, reduction in serum testosterone and increase testicular MDA levels compared to control. All the changes were statistically significant. When alcohol was added along with forced swimming it caused further loss in body wt, sperm count, motility and SLDR, serum testosterone level and slightly further increase in MDA levels. These observations were also statistically significant. In case of group IV in which Vitamin E was administered long with stress with alcohol it showed a trend of reversal phenomenon of stress and alcohol induced change (Group III) exhibited by gain in body wt, increase sperm count, motility and SLDR, serum testosterone and decrease in MDA level. All the results were statically significant except serum testosterone. Conclusion: Force swimming indicates that swimming is an effective model for producing stress in albino rats. The stress induced changes were further accentuated by addition of ethyl alcohol. However Vit E treatment reverses the effect of stress and alcohol.
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon arastirmax-experimental-study-effect-antioxidant-vitamin-e-stress-and-alcohol-induced-changes-male-fertility-albino-rats.pdf
  • 1
1-6
  • Turkish

REFERENCES

References: 

1. Bhatia N, Maiti PP, Choudhary A, Tuli A and
Masih D.Animal models and stress. Int. J. Pharm.
Sci. Res. 2010;2: 1147-55
2. Nayanatara AK, Nagaraja HS and Anupama BK.
The effect of repeated swimming stress on organ
weights and lipid peroxidation in rats. Thai Journal
of Phsyiological Sciences.2005;18(1):3-9
3. Yazawa H, Sasagawa I, Ishigooka M and Nakada
T, Effect of immobilization stress on testicular germ
cell apoptosis in rats. Hum. Reprod., 1999: 14:
1806-10.
4. Sikka SC, Rajasekaran M and Hellstrom WJ. Role
of oxidative stress and antioxidants in male
infertility. J. Androl.1995;16: 464-68.
5. Saki G, Rahim F and Vaysi OA. Effect of forced
swimming stress on vivo fertilization capacity if rat
subsequent offspring quality. J Hum Reprod Sci.
2010;3(1):32-34.
6. Mallikarnjunarao B, Chandrashekar M, Vijay Prasad
S, Kate NN, Kondam A and Suresh M . A study of
effect of stress on voluntary alcohol intake in Wistar
rats on behavioral changes. Int J Biol Med Res.
2012;3: 1612-15.
7. Yadav SB, Suryakar AN, Huddedar AD, Durgawale
PP and Shukla PS. Antioxidant treatment a new
therapeutic approach to reversible male infertility.
Biomed Res.2006;17: 175-78.
8. Du Plessis SS, Kashou A, Vaamonde D and
AgarwalA. Is there a link between excise and male
factor fertility? Open Reprod. Sci. J.2011; 3: 105-13
9. Sharma RK. and Agarwal A. Role of reactive
oxygen species in male infertility. Urology.1996; 48:
835-50.
6
10. Agarwal A, and Saleh A. Role of oxidants in male
infertility: rationale, significance and treatment.
Urologic Clinics of North America. 2002;29(4)
November.
11. Kini RD, Nayanatara AK, Pai SR, Ramswamy C,
Bhat MR and ManturVS. Infertility in male wistar
rats induced by cadmium chloride : role of ascorbic
acid. Journal of Chinese Clinical Medicine.
2009;4(11):616-21
12. Musalmah M, Fairuz AH, Gapor MT and Wan WZ,
Ngah Effect of Vitamin E on plasma
malondialdehyde, antioxidant enzyme levels and the
rates of wound closures during wound healing in
normal and diabetic rats. Asia Pac. J. Clin. Nutr.
2002; 1: S448-51.
13. Aitken RJ and RomanSD. Antioxidant systems and
oxidative stress in the testes. Oxid. Med. Cell
Longev. 2008;1: 15-24.
14. Bairy L, Paul V and Rao Y, Reproductive toxicity
of sodium valproate in male rats. Indian J.
Pharmacol., 2010:42: 90-94.
15. Zhang DR, Wan Y, Zu J, Zhan JG, Li L and Bai JQ.
Morinda Officinalis how enhances exercise
endurance and possesses protective effects against
oxidative stress of the rats after exercise. Afr. J.
Microbiol. Res., 2010;4: 1609- 15.
16. Metwally SA, Abd HA, El-Latif, Rawzy HM and
Hamdy A. The protective effect of linseed oil
against carbendazim induced testicular toxicity in
rats. Eur. J. Sci. Res.2011; 49: 208-24
17. Rai J, Pandey SN and Srivastava RK. Testosterone
hormone level in albino rats following restraint
stress of long duration. J. Anat. Soc. India.
2004;53: 17- 19
18. El-Shahat AER, Gabr A, Meki AR and Mehana ES.
Altered testicular morphology and oxidative stress
inducedby cadmium in experimental rats and
protective effect of simultaneous green tea extract.
Int. J. Mrophol.2009;27: 757-64.
19. Abdel Moniem AE, Dkhil MA and Al- Quraishy S,
Protective role of flaxseed oil against lead acetate
induced oxidative stress in testes of adut rats.
Afirican journal of Biotechnology. 2010;
9(42):7216- 23.
20. Patil L. and R. Balaraman. Effect of melatonin on
doxorubicin induced testicular damage in rats. Int. J.
PharmTech Res.2009; 1: 879-84.
21. Saalu LC, Kpela T, Benebo AS, Oyewopo AO,
Anifowope EO and Oguntola JA. The dosedependent
testiculoprotective and testiculotoxic
potentials of Telfairia occidentalis Hook f. leaves
extract in rat. Int. J. Applied Res. Nat. Prod. 2010;3:
27-38.
22. Nashwa AA, Kawkab AA and Mouneir SM. The
protective effect of ginger and N-acetyl cysteine on
ciprofloxacin-induced reproductive toxicity in male
rats. J. Am. Sci. 2011;7: 741-52.
23. Mingoti GZ, Pereita and Monteiro CMR. Fertility
of male adult rats submitted to forced to forced
swimming stress. Brazilian Journal of Medical and
Biological Research. 2003;36: 677-78

Thank you for copying data from http://www.arastirmax.com