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DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF SILDENAFIL CITRATE AND DAPOXETINE HYDROCHLORIDE IN THEIR COMBINED TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
The present manuscript describes new, simple, accurate, and precise high performance thin layer chromatography method for the simultaneous determination of Sildenafil Citrate and Dapoxetine Hydrochloride in combined tablet dosage form. Chromatographic separation of the drugs was performed on aluminium plates are coated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Chloroform: Methanol: Acetonitrile : Glacial acetic acid (5:1:2.5:1.5 v/v). Densitometric evaluation of the separated zones was performed at 292 nm. The two drugs were satisfactorily resolved with Rf values 0.82 and 0.56 for Sildenafil Citrate and Dapoxetine Hydrochloride respectively. The linear regression data for the calibration plots showed good relationship with r2 = 0.993 from 500-1500 ng/spot for Sildenafil Citrate and r2 = 0.995 from 1000-3000 ng/spot for Dapoxetine Hydrochloride. The methods were validated for precision, accuracy, and recovery. The percentage recovery for Slidenafil Citrate was found to be 100.02% – 100.08% and 99.95% – 99.99%.Dapoxetine Hydrochloride. The limits of detection and quantification were 143.3625 and 434.4318 ng/spot per spot for Sildenafil Citrate and 315.4586 and 955.9351 ng/spot per spot Dapoxetine Hydrochloride, respectively.
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  • English

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