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CYTOTOXIC, APOPTOTIC AND ANTI-ANGIOGENIC EFFICACY OF SILVER NANOPARTICLES, SYNTHESIZED USING TANNIC ACID AS REDUCING AGENT UNDER CONTROLLED TEMPERATURE.

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Abstract (2. Language): 
Silver nanoparticles has been previously used as antimicrobial and antiviral agent .The present study reveal the cytotoxic, apoptotic and anti-angiogenic efficacy of temperature dependent synthesized silver nanoparticles by using tannic acid as reducing agent. MCF [breast cancer cell] were treated with silver nanoparticles (ranged from 100nm to 800nm/ml) for different time period at 37°C and 5% CO2 atmosphere. Cell Viability was evaluated by MTT assay and the mechanism of cell death [Apoptosis] through Ethidium bromide/Acridine orange and DNA laddering assay. Anti-angiogenic properties were determined by cell migration assay. Silver nanoparticles showed dose-dependent cytotoxic, apoptotic and anti angiogenic effect in MCF-7 breast cancer cells at an 400nM/ml. The present study showed that silver nanoparticles may be an alternative chemotherapeutic agent for treatment of cancer.
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Available Online At www.ijprbs.com
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