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DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND LEVOFLOXACIN HEMIHYDRATE IN THEIR COMBINED TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
Two simple, accurate, precise and economical spectrophotometry methods have been developed for the estimation of Cefixime trihydrate (CEF) and Levofloxacin hemihydrates (LEVO) simultaneously in tablet dosage form. Simultaneous method (Method 1) and Absorbance Ratio (Q-Absorbance) method (Method 2) were used. The wavelength ranges 240.27 nm and 296.28 nm in Simultaneous method were selected to determine Cefixime trihydrate (CEF) and Levofloxacin hemihydrate (LEVO), respectively and wavelength ranges 289.39 nm (iso-absorptive point) and 240.27 nm (Amax of Cefixime trihydrate) were selected for Absorbance ratio (Q-Absorbance) method. Beer's law is obeyed in the concentration ranges of 3-15 ug/ml and 3-15 ug/ml for Cefixime trihydrate(CEF) and Levofloxacin hemihydrate(LEVO) for Simultaneous method as well as Absorbance ratio method. The % assay for commercial formulation was found to be in the range 99.92% - 100.07% for Simultaneous method and 99.85-100.04 % for Absorbance Ratio by the proposed methods. Recovery was found in the range of 98.33-101.25% for Cefixime trihydrate and 98.66-101 % for Levofloxacin hemihydrate by Simultaneous spectroscopic method and 98.33-100.75 % for Cefixime trihydrate and 98.66 -99% for Levofloxacin hemihydrate by Absorbance ratio method for both the Formulations.
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  • 5
502-515
  • English

REFERENCES

References: 

1. Genvresse I and Carbon C: Cefixime Trihydrate. International Journal of Antimicrobial Agents, 1993; 3:1-16.
2. Indian Pharmacopoeia Govrnment of India, Ministry of Health & Welfare, 6th edition 2010; 2:857-858
3. The British Pharmacopoeia. London: Her Majesty's Stationery Office, 1998.
4. United States Pharmacopoeia. 30th ed., United States Pharmacopeial Convention, RockVille.2007.
5. Sweetman S. C. and Martindale: The Complete Drug Reference; 33rd ed., Pharmaceutical Press: London, 2002; 219.
6. Fera M, Carbone M and Foch A: Activity of Cefixime Trihydrate against Helicobacter pylori. International Journal of Antimicrobial Agents, 1993; 3:105-108
7. Beckett A. H. and Stenlake JB: Practical Pharmaceutical Chemistry, 4th Edition, Part II, CBS Publications and Distributors, New
Delhi, 1997; 1: 275-300
8. Shah P.B. and Pundarikakshudu K: Spectrophotometric, Difference Spectroscopic and High-Performance Liquid Chromatographic Methods for the Determination of Cefixime in Pharmaceutical Formulations, J. AOAC Int.,2006; 89: 987-994.
9. Al-Momani IF: Spectrophotometric determination of selected cephalosporins in drug formulations using flow injection analysis. Journal of Pharmaceutical and Biomedical Analysis. 2001; 25: 751-57.
10. Arshad HM, Gauhar S, Bano R and Muhammad IN: Development of HPLC-UV method for analysis of cefixime in raw materials and capsule. Jordan journal of pharmaceutical sciences. 2009; 2(1): 53-65.
11. Falkowski A, Look Z, Noguchi H and Silber M: Determination of Cefixime Trihydrate in biological samples by Reversed-Phase High-Performance Liquid Chromatography. Journal of Chromatography, 1987; 422:145-152.
C
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Research Article
Payal Patel, IJPRBS, 2012; Volume 1(5): 502-515
12. Maheshwari R.K., Kinariwala M., Saxena M., Gahlot M., Chaki R. and Jagwani Y: Spectrophotometric Analysis of Cefixime Trihydrate Tablets using Metformin Hydrochloride as Hydrotropic Solubilizing Agent, Asian J. Chem., 2008; 20: 6047-6050.
13. Zendelovsk D., Stafilov T and Milosevski P:High-performance liquid chromatographic method for determination of cefixime and cefotaxime in human plasma. Bul.Chem.Tech., 2003; 22(1): 39-45
14. Prabhu S., Vijay Amirtharaj R. and Senthilkumar N. (2010). Simultaneous RP-HPLC Method Development and Validation of Cefixime and Ofloxacin in Tablet Dosage Form. Asian J. Research Chem
15. Janhavirao, Kaminisethy And Savitayadav: Validated hptlc method for simultaneous quantitation of cefixime and ofloxacin in bulk drug and in pharmaceutical formulation" , Int.J.Pharm.Res, 2010; 02: 42-45.
16. El-Brashy AM, Metwally ME and El-Sepai FA: Spectrophotometric determination of Some Fluoroquinolone Antibacterials through Charge-transfer and Ion-pair
ISSN: 2277-8713
IJPRBS
Complexation Reactions. Bull. Korean Chem. Soc., 2004; 25(3): 365-372
17. Burhenne J, Ludwig M and Spiteller M: Polar. photodegradation products of quinolones determined by HPLC/MS/MS. Chemosphere. 1999; 38: 1279-86.
18. Oliphant CM and Green GM: British Pharmacopoeia. Department of Quinolones: A Comprehensive review. Am Health. London: HMSO Publication; Fam Physician February 2002; 65: 455-64.
19. Bottcher S, Baum HV, Hoppe-Tichy T, Benz C and Sonntag HG: An HPLC and microbiological assay to detertmine levofloxacin in soft tissue, bone and serum. J. Pharm. Biomed. Anal., 2001; 25: 197.
20. Shirkhedkar AA and Surana SJ: Quantitative Determination of Levofloxacin Hemihydrate in Bulk and Tablets by UV-Spectrophotometry and First Order Derivative Methods. Pak J Pharm Sci 2009; 22: 301-02.
21. Belal F, Al-Majed AA and Al-Obaid AM: Methods Analysis of 4-Quinolone Antibacterials. Talanta 1999; 50: 765-86.
Available Online At www.ijprbs.com
Research Article
Payal Patel, IJPRBS, 2012; Volume 1(5): 502-515
22. Wong a FA, Juzwin SJ and Flor SC: Rapid stereospecific highperformance liquid chromatographic determination of levofloxacin in human plasma and urine. J. Pharm. Biomed. Anal 1997; 15: 765-771.
23. Meyyanathan SN, Ramasarma GVS and Suresh B: Analysis of levofloxacin in pharmaceutical preparations by high performance thin layer chromatography. J. Seperation Sci., 2003; 26: 1698-1700.
ISSN: 2277-8713
IJPRBS
24. Kothekar KM, Jayakar B, Khandhar AP and Mishra RK: Quantitative Determination of Levofloxacin and Ambroxol hydrochloride in Pharmaceutical Dosage Form by Reversed-Phase High Performance Liquid Chromatography. Eurasian J Anal Chem 2007; 2: 21-31.
25. Validation of analytical procedure, Methodology, ICH Harmonized Tripartite Guidelines. 1996: 1-8.
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