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METHOD DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF CEFIXIMETRIHYDRATE AND LINEZOLID IN THEIR COMBINED TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

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Abstract (2. Language): 
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of cefixime Trihydrate (CEF) and Linezolid (LNZ) in combined tablet dosage form. The method is based on the Q analysis method and Simultaneous equations for analysis of both the drugs using methanol as solvent. First method is Q analysis method based on absorbance ratio at two selected wavelengths 278.72nm (iso-absorptive point) and 256.70nm (Amax of linezolid).Second method is based on the simultaneous equations and wavelengths selected for analysis were 288.72 nm (Amax of Cefixime Trihydrate) and 256.70nm (Amax of Linezolid) respectively, in methanol. The linearity was obtained in the concentration range of 2-10 ug/ml and 5-25 ug/ml for both Cefixime Trihydrate and Linezolid. The results of the analysis have been validated statistically and by recovery studies. Method was found to be accurate, precise and reproducible. This method was applied to the assay of the drugs in marketed formulation, which were found in the range of 99.70% to 100.03% of the labeled value for both Cefixime Trihydrate and Linezolid in Q analysis method and 100.10% and 99.91% of the labeled value for both Cefixime Trihydrate and Linezolid in Simultaneous equation method. Hence, the methods herein described can be successfully applied in quality control of combined pharmaceutical dosage forms.
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  • 5
516-529
  • English