You are here

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF ALOE VERA

Journal Name:

Publication Year:

Abstract (2. Language): 
The Aim of this study was to investigate the hepatoprotective effect of aqueo extract of Aloe vera against carbontetrachloride induced hepatotoxicity in rats. 36 Albino wistar rats (150 normal saline (2ml/100g orally), II group received 1 ml/kg of carbontetrachloride intraperitoneally (i.p.), III group was given Liv.52 (2 ml/kg) orally for ten days followed by carbontetrachloride i.p. on 10 (2ml/100g/day) for 10, 20 & 30 days respectively followed by carbontetrachlorid on 10 rats were anaesthetized with ketamine (75 mg/kg) and diazepam (10 mg/kg). Blood was collected & then animals were sacrificed to take out the liver for histopat examination. The sera were used to estimate alanine aminotransferase, alkaline phosphatase & total billirubin and liver were subjected to histopathological examination. Alkaline phosphatase, alanine transaminase & total billirubin level in normal increased in carbon tetra chloride treated group. Aloe vera extract displayed significantly low values of alkaline phosphatase, alanine aminotransferase & billirubin in group III, IV It can be concluded that Aloe vera extract is a potent hepatoprotective agent.
FULL TEXT (PDF): 

REFERENCES

References: 

1. Karan M, Vasisht K, Handa SS.
Antihepatotoxic activity of Swertia
chirataon carbon
tetrachlorideinducedhepatotoxicity in rats.
Phytotherapy Research 1999; 13: 24–30.
2. Chaterrjee TK. Medicinal Plants with
Hepatoprotective Properties. Herbal
Options. Books and Applied Allied (P) Ltd.,
Calcutta 2000; 143.
3. http://www.himalayahealthcare.com/res
earchpaper/liv52.htm
Research Article ISSN: 2277-8713
Ravi Kant, IJPRBS, 2013; Volume 2(1): 324-341 IJPRBS
Available Online At www.ijprbs.com
4. http://www.karinya.com/liver.htm
5. Wixson SK, White WJ. A comparison of
Pentobarbital, Fentanyl-Droperdiol,
Ketamine-Xylazine and Ketamine-Diazepam
Anesthesia in Adult Male Rats. LAS 1987;
37: 726-730.
6. Reitman S, Frankel S, Am J. Clin Pathol
1957; 28: 56-63.
7. Kind PRN, King EJ. Estimation of plasma
phosphatase by determination of
hydrolysed phenol with amino-antipyrine.
Journal of clinical Pathology 1954;7: 322.
8. Tietz NW. Fundamentals of Clinical
Chemistry. WB Saunders Company 1986;
1388-1390.
9. Ghosh MN. Fundamentals of
experimental pharmacology. 4th ed. Kolkata:
Hilton & Company 2008; 235-237, 273.
10. Talib VH. A handbook of medical
laboratory technology. 2nd ed. New Delhi:
CBS Publishers & Distributors 2007; 155-
167.
11. Clayden EC. Practical Section Cutting and
Staining - Part 1, London: Churchill
Livingstone 1971; 69.
12. Slater TF. Biochemical mechanism of liver
injury. London Academic Press 1965.
13. Recknagel RO, Glende RA, Hruszkewycz
AM. In: Pryor, E. A. Jr. (Ed.), Free Radicals in
Biology, Vol. II. Academic Press NY 1976; 97-
132.
14. Recknagel RO. A new direction in the
study of carbon tetrachloride
hepatotoxicity. Life Science 1983; 33: 401-
408.
15. DeGroot H, Noll T. The crucial role of low
steady state oxygen partial pressures in
haloalkan free-radical mediated lipid
peroxidation. Biochemical Pharmacology
1986; 35: 15-19
16. Recknagel RO, Glende EA. Carbon
tetrachloride hepatotoxicity: an example of
lethal cleavage. Critical Review Toxicology
1973; 2: 263-297.
17. Gravel E, Albano E, Dianzani MU, Poli G,
Slater TF. Effects of carbon tetrachloride on
isolated rat hepatocytes: Inhibition of
protein and lipoprotein secretion”.
Biochemical Journal 1979; 178: 509-512.
18. Wolf CR, Harrelson WG, Nastainczyk
WM, Philpot RM, Kalyanaraman B, Mason
Research Article ISSN: 2277-8713
Ravi Kant, IJPRBS, 2013; Volume 2(1): 324-341 IJPRBS
Available Online At www.ijprbs.com
RP. Metabolism of carbon tetrachloride in
hepatic microsomes and reconstituted
monooxygenase systems and its
relationship to lipid peroxidation. Molecular
Pharmacology 1980; 18: 553-558.
19. Azri S, Mat, HP, Reid LL, Gandlofi AJ,
Brendel K. Further examination of the
selective toxicity of CCl4 rat liver silices.
Toxicology and Aplied Pharmacology 1992;
112: 81-86.
20. Visweswaram B, Rao PR, Satyanarayan S.
A non invasive method for screening
hepatoprotective drugs against carbon
tetrachloride induced hepatotoxicity”.
Indian Journal of Pharmacology 1994;
26:301-303
21. Jayasekhar P, Mohanan PV, Rathinam K.
Hepatoprotective activity of ethyl acetate
extract of Acacia catechu. Indian Journal of
Pharmacol 1997; 29: 426-428.
22. Kulcsar GJ, Kulcsar A. Studies on the
effect of ursodesoxycholic acid on rats with
acute carbonte trachloride injury.
Arzneimittel-Forschung 1997; 47: 659-661.
23. Gumucio JJ. Hepatocyte heterogeneity:
The coming of age from the description of a
biological curiosity to a partial
understanding of its physiological meaning
and regulation. Hepatology 1989; 9: 154.
24. Weber LWD, Boll M, Stampfl A.
Hepatotoxicity and mechanism of action of
haloalkanes: Carbon tetrachloride as a
toxicological model. Critical reviews in
toxicology 2003; 33: 105-136.
25. Wroblewski F, La Due JS. Serum glutamic
oxaloacetic transaminase activity as an
index of liver cell injury from cancer. A
preliminary report Cancer 1955;8:1155-
1163.
26. Molander DW, Sheppard E, Payne MA.
Serum transaminase in liver disease. J. Am.
Med. Assoc 1957; 163: 1461-1465.
27. Zelman S, Wang CC, Appelhanz I.
Transaminases in needle aspiration
biopsies. Am. J. Med. Sci 1959; 237: 323-
333.
28. Boudreau MD, Beland FA. An evaluation
of the biological and toxicological
properties of Aloe barbadensis (miller), Aloe
vera. Journal of environmental science and
health. Part C, Environmental
carcinogenesis & ecotoxicology reviews
2006; 24: 103–105.
Research Article ISSN: 2277-8713
Ravi Kant, IJPRBS, 2013; Volume 2(1): 324-341 IJPRBS
Available Online At www.ijprbs.com
29. King GK, Yates KM, Greenlee PG. The
effect of Acemannan Immunostimulant in
combination with surgery and radiation
therapy on spontaneous canine and feline
fibro sarcomas. Journal of the American
Animal Hospital Association 1995; 31: 439–
447.
30. Eshun K, He Q. Aloe vera: a valuable
ingredient for the food, pharmaceutical and
cosmetic industries--a review. Critical
reviews in food science and nutrition 2004;
44: 91–96.
31. Muriel P, Mourelle M. Prevention by
silymarin of membrane alterations in acute
CCl 4 liver damage. J Appl Toxicol 1990; 10:
275-279

Thank you for copying data from http://www.arastirmax.com