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PHYTOCHEMICAL SCREENING, ENZYME AND ANTIBACTERIAL ACTIVITY ANALYSIS OF ENDOPHYTIC FUNGI BOTRYTIS SP ISOLATED FROM FICUS BENGHALENSIS

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2013

Key Words:

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Abstract (2. Language): 
The present study deals with phytochemical analysis, enzyme properties and antibacterial activity analysis of endophytic fungi Botrytis sp isolated from aerial root of Ficus benghalensis. An endophytic fungus was isolated by using PDA (Potato Dextrose Agar) medium. Qualitative analysis of the phytochemical was performed following generally accepted laboratory techniques; so also the different enzymes produced from the fungus were analyzed. The antibacterial activity of isolated endophytic fungi was tested against one gram positive bacteria namely Staphylococcus aureus and two gram negative bacteria namely Escherichia coli and Klebsiella sp adopting well diffusion method. The qualitative phytochemical analysis of the endophytic fungi showed positive results for alkaloids, flavonoids, terpenoids, steroids, alkaloids and saponins. (Except phenols, cardiac glycosides). The fungal culture has the ability to produce amylase and laccase enzymes. (Except cellulase, lipase and protease). The crude fungal extract showed inhibition only of Escherichia coli (13mm) and Klebsiella sp (15 mm). Streptomycin used as a control showed inhibition zone range of 25 to 30 mm. The present study recorded that endophytic fungi Botrytis sp produce bioactive compounds. Further studies on characterization, in vitro and in vivo studies, would reveal the antibacterial activity can be performed to see the activity of this endophytic fungus in clinical trials against different human ailments.
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REFERENCES

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