You are here

Home » Content

Pharmacological Bioactivity Honey Bee Venom Apis Nigrocincta Smith and Apis Dorsata Binghami Endemic to North Sulawesi

Journal Name:

  • International Journal of Science and Engineering Investigations

Publication Year:

  • 2013

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
The aims of this research are to find the venom composition and pharmacologic bioactivity of A. nigrocincta Smithand A. dorsata Binghami honey bee which are endemic of Sulawesi, including antibacterial, antioxidant, and anticancer activity. To identify the venom composition, the SDS PAGE method was applied. Antibacterial activity was assessed using agar diffusion method. Antioxidant activity was assessed by TBA method, while anticancer activity was examined using cytotoxic method in leukemia P388 cell line. The results showed that the BV1 (Apis dorsata Bighami) has 4 molecular bands of 33.54 kDa, 32.21 kDa, 21.51 kDa, 6.7 kDa, and 2.88 kDa, while DV2 (Apis nigrocincta Smith) has 4 peptide bands detected as follows: 33.54 kDa, 21.51 kDa, and 15.43 kDa. It was suspected that BV1 contains hyaluronidase/phospholipase A, phospolipase, lysophos-pholipase or antigen 5, protease inhibitor, and melitin. BV2 was suspected to contain hyaluronidase / phospholipase A, lysophospholipase or antigen 5. BV1 had a stronger antibacterial activity compared to BV2 towards E. cole cc and S. aureus cc isolated from infected wounds. The average BV1 antibacterial activity was higher than the activity of 50 ug/mL Ampicilin as positive control. BV1 antioxidant activity was stronger than BV2, in terms of the inhibition of MDA formation at concentration 500 ug/mL (80.78% vs. 75.10%, respectively). The anticancer activity of BV1 was also better than BV2 in term of IC50 (46.77 ug/mL and 66.2 ug/mL, respectively).
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon ijsei-21813-03.pdf
  • 18
25-33
  • English

REFERENCES

References: 

[1] Hassanein NMA and Hegab Am. 2010. Bee Venom – Lead Acetate Toxicity Interaction.Australian Journal of Basic and Applied Sciences 4 (8) : 2206-2221.)
[2] Wan IP S, Wei Chuan H, Lin Pin J, Kuo Man H, Liu Ching K, Hsu Chun S, Yang Sing J, Yang Due M, Chiu Hung T, Han Mi, S and Chung Gung J. 2008. Bee Venom Induced Cell Cycle Arrest and Apoptosis in Human Cervical Epidermoid Carcinoma Ca Ski Cells. Anticancer Research March-April 2008 vol. 28 no. 2A 833-842
[3] Huha Eun J, Hyeon Baekb Y, Hoo Leec, M, Young Choic, D, Suk Parkb D and Doong Leec, J. 2010. Bee venom inhibits tumor angiogenesis and metastasis by inhibiting tyrosine phosphorylation of VEGFR-2 in LLC-tumor-bearing mice.Cancer LettersVol. 292,Issue 1, Pages 98-110.
[4] NCBI, 2010. Melitten Compound Summary PubChem. http://www.pubchem.ncbi.nlm.nih.gov[di akses 7 april April 2011].
[5] Chmielewska HR and Szczêsna T. 2004. HPLC study of chemical composition of Honeybee (Apis mellifera L.) Venom. Journal of Apicultural Science. Vol. 48 No. 2 2004
[6] Hadisoesilo S. 1997. A comparative study of two spesies of cavity-nesting honey bees of Sulawesi, Indonesia. [Dissertation] The University of Guelph, Canada.
[7] Kikuzaki H, Nakatani N. 1993. Antioxidant effects of some ginger constituents. J Food Sci 58:1407-10
[8] Mokosuli YS. , 2008. Antioxidative and anticancer activity of the stem bark extract Langsat (Lansium domesticum L.) [thesis] Graduate School of Bogor Agricultural University.
[9] Mokosuli YS, Worang RL dan Sumampouw H. 2010 Panduan Praktikum Mikrobiologi. Jurusan Biologi FMIPA Universitas Negeri Manado.
[10] Kim Te S, Hwang JY, Sung Suk M, Je Yang. S. bae Rok D. Mi Han S and Lee Hae S. 2009. The minimum inhibitory concenteration (MIC) of bee venom against bacteria isolatd from Pigs and Chikens. Korean J. Vet. Serv. 29(1) : 19-26.
[11] Surendra NS, Jarayam GN and Reddy MS. 2011. Antimicrobial activity of crude venom extracts in honeybees (Apis cerana, Apis dorsata, Apis florea) tested against selected pathogens. African Journal of Microbiology Research. Vol. 5 (18) pp 2765-2772.
[12] Behbahani M, Ali AM, Muse R, Mohd NB. 2007. Anti-oxidant and anti-inflamatory activities of leaves of Barringtonia racemosa. J Med Plant Res 96-102
[13] Tukozkan N, Erdamar H, Seven I. 2006. Measurement of total malondialdehydein plasma and tissues by High-Performance Liquid Chromatography and Thiobarbituric acid assay. Fırat Tıp Dergisi. 11(2): 88-92
[14] Gajski, G. and V. Garaj-Vrhovac, 2009. Radioprotective effects of honeybee venom (Apis mellifera) against 915-MHz microwave radiation-induced DNA damage in Wistar rat lymphocytes: in vitro study. Int.J Toxicol., 28(2): 88-98.
[15] Suh, S.J., K.S. Kim, M.J. Kim, Y.C. Cang, S.D. Lee, M.S. Kim, D.Y. Kwon and C.H. Kim, 2006. Effects of bee venom on protease activities and free radical damages in synovial fluid from type П collagen-induced rheumatoid arthritis rats. Toxicol In Vitro., 20(8): 1465-1471
[16] Park, H.J., D.J. Son, C.W. Lee, M.S. Choi, U.S. Lee, H.S. Song, J.M. Lee and J.T. Hong, 2007. Melittin inhibits inflammatory target gene expression and mediator generation via interaction with Ikappa B kinase. Biochem. Pharmacol., 73: 237-247.

Thank you for copying data from http://www.arastirmax.com