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Decolourisation of Reactive Dyes Using Bacterial Isolates Recovered from Textile Effluent in Lagos State

Journal Name:

  • International Journal of Science and Engineering Investigations

Publication Year:

  • 2017

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Author Name
Abstract (2. Language): 
A serious problem have been posed to the ecosystem due to untreated or partially treated waste water and industrial effluent discharges into our natural ecosystem and the life forms. Effluent samples were collected from three industrial textile industries in Lagos State, Nigeria. The total heterotrophic count of bacteria was determined using pour plate method on Plate Count Agar (PCA). Pure isolates were subjected to the solid phase screening medium. Pure isolates from the solid phase were subjected to liquid screening phase and potential isolates from the liquid screening phase were used for the decolourisation experiments. For the decolourisation experiments, potential isolates were inoculated into Mineral Salt Medium (MSM) supplemented with 0.1mg/L of reactive black, reactive red and reactive yellow dye. Decolourisation activity was determined by spectrophotometer. The result of the Total Heterotrophic Bacterial Count (THBC) of textile effluents showed that NHT at Ikorodu had the highest bacterial count of 2.85× 108 CFU/mL while SWL at Ikeja had the lowest count of 1.13 × 108 CFU/mL. Fourteen isolates were able to grow on Luria- Bertani Agar medium (LB) supplemented with 0.1g/L in the solid phase method. Four isolates were chosen as potential isolates from the liquid phase and were used for decolourisation experiments. In the decolourisation experiments, klebsiella oxytoca after 5 days also had the highest decolourisation potential of 70% for reactive black while Bacillus firmus had the least decolourisation activity of 25%. Decolourisation of reactive red Her dye after 5days showed that Staphylococcus aureus had the highest decolourisation of 45% while Bacillus firmus had the lowest decolourisation potential of 14%. Bacillus macerans after 5 days showed the highest reactive yellow He4g dye decolourisation of 21% while Klebsiella oxytoca had the least decolourisation activity of 13%. The present showed the potential of different isolates to decolorize dye at different levels which would help in solving the problem of pollution
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REFERENCES

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K. oxytoca
B. firmus
S. aureus
B.macerans
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International Journal of Science and Engineering Investigations, Volume 6, Issue 61, February 2017 105
www.IJSEI.com Paper ID: 66117-13
ISSN: 2251-8843
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