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OKSİDATİF STRES GÖSTERGESİ OLARAK SERUMDA TOTAL SÜLFİDRİL DÜZEYLERİ VE GLUTATYON PEROKSİDAZ AKTİVİTESİ

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2000

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Abstract (2. Language): 
Total thiol levels and glutathione pcroxidase activity as parameters of axidative stress. Free radicals play an important role in the pathogenesis of severai diseases. We invcstigated prooxi-dant-antioxidant balance İn serum of oid men and of patients with coronary artery diseases, coloreetal and prostat cancer. For fhis reason, malondialdehyde (MDA), antioxidant activity (AOA), total thiol groups and glutathione peroxidasc (GSH-Px) activity were measurcd in serum. Although serum MDA levels and AOA did not change, total thiol groups and GSH-Px activity were found to decrease in ali patients. These results m ay indicate that the determinati-on of total thiol groups and GSH- Px activity may be used as markers to investigate prooxi-dant-antioxidant balance in serum.
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Abstract (Original Language): 
Serbest radikaller birçok hastalığın etyopatojenezinde etkin bir rol oynamaktadır. Çalışmamızda yaşlı kişilerde, koroner arter hastalarında, kolon ve prostat kanserli hastalarda prooksidan-antioksidan denge incelendi. Bu amaçla serumda malondİaldehit (MDA) düzeyleri, antioksi-dan aktivite (AOA), total sülfidrİI içeriği (t-SH) ve glutatyon peroksidaz aktivitesi (GSH-Px) ölçüldü. Serum MDA ve AOA incelediğimiz hasta gruplarında değinmemekle birlikte, tüm gruplarda serum GSH-Px aktivitesi ve t-SH içeriğinde anlamlı bir azalma saptandı. Sonuçlarımıza göre, serumda GSH-Px aktivitesi ve t-SH içeriğini belirlemenin prooksidan-antioksidan dengeyi incelemede yararlı bir gösterge olabileceği ileri sürülebilir
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  • 3
264-267
  • Turkish

REFERENCES

References: 

Bulur H, Özdcmirler G, Öz B, Toker G, Öztürk M, Uysal M: High cholesteroî diet supplenıented with sunflowcr sced oil but not olive oil stimulates lipid peroxidation in plasma, liver and aorta of rats. Nufr Biochem 6:547 (1995).
2. Doğru-Abbasoğlu S, Aykaç-Toker G, Koçak T, Ünlüer E, Uysal M: Anlioxidant enzyme activities with benign prostatİc hyperpüısia or prostatc cancer are not predicti-ve. J Cancer Res Clin Oncol 125:402 (1999).
3. Doğru-Abbasoğlu S, Kanbağlı Ö, Buîur H, Babalık E, Öztürk S, Aykaç-Toker G, Uysal M: Lipid peroxides and aııtioxidant status in serum of patients with angioraphi-cally defined coronary atherosclerosis. Clin Biochem 32:ö7İ (1999).
4. Doğru-Abbasoğlu S, Tamer-Toplani S, Uğurnal B, Ko-çak-Tokcr N, Aykaç-Toker G, Uysal M: Lipid peroxida-lion and an£ioxidant enzymes in livers and brains of aged rats. Mcch Age Develop 98:177 (1997).
5. Gutteridge JMC: Lipid peroxidation and antioxidants as biomarkers of tissue damagc. Clin Chem 41:1819 (1995).
6. tlailiwell B. Gutteridge JMC: Free Radicals in Bİulogy and Medicine, Oxford Science Publications,Oxford, 3. Baskı (1999).
Kanbağlı Ö., Doğru-Abbasoğlu S., Özdemirler G., Aykaç-Toker G., Uysal M.
7. Hu ML: Measurement of protein thiol groups and glutathione İn plasma. Meîhod Enzymol 233:380 (S994).
8. Kanbağlı Ö, Özdemirler G, Bulut T, Aykaç-Toker G, Uysal M: Mitocondrial lipid peroxedes and antioxidant enzymes in colorectal adenocarcinoma tissues. Japan J Cancer Res (Baskıda).
9. Knighl JA Diseases related to oxygen-derived free radicals. Ann Clin. Lab Sci 25:11 (1995).
10. Mehmetçik G, Özdemirler G, Kanbağlı Ö, Toker G, Uysal M: Age-related changes in plasma lipid peroxidation and anlicoridant syslem in humans and rats. Arch Geron-lol Gerialr 25:305 (1997).
11. Miller NJ, Rice-Evans Ca: Spectrophotometric determi-nation of antioxidant activity, Redox Report 2:161 (1996).
[2. Moorc JA, Noİva R, Wells İC: Selenium concentratİons in plasma of patients with arteriographically defined co-ronary atheroselerosis. Clin Chem 30:1171 (1984).
13. Özdemirler G, Pabııççuoğlu H, Bulut T, Buğra D, Uysal M, Tokcr G: Incıcased lipid peroxide ievels and anlioxi-dant system in coloreeta! cancer. J Cancer Clin Oncol 124:555 (1998).
14. Paglia DE, Valentine WN: Studies on quantitive and qu-alitive characterization of erytrhocyte glutathione peroxi-dase. J Lab Clin Med 70:158 (1967).
15. Porter M, Pearson DJ, Suarez-Mendez VJ Blann D: Plasma platelet and erytrhocyte glutathione peroxidases as risk factors in isehemie heart disease in man. Clin Sci 83:343(1992).
16. Psathakis D, Wedemeyer N, Oevermann E, Krug F, Sie-gers CP, Bruch HP: Blood selenium and glutathione pe-roxidase status in patients with colorectal cancer. Dis Co-lon Rectum 41:328 (1998).
17. Rzymowska J: Activtties of enzyme transducing ext-racellular signals-gamma glutamyltransferase and enzymes metabolizind glutathione in acute lymphoblastic and myeloid human leukemias. Neoplasma 42:53 (1995).
18. Sattar N, Scott HR, McMillan DC, Talwar D, O'Reilly DS, Fell GS: Acute-phase reactants and plasma trace element concentratİons in non-small celi hıng cancer patients and controls. Nutr Cancer 28:308 (1997).
19. Stockcr R, Frei B: Endogcnous antioxidarıt defences İn human blood plasma,"Oxidative Stı*ess: Oxidants and Antioxidants, editör: Sies H, Acedemic Press, London (1991)", sayfa: 213.
20. Stocks J, Gutteridge JMC, Sharo KJ, Domıandy JL: As-say using brain homogenate for measuring the antioxi-dant activity of biologicai fluids. Ciin Sci Mol Med 47:215(1974).
21. Wasowicz W, Ne've J, Peretz A: Optimized steps in fluo-rometric determinalion of thiobarbituric acid-reactive substances in serum: impoıtancc of extraction pH and influence of sarnple preservation and storage. Ciin Chem 39:2522 (1993).
22. Yagi K, Nishigaki, Ohama H: Measurement of serum TBA value. Vitamins (Japan) 37:105 (1968).
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