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DİFFÜZ ASTROSİTOMA OLGULARINDA DNA İÇERİK TAYİNİ

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Determinaficm of DNA contentwithin astroeytoma cases. The aİm of this study was to evalua-te whcther the DNA index and/or proliferative indcx ıneasured by the flow cytoınetry (FCM) and image analysis (İA) is proportionai to histopathological grading of diffuse astroeytomas. Intracerebral diffuse astroeytomas (n=29) werc graded according to Daumos-Duport criteria, six of which were Grade 2, fhree were Grade 3 and twcnty were Grade 4. DNA index and proliferative index were calculated by using Coulter M plus program for FCM and Samba-Ploİdy 4000 program for İA. Twentythrce out of 29 cases were found to have the same ploidy by both of the techniques. The percentage of the proliferating cells were found to be 11.0+6.0 in Grade 2, 11.4+5.4 in Grade 3 and 17.0+17.2 in Grade 4 with FCM. The proliferative index were found to be 34.6+19.9 in Grade 2, 26.6+16.0 in Grade 3 and 29.3+18.5 in Grade 4 with IA. The DNA indices were found to be 1.31 ±0.49 in Grade 2, 1.10+0.40 in Grade 3 and 1.57+0.66 in Grade 4 with FCM. The DNA indices were found to be 1.35±0.54 in Grade 2, 2.40+0.86 in Grade 3 and 1.37±0.60 in Grade 4 with IA. When these two tcchniques werc compared, any statisticaily significant difference could not be determined in terms of distinguishing the histopathological groups of different grades from each other. According to the results of this study; the FCM and IA tecbniques are not proportionai to the quantitative histopathology and cannot be used for grading of diffuse astroeytomas.
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Abstract (Original Language): 
Bu çalışmanın amacı diffüz astrositoma oigularmda flow sitometre (FCM) ve görüntü analizi sistemi (GAS) yöntemi ile ölçülen DNA ploidi indeksi ve/veya proliferatİf indeksin bistopato lojik gradelemc ile uyumlu olup olmadığını incelemektir. Toplam 29 adet intrascrcbral diffuz astrositoma olgusu Daumas-Duport sınıflamasına göre gradelcndirikli. Altı vaka Grade 2, üç vaka Grade 3 ve yirmi vaka Grade 4 olarak bulundu. Çalışmada flow sİtometrede (FCM) Co-ulter M Plus programı kullanılarak, GAS ise Samba-Ploidy 4000 yazılım programı kullanılarak DNA indeksi (Dİ) ve proliferatİf indeks hesaplandı. Çalışılan 29 vakanın 23 tanesinde FCM vc GAS yöntemleri ile DNA ploidileri aynı bulundu. FCM'de; proliferatİf indeks açısından incelendiğinde ortalama vc standart sapmalar; Grade 2 için 11.0+6.0, Grade 3 için 1 î .4±5.4, Grade 4 için 17.0±17.2 olarak ölçüldü. GAS'dc bu değerler; Grade 2 için 34.6Ü9.9, Grade 3 için 26.6Ü6.0, Grade 4 için 29.3+18.5 olarak ölçüldü. FCM'de; DNA indeksi açısından incelendiğinde ortalama ve Standard sapmalar Grade 2 için 1.31+0.49, Grade 3 için 1.10+0.40, Grade 4 için ise 1.57+0.66 olarak saptandı. GAS yönteminde bu değerler, Grade 2 için 1.35+0.54; Grade 3 için 2.4+0.86; Grade 4 için ise 1.37+0.60 olarak bulundu. DNA indeksi ve proliferatİf İndeks FCM yöntemiyle ve GAS yöntemiyle Ölçüldüğü zaman gruplar arasında istatistiksel olarak anlamlı bir fark bulunmadı. Sonuç olarak her iki yöntemin de tek başına veya birlikte kullanıldıkları zaman diffüz as tros i tornaların histopatolojik gradelemesi İle uyumlu olmadıkları tespit edildi ve gradelendİrmede kullan ilamayacaklan Öne sürüldü.
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  • 2
83-91
  • Turkish

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