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PREEKLAMPSİDE PLAZMA PRO-İNFLAMATUAR VE ANTİ-İNFLAMATUAR SİTOKİN DÜZEYLERİ

PLASMA PRO-INFLAMMATORYAND ANTI-INFLAMMATORY CYTOKINE LEVELS IN PREECLAMPSIA

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Cytokines appear to contribute to the maintenance of normal pregnancy and development of pathological conditions. The aim of this study was to evaluate plasma cytokine pattern in preeclampsia and whether there is any relationship with disease severity or not. Materials and methods: Plasma samples of 65 women (25 healthy nonpregnant (NP), 20 normotensive pregnant (NTP) and 20 preeclamptic pregnant (PEP) women) were investigated by means of TNFα, IL-1β, IL-12, IL-4 and IL-10 concentrations. Statistical significance was analysed by Student’s t-, Kruskal- Wallis, Mann-Whitney U- and Spearman correlation tests. Results: In PEP in comparison with NP women, together with elevation of pro-inflammatory TNFα, IL-1β and IL-12 (Th1 cytokines), there is an increase of anti-inflammatory IL-4 and IL-10 (Th2 cytokines). In addition, there is an increase of anti-inflammatory IL-4 and IL-10 levels in PEP in comparison with NTP. Among the preeclamptic group there is a significant positive correlation between IL-1β/IL-10 and TNFα/IL-12; also, a significant negative correlation between IL-4/systolic blood pressure, IL-4/LDH. Conclusions: The rise of anti-inflammatory cytokines probably is a compensatory mechanism by which IL-4 and IL-10 counteract to pro-inflammatory cytokines, and thus balance their endothelium destroying effects. The presence of negative correlation between anti-inflammatory cytokines and blood pressure or some liver enzymes suggests that there is probably a relationship between plasma cytokines and disease severity. On the other hand, elevated maternal plasma IL-4 and IL-10 concentrations in preeclampsia may be a response to improve altered Th1/Th2 balance and to protect from maternal immunorejection
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Abstract (Original Language): 
Amaç: Sitokinler normal gebeli¤in sürdürülmesi ve patolojik durumlar›n ortaya ç›kmas›nda önemli rol oynar. Bu çal›flmada preeklampside plazma sitokin paternini incelemek ve hastal›¤›n seyri ile bir iliflkisi olup olmad›¤›n› de- ¤erlendirmek hedeflenmifltir. Gereç ve yöntem: 25’i sa¤l›kl› ve gebe olmayan (NP), 20’si normotensif gebe (NTP), ve 20’si preeklamptik gebe (PEP) olmak üzere toplam 65 kad›n›n plazma örneklerinde TNFα, IL-1β, IL-12, IL-4 ve IL-10 konsantrasyonlar› tayin edildi. ‹statistiksel de¤erlendirme Student’s t-, Kruskal-Wallis, Mann-Whitney U- ve Spearman korelasyon testleri ile gerçeklefltirildi. Bulgular: NP kad›nlara k›yasla, PEP’de pro-inflamatuar ve Th1 sitokinleri olan TNFα, IL-1β, IL-12 art›fl› ile birlikte anti-inflamatuar ve Th2 sitokinleri olan IL-4 ve IL-10 de artmaktad›r. Bununla birlikte, NTP’ye k›yasla PEP’de anti-inflamatuar IL-4 ve IL-10 düzeyleri artmaktad›r. Preeklampsi grubunda IL-1β/ IL-10; TNFα/IL-12 aras›nda pozitif; IL-4/sistolik kan bas›nc› ve IL-4/LDH aras›nda ise negatif korelasyon bulundu. Sonuçlar: Anti-inflamatuar sitokin art›fl› pro-inflamatuar sitokinlerin neden oldu¤u endotel disfonksiyonuna karfl› koyan bir kompensatuar mekanizma olabilir. Anti-inflamatuar sitokinler ile kan bas›nc› veya baz› karaci¤er enzimleri aras›nda bulunan negatif korelasyon, plazma sitokin düzeyleri ve hastal›¤›n seyri aras›nda bir iliflki olabilece¤ini ortaya koymaktad›r. Bununla birlikte, preeklampside anne plazmas›nda IL-4 ve IL-10 konsantrasyonlar›n›n art›- fl›n›n, Th1/Th2 sitokin dengesini etkileyerek, maternal imünorejeksiyondan korumaya çal›flan bir cevap oldu¤unu söyleyebiliriz.
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