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ENDOTOKSİN/D-GALAKTOZAMİN (LPS/GaIN) VERİLEN SIÇANLARIN ERİTROSİT ZARLARINDA LİPİT VE PROTEİN DEĞİŞİKLİKLERİ

THE ALTERATIONS IN LIPID AND PROTEINS IN ERYTHROCYTE MEMBRANES OF ENDOTOXIN/D-GALACTOSAMINE (LPS/GaIN)-ADMINISTERED RATS

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Amaç: Endotoksin/D-galaktozamin (LPS/GaIN)’in sıçan eritrosit zarı üzerine etkilerini aydınlatmak. Gereç ve yöntem: Erkek Wistar sıçanlar, intraperitoneal 200 mg/kg D-galaktozamin (GaIN) ve 200 μg/ kg lipopolisakkarit (LPS) uygulamasından 18 saat sonra kesilerek ultrasantrifüj yöntemi ile eritrosit zarı (ghost) elde edildi. Sıçanların eritrosit zarlarında, malondialdehit (MDA), sülfidril (-SH) ve protein karbonil (PC) içeriği ve Na+,K+-ATPaz aktivitesi spektrofotometrik olarak ölçüldü. MDA ve PC sıçanların plazmalarında da tayin edildi. Bunun yanısıra, eritrosit zar iskeletinin proteinlerindeki değiĢiklikler sodyum dodesil sülfat gel elektroforezi (SDS-PAGE) tekniği ile saptandı. Bulgular: LPS/GaIN uygulamasının, erkek Wistar sıçanlarda, serum alanin transaminaz (ALT) ve aspartat transaminaz (AST) ölçümleri sonucu, hepatit oluĢturduğu belirlendi. Sıçanların plazma MDA düzeylerinde artıĢ bulunurken, eritrosit zarlarında MDA ve PC düzeylerinin değiĢmediği gözlendi. Bununla birlikte sıçan eritrosit zarlarının –SH içeriği ve Na+,K+-ATPaz aktivitesinde azalma olduğu saptandı. Eritrosit zar iskeleti proteinlerinin SDS-PAGE örneklerinde ise, proteinlerin bir kısmında, özellikle de, α-spektrin ve β-spektrin band yoğunluklarında azalmalar olduğu gözlendi. Sonuç: Bulgularımız, Na+,K+-ATPaz aktivitesindeki azalmadan zar –SH içeriğindeki azalmanın sorumlu olabileceğini düĢündürmektedir. Bunun yanısıra, eritrosit zar iskeletinin proteinlerinde görülen değiĢiklikler, hücre zar yapısında ve dolayısıyla fonksiyonlarında anormalliklere neden olarak LPS/GaIN hepatotoksisitesine katkıda bulunabilir.
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Abstract (Original Language): 
Objective: To clarify the effects of endotoxin/D-galactosamine (LPS/GaIN) on erythrocyte membranes (ghost) of rats. Materials and methods: After 18 hours of intraperitoneal coadministration of 200 mg/kg D-galactosamine (GaIN) and 200 μg/kg lipopolysaccharide (LPS), male Wistar rats were decapitated and ghost was prepared by ultracentrifugation. Malondialdehyde (MDA), sulfhydryl (-SH) and protein carbonyl (PC) contents and Na+,K+-ATPase activity were measured in ghost, spectrophotometrically. MDA and PC were measured in also plasma of rats. Furthermore, sodium dodesyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) technique was used for the determination of the modifications in cytoskeletal ghost proteins . Results: LPS/ GaIN administration resulted in hepatitis in male Wistar rats as evaluated by assessments of serum alanine transaminase (ALT) and aspartate transaminase (AST) activities. While plasma MDA levels were determined to increase, ghost MDA and PC contents were observed unchanged. However, -SH content as well as Na+,K+-ATPase activity of ghost were found decreased. As shown by the electrophoretic gel patterns, significant reduction in the intensities of some cytoskelatal membrane proteins, especially in α–spectrin and β–spectrin, was observed in erythroctes of LPS/ GaIN-administered rats. Conclusion: From these findings, we concluded that reduced Na+,K+-ATPase activity may be associated with the diminished –SH content in ghost of LPS/GaIN-administered rats. Furthermore, alterations seen in cytoskeletal proteins of ghost may participate to LPS/GaIN-induced hepatotoxicity by causing the deformation of cell membrane structure and abnormality of membrane functions, ultimately.
FULL TEXT (PDF): 
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  • 4
121-124
  • English

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