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Türkiye'nin Batısındaki Gökkuşağı Alabalık Yetiştirme Çiftliklerinde Yersinia Ruckeri'nin PZR Kullanılarak Tanımlanması

DETECTION OF YERSINIA RUCKERIBY PCR IN RAINBOW TROUT (ONCORHYNCHUS MYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY

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Abstract (2. Language): 
The presence of Yersinia ruckeriin rainbow trout hatchery farms was investigated by PCR Assay. Twenty-five rainbow trout, suspected of Enteric Redmouth Disease, were examined in this study. Yersinia ruckeristrains were identified from 8 out of 25 samples (32%). Ten of 25 (40%) rainbow trout samples showed positive reactions for Yersinia ruckeriin the PCR. All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri
Abstract (Original Language): 
Bu araştırmada, GökkuşağıAlabalığıçiftliklerindeki Yersinia ruckerivarlığıPolimeraz Zincir Reaksiyonu (PZR) metodu ile incelendi. Bu çalışmada Kızıl Ağız Hastalığı şüpheli 25 adet GökkuşağıAlabalığı materyal olarak kullanıldı. 25 örneğin 8 (% 32)’inden Yersinia ruckeriidentifiye edildi. 25 örneğin 10’unda PZR’da Yersinia ruckeriyönünden pozitiflik saptandı. PZR pozitif ürünlerin hepsinde, Yersinia ruckeriiçin spesifik 575 bp uzunluğunda bantlar elde edildi
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