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Koyun Oositlerinin Partenogenetik Aktivasyonu

PARTHENOGENETİC ACTIVATION OFSHEEP OOCYTES

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
: In order to establish somatic- and embryonic-cell nuclear transfer procedures in sheep in our laboratory, two activation protocols for parthenogenetic activation of sheep oocytes were tested. Oocytes obtained from ovaries of slaughteredewes were matured for 24 h in medium 199 supplemented with pyruvate, hormones (FSH and LH) and 10% FCS. After maturation, all oocytes were exposed to single electrical stimulation of 1.25 kV/cm for 80 µsec, then placed into ionomycine and cytochalasin B for 5 min and 30 min respectively, and then divided into 2 groups randomly. While oocytes in Group I were incubated in 6-dimethylaminopurine (6-DMAP) for 2 h, oocytes in Group II were kept in cycloheximide (CHX) for 3 h before transferring into culture medium. Cleavage and blastocyst rates in groups I and II were 82.7% and 81.3%, and 2.3% and 0% respectively (P>0.05). These are our preliminary results for the parthenogenetic development of sheep oocytes in Turkey and represent promising results in the direction to achieve sheep cloning.
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Abstract (Original Language): 
Koyunlarda somatik ve embriyonik hücre nüklear transfer yöntemlerini laboratuvarımızda başlatabilmek için, iki farklıpartenogenetik aktivasyon protokolü denendi. Oositler mezbahada kesilen koyunların ovaryumlarından elde edildi ve piruvat, hormonlar (FSH ve LH) ve %10 FCS katkılımedyum 199 içerisinde 24 saat olgunlaştırıldı. Olgunlaştırılan oositlere 80 µsec süreyle 1.25 kV/cm elektrik akımıverildi ve oositlerin tamamıönce 5 dk ionomisin içerisinde, daha sonra ise 30 dk sitokalasin B içerisinde bırakıldı. İki gruba rastgele ayrılan oositler, 1. grupta 6-DMAP’lımedyumda 2 saat, 2. grupta ise sikloheksimid’li medyumda 3 saat inkübe edildikten sonra kültür medyumuna aktarıldı. Grup I ve II’de elde edilen embriyolarda yarıklanma oranlarısırasıyla %82.7 ve %81.3 olurken blastosist oranları%2.3 ve %0 olarak bulundu (P>0.05). Sunulan çalışma koyunların klonlanmasıkonusunda önemli aşamalardan birini oluşturan partenogenetik aktivasyon konusunda ülkemizde gerçekleştirilmişilk çalışmadır.
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  • Turkish

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