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Domuzlarda Actinobacillus pleuropneumoniae Varlığının PCR ile Araştırılması

INVESTIGATION OF THE PRESENCE OF ACTINOBACILLUS PLEUROPNEUMONIAE BY PCR IN PIGS

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Abstract (2. Language): 
A. pleuropneumoniae specific DNA in tonsil samples of 1 month to 3 years old pigs raised in different regions and send to slaughterhouses was investigated by PCR using primers (5 -’GATAAACCTTTTCCGGAATT-3’ and 5’-TACCACACCGTGTTTATCAA-3’) derived from dsbE like gen. In neither tonsil sample, 342 bp bands were observed by PCR. Determining the samples as PCR negative is promising that the infection is not present in the investigated farms and further studies should be performed for this and other possible infectious diseases of pigs.
Abstract (Original Language): 
Türkiye’nin farklı bölgelerinde yetiştirilerek kesime gönderilen 1 ay - 3 yaş arasındaki 312 domuz’a ait tonsil örneğinde Actinobacillus pleuropneumoniae spesifik DNA’sı, dsbE like geninden derive primerler (5’GATAAACCTTTTCCGGAATT-3’ ve 5’-TACCACACCGTGTTTATCAA-3’) kullanılarak PCR ile araştırıldı. PCR sonucunda tonsil örneklerinin hiç birinde 342 bp’lik bantlar görülmedi. Örneklerin PCR negatif olarak saptanması, domuz pleuropnömonisinin incelenen işletmelerde bulunmadığını ortaya koyması yönünden sevindiricidir. Bu ve diğer infeksiyöz hastalıklar açısından yeni çalışmaların yapılması gerekmektedir.
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