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AŞILANMIŞ SAHİPLİ VE SAHİPSİZ KÖPEKLERDE KUDUZ VİRUSUNA KARŞI OLUŞMUŞ NÖTRALİZAN ANTİKOR SEVİYELERİNİN SAPTANMASI

Detection of Neutralising Antibody Titration in Vaccinated Owned and Stray Dogs against Rabies Virus

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Abstract (2. Language): 
The aim of this study was to determine the titers of neutralising antibodies against rabies virus in five hundred vaccinated owned and stray dogs within the Istanbul province. The levels of the neutralising antibody titration in the blood serum were evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) method. Sixty five (13%) of the dogs examined had adequate level (0.5 IU/ml and over) of antibody titration. Also, the effects on the formation of the neutralising antibody levels following the vaccination of some factors, such as being owned or stray, the number of repetition of vaccination, the age, living area, sex, interval between the last vaccination and sampling, have been evaluated statistically. As a result of the statistical evaluations, it has been determined that interval between the last vaccination and sampling had an important effect on formation of the neutralising antibody titration ( P<0.001). Also, it was determined that the effect of the age of the dog was also significant for the neutralising antibody levels (P<0.05). On contrary, being owned or stray, sex, living area and the number of repetition of the vaccination had no effects on the neutralising antibody levels.
Abstract (Original Language): 
Bu çalışmada, İstanbul İli sınırları içerisinde yaşayan, aşılanmış sahipli ve sahipsiz beşyüz köpekte kuduz virusuna karşı oluşmuş nötralizan antikor seviyelerinin saptanması amaçlandı. Kan serumlarında oluşmuş nötralizan antikor titreleri ELISA ile araştırıldı. İncelenen köpeklerin 65 tanesi (%13) yeterli düzeyde nötralizan antikor (0 ,5 IU/ml ve üzeri) titresine sahipti. Ayrıca aşılamayı takiben oluşmuş nötralizan antikor seviyelerine sahip lilik durumu, aşı tekrar sayısı, yaş, yaşam alanı, cinsiyet, son aşı ile örnekleme arası geçen süre gibi faktörlerin etkileri istatistiksel değerlendirme ile incelendi. İstatistiksel değerlendirmeler sonucunda, son aşı ile örnekleme arası geçen sürenin şekillenmiş nötralizan antikor titrelerine etkisinin önemli olduğu (P<0,001) belirlendi. Ayrıca köpek yaşlarının da nötralizan antikor seviyeleri için önemli olduğu saptandı (P<0,05). Buna karşın, sahiplilik durumu, cinsiyet, yaşam alanları ve aşı tekrar sayısının oluşmuş nötralizan antikor seviyeleri üzerine etkili olmadığı belirlendi.
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REFERENCES

References: 

ATAG, 2003. Australian Technical Advisory Group on
Immunisation of the Australian Government
Department of Health and Ageing. The
Australian Immunisation Handbook. 8
th
edition.
Commonwealth of Australia Department of
Health and Ageing, Australia, pp. 1-10
Bozkaya, Ç., 2000. Ankara’da soğuk zincir
donanımında sahip ecza depolarında aşı ve
diğer biyolojik maddelerin soğuk zincir
koşullarına uygun olarak saklama durumlarının
değerlendirilmesi. Master of Science Thesis,
University of Hacettepe. Insti tute of Medical
Science, Ankara.
Cliquet, F., Verdier, Y., Sagne, L., Aubert, M.,
Schereffer, J.L., Selve, M., Wasniewski, M.,
Servat, A., 2003. Neutralising antibody titration
in 25,000 sera of dogs and cats vaccinated
against rabies in France, in the framework of
the new regulations that offer an alternative to
quarantine. Revue Scientifique et Technique-Office International des Epizooties 22, 857- 866.
Delgado, S., Carmenes, P., 1997. Immune response
fo llowing a vaccination campaign against rabies
in dogs from northwestern Spa in. Preventive
Veterinary Medicine 31, 257-261.
Doymaz, M.Z.,2000. Medikal Viroloji. Nobel Tıp
Press, İstanbul.
EC, 2003. Regulation of the European Parliament and
of the Council on the animal health
requirements applicable to the non -commercial
movement of pet animals and amending
Council Directive 92/65/EEC (EC) No
998/2003. (Acce ssed 10/12/2010).
http://ec.europa.eu/food/animal/liveanimals/pets
/annex2c_reg998_03_en.pdf
Fooks, A.R., Mcelhinney, L.M., Brookes, S.M.,
Johnson, N., Keene, V., Parsons, G., Soldan,
A., 2002. Rabies antibody testing and the UK
Pet Travel Scheme. Veterinary Record 150,
428- 430.
Hogenesch, H., Thompson, S., Dunham, A., Ceddia,
M., Hayek, M., 2003. Effect of age on immune
parameters and the immune response of dogs to
vaccines: a cross - sectional study. Veterinary
Immunology and Immunopathology 94, 113-121.
Johnson, H.N., 1965. Rabies Virus. In Horsfall FL and
Tamm I. Viral and Rickettsial Infections of
Man. 4
th
edition. J. B. Lippincott Company,
Philadelphia, pp.814-835.
Kasempimolporn, S., Sichanasai, B., Saengseesom,
W., Puempumpanich, S., Chartraporn, S.,
Sitprija, V., 2007. Prevalance of rabies virus
infection and rabies antibody in stray dogs: A
survey in Bangkok, Thailand. Preventive
Veterinary Medicine 78, 325-332.
Kıyan, A., 2001. İstanbul İli Küçükçekmece İlçesi
Sağlık Grup Başkanlığ ı Bölgesinde Soğuk
Zincir. Thesis for Expertise, Istanbul University
Faculty of Medicine İstanbul.
Lala, M.K., Lala, K.R., 2003. Thermostability of
vacinnes. Indian Pediatrics 40, 311-319.
Lloyd, J., 2000. Technologies for vaccine delivery in
the 21
st
century. Switzerland Technologies
Team of the Department of Vaccines and
Biologicals, Geneva.
Mansfield, K.L., Burr, P.D., Snodgrass, D.R.,
Sayers, R., Fooks, A.R., 2004. Factors
affecting the serological response o f dogs and
cats to rabies vaccination. Veterinary Record
154, 423-426.
MARA, 2010. Ministry of Agriculture and Rural
Affairs. Contestation Program with Animal
Diseases and Injurious 2010/02. Ministry of
Agriculture and Rural Affairs General
Directorate of Protection a nd Control,
http://www.kkgm.gov.tr/mev/genelge.html
(Erişim 10/12/2010)
Özdamar, K., 1999. Statisitcs Data Analysis with
Package Program SPSS, I. Kaan Press,
Eskişehir, pp.62.
Rupprecht, C.E.; Hanlon, C.A., He machudha, T.,
2002. Rabies re-examined. The Lancet Infection
Disease 2, 327- 343.
Sage, G , Khawplod, P., Wilde, H., Lobaugh, C.,
Hemachudha, T., Te psumethanon, W.,
Lumlertdaecha B., 1993. Immune response to
rabies vaccine in Alaskan dogs: failure to
achieve a consistently protective antibody
response. Transactions of the Royal Socıety of
Tropical Medicine And Hygiene 87(5), 593- 5.
Shimazaki, Y., Inoue, S., Takahashi, C., Gamoh, K.,
Etol, M., Kamiyama, T., Makie, H., 2003.
Immune response to Japanese Rabies Vaccine
in Domestic Dogs. Journal of Veterinary
Medicine B 50, 95-98.
Sugiyama, M., Yoshiki, R., Tatsuno, Y., Hiraga, S.,
Itoh, O., Gamoh, K., Minamoto, N., 1997. A
new Competitive Enzyme-Linked
Immunosorbent Assay demonstrates adequate
immune levels to rabies virus in compulsorily
vaccinated Japanese domestic dogs. Clinical
and Diagnostic Laboratory Immunology 4, 727-730.
WHO, 1992. Expert Committee on Rabies 8
th
Report.
WHO Technical Report Series No:824.
http://www.who.int/rabies/en/WHO_Expert_Co
mmittee_8th_report.pdf (Accessed
10/12/2010).
WHO, 1998. Oral Immunization of dogs against
rabies. Report of the sixth WHO constultation.
Zoonotic Diseases Division of Emerging and
other Communicable Disease Surveillance and
Control (EMC), G eneva, Switzerland.
WHO, 2004. Expert Consultation on Rabies First
Report. WHO Technical Report Series No:931.
http://whqlibdoc.w ho.int/trs/WHO_TRS_931_e
ng.pdf (Accessed 10/12/2010).
WHO, 2006. Temperature sensivity of vaccines.
Quality, Safety and Standarts (QSS) team of
the Department of Immunization, Vaccines and
Biologicals, Geneva, Switzerland.
WHO, 2008. Rabies Bulletin Europe. Volume 32.
Distribution of Rabies in Europe. Summary of
rabie s cases in Europe 3rd quarter.
http://www.who-rabies -bulletin.org
/Journal/Archive/Bulletin_2008_4.pdf
(Accessed 10/12/2010).
WHO, 2009. Rabies Bulletin Europe. Volume 33.
Comparison of the reporting quarter (I/2009)
with the same quarter of the previous year
(I/2008). http://www.who-rabies
bulletin.org/Journal/Archive/Bulletin_2009_1
pdf (Access ed 10/12/2010).
WHO, 2010. Rabies.
http://www.who.int/mediacentre/factsheets/fs 99/en/
(Accessed 10/12/2010).
Verthelyi, D., Klinman, D.M., 2000. Sex hormones
correlate with the activity of cytokine- secreting
cells in vitro. Immunology 100, 384-390.

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